scholarly journals RATIOMETRIC NEAR-INFRARED FLUORESCENT PROBES FOR THE SENSITIVE DETECTION OF INTRACELLULAR pH AND BIO-THIOLS IN LIVE CELLS

2020 ◽  
Author(s):  
Shuai Xia
Keyword(s):  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Sensitive Detection ◽  
Live Cells

scholarly journals A Near-Infrared Fluorescent Probe Based on a FRET Rhodamine Donor Linked to a Cyanine Acceptor for Sensitive Detection of Intracellular pH Alternations

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2679 ◽  
Author(s):  
Yibin Zhang ◽  
Jianheng Bi ◽  
Shuai Xia ◽  
Wafa Mazi ◽  
Shulin Wan ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Solution Ph ◽  
Acidic Conditions ◽  
Ph Level ◽  
Rhodamine Dye

A fluorescence resonance energy transfer (FRET)-based near-infrared fluorescent probe (B+) for double-checked sensitive detection of intracellular pH changes has been synthesized by binding a near-infrared rhodamine donor to a near-infrared cyanine acceptor through robust C-N bonds via a nucleophilic substitution reaction. To demonstrate the double-checked advantages of probe B+, a near-infrared probe (A) was also prepared by modification of a near-infrared rhodamine dye with ethylenediamine to produce a closed spirolactam residue. Under basic conditions, probe B+ shows only weak fluorescence from the cyanine acceptor while probe A displays nonfluorescence due to retention of the closed spirolactam form of the rhodamine moiety. Upon decrease in solution pH level, probe B+ exhibits a gradual fluorescence increase from rhodamine and cyanine constituents at 623 nm and 743 nm respectively, whereas probe A displays fluorescence increase at 623 nm on the rhodamine moiety as acidic conditions leads to the rupture of the probe spirolactam rings. Probes A and B+ have successfully been used to monitor intracellular pH alternations and possess pKa values of 5.15 and 7.80, respectively.

scholarly journals New near-infrared rhodamine dyes with large Stokes shifts for sensitive sensing of intracellular pH changes and fluctuations

2018 ◽  
Vol 54 (55) ◽  
pp. 7625-7628 ◽  
Author(s):  
Yibin Zhang ◽  
Shuai Xia ◽  
Mingxi Fang ◽  
Wafa Mazi ◽  
Yanbo Zeng ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Near Infrared ◽  
Sensitive Detection ◽  
Amine Group ◽  
Ph Changes ◽  
Stokes Shifts ◽  
Rhodamine Dyes

New near-infrared rhodamine dyes with large Stokes shifts were developed and applied for sensitive detection of cellular pH changes and fluctuations by incorporating an additional amine group with fused rings.

Turn-on visible and ratiometric near-infrared fluorescent probes for distinction endogenous esterases and chymotrypsins in live cells

2020 ◽  
Vol 306 ◽  
pp. 127567 ◽  
Author(s):  
Jiemin Wang ◽  
Zhidong Teng ◽  
Ting Cao ◽  
Jing Qian ◽  
Lei Zheng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Turn On

Novel near-infrared pH-sensitive cyanine-based fluorescent probes for intracellular pH monitoring

2019 ◽  
Vol 170 ◽  
pp. 107612 ◽  
Author(s):  
Di Jin ◽  
Bowei Wang ◽  
Yuqing Hou ◽  
Yuchao Du ◽  
Xue Li ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Ph Monitoring ◽  
Ph Sensitive

scholarly journals Detecting Zn(II) Ions in Live Cells with Near-Infrared Fluorescent Probes

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1592 ◽  
Author(s):  
Fang ◽  
Xia ◽  
Bi ◽  
Wigstrom ◽  
Valenzano ◽  
...  
Keyword(s):  
Detection Limit ◽  
Oxygen Atom ◽  
Membrane Permeability ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Emission Spectra ◽  
Live Cells ◽  
Phenolic Oxygen ◽  
Acetyl Group ◽  
Low Cytotoxicity

Two near-infrared fluorescent probes (A and B) containing hemicyanine structures appended to dipicolylamine (DPA), and a dipicolylamine derivative where one pyridine was substituted with pyrazine, respectively, were synthesized and tested for the identification of Zn(II) ions in live cells. In both probes, an acetyl group is attached to the phenolic oxygen atom of the hemicyanine platform to decrease the probe fluorescence background. Probe A displays sensitive fluorescence responses and binds preferentially to Zn(II) ions over other metal ions such as Cd2+ ions with a low detection limit of 0.45 nM. In contrast, the emission spectra of probe B is not significantly affected if Zn(II) ions are added. Probe A possesses excellent membrane permeability and low cytotoxicity, allowing for sensitive imaging of both exogenously supplemented Zn(II) ions in live cells, and endogenously releases Zn(II) ions in cells after treatment of 2,2-dithiodipyridine.

Novel far-visible and near-infrared pH probes based on styrylcyanine for imaging intracellular pH in live cells

2012 ◽  
Vol 48 (91) ◽  
pp. 11202 ◽  
Author(s):  
Li Fan ◽  
Yue-Jun Fu ◽  
Qiao-Ling Liu ◽  
Dong-Tao Lu ◽  
Chuan Dong ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Near Infrared ◽  
Live Cells ◽  
Ph Probes

scholarly journals Luminescent Probes for Sensitive Detection of pH Changes in Live Cells through Two Near-Infrared Luminescence Channels

ACS Sensors ◽  
2017 ◽  
Vol 2 (7) ◽  
pp. 924-931 ◽  
Author(s):  
Shuwei Zhang ◽  
Tzu-Ho Chen ◽  
Hsien-Ming Lee ◽  
Jianheng Bi ◽  
Avik Ghosh ◽  
...  
Keyword(s):  
Near Infrared ◽  
Sensitive Detection ◽  
Live Cells ◽  
Luminescent Probes ◽  
Ph Changes ◽  
Infrared Luminescence

Near-infrared fluorescent probes based on piperazine-functionalized BODIPY dyes for sensitive detection of lysosomal pH

2015 ◽  
Vol 3 (10) ◽  
pp. 2173-2184 ◽  
Author(s):  
Jingtuo Zhang ◽  
Mu Yang ◽  
Cong Li ◽  
Nethaniah Dorh ◽  
Fei Xie ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Living Cells ◽  
Sensitive Detection ◽  
Lysosomal Ph

Three BODIPY-based near-infrared fluorescent probes have been synthesized, characterized, and evaluated for the sensing and imaging of lysosomal pH inside living cells.

scholarly journals Fluorescent probes for sensitive and selective detection of pH changes in live cells in visible and near-infrared channels

2017 ◽  
Vol 5 (48) ◽  
pp. 9579-9590 ◽  
Author(s):  
Mingxi Fang ◽  
Rashmi Adhikari ◽  
Jianheng Bi ◽  
Wafa Mazi ◽  
Nethaniah Dorh ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Live Cells ◽  
Selective Detection ◽  
Ph Changes ◽  
Ring Structures

Five fluorescent probes bearing coumarin moieties with spirolactam ring structures have been developed to detect pH changes in visible and near-infrared channels.

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