Use of CRISPR/Cas ribonucleoproteins for high throughput gene editing of induced pluripotent stem cells

Methods ◽  
2021 ◽  
Author(s):  
Qi Wang ◽  
Sueanne Chear ◽  
Kristof Wing ◽  
David Stellon ◽  
Minh Thuan Nguyen Tran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Gene Editing ◽  
Induced Pluripotent

Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells

2015 ◽  
Vol 12 (9) ◽  
pp. 885-892 ◽  
Author(s):  
Daniel Paull ◽  
Ana Sevilla ◽  
Hongyan Zhou ◽  
Aana Kim Hahn ◽  
Hesed Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells

2017 ◽  
Vol 89 (4) ◽  
pp. 2440-2448 ◽  
Author(s):  
Anna Baud ◽  
Frank Wessely ◽  
Francesca Mazzacuva ◽  
James McCormick ◽  
Stephane Camuzeaux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Gene editing and clonal isolation of human induced pluripotent stem cells using CRISPR/Cas9

Methods ◽  
2017 ◽  
Vol 121-122 ◽  
pp. 29-44 ◽  
Author(s):  
Saniye Yumlu ◽  
Jürgen Stumm ◽  
Sanum Bashir ◽  
Anne-Kathrin Dreyer ◽  
Pawel Lisowski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Gene Editing ◽  
Induced Pluripotent

scholarly journals Harnessing the Power of Induced Pluripotent Stem Cells and Gene Editing Technology: Therapeutic Implications in Hematological Malignancies

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2698
Author(s):  
Ishnoor Sidhu ◽  
Sonali P. Barwe ◽  
Raju K. Pillai ◽  
Anilkumar Gopalakrishnapillai
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Gene Editing ◽  
Molecular Mechanisms ◽  
Hematological Malignancies ◽  
Disease Modeling ◽  
Clonal Evolution ◽  
Induced Pluripotent ◽  
Ipsc Disease Modeling

In vitro modeling of hematological malignancies not only provides insights into the influence of genetic aberrations on cellular and molecular mechanisms involved in disease progression but also aids development and evaluation of therapeutic agents. Owing to their self-renewal and differentiation capacity, induced pluripotent stem cells (iPSCs) have emerged as a potential source of short in supply disease-specific human cells of the hematopoietic lineage. Patient-derived iPSCs can recapitulate the disease severity and spectrum of prognosis dictated by the genetic variation among patients and can be used for drug screening and studying clonal evolution. However, this approach lacks the ability to model the early phases of the disease leading to cancer. The advent of genetic editing technology has promoted the generation of precise isogenic iPSC disease models to address questions regarding the underlying genetic mechanism of disease initiation and progression. In this review, we discuss the use of iPSC disease modeling in hematological diseases, where there is lack of patient sample availability and/or difficulty of engraftment to generate animal models. Furthermore, we describe the power of combining iPSC and precise gene editing to elucidate the underlying mechanism of initiation and progression of various hematological malignancies. Finally, we discuss the power of iPSC disease modeling in developing and testing novel therapies in a high throughput setting.

scholarly journals High-throughput propagation of human prostate tissue from induced-pluripotent stem cells

2019 ◽  
Author(s):  
AC Hepburn ◽  
EL Curry ◽  
M Moad ◽  
RE Steele ◽  
OE Franco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Neuroendocrine Cells ◽  
Prostate Tissue ◽  
Urogenital Sinus ◽  
Human Prostate ◽  
Human Prostate Tissue ◽  
Induced Pluripotent

AbstractPrimary culture of human prostate organoids is slow, inefficient and laborious. To overcome this, we demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells, for the first time, enable generation of human prostate tissue in vivo and in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. This approach overcomes major limitations in primary cultures of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. These models provide new opportunities to study prostate development, homeostasis and disease.

scholarly journals Application of CRISPR/Cas9 to human-induced pluripotent stem cells: from gene editing to drug discovery

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Claudia De Masi ◽  
Paola Spitalieri ◽  
Michela Murdocca ◽  
Giuseppe Novelli ◽  
Federica Sangiuolo
Keyword(s):  
Stem Cells ◽  
Drug Discovery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Gene Editing ◽  
Induced Pluripotent
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