Phylogeny of Eutardigrada: New molecular data and their morphological support lead to the identification of new evolutionary lineages

2014 ◽  
Vol 76 ◽  
pp. 110-126 ◽  
Author(s):  
Roberto Bertolani ◽  
Roberto Guidetti ◽  
Trevor Marchioro ◽  
Tiziana Altiero ◽  
Lorena Rebecchi ◽  
...  
Keyword(s):  
Molecular Data ◽  
Evolutionary Lineages

scholarly journals Molecular and Chemical Markers to Illustrate the Complex Diversity of the Genus Lupinus (Fabaceae)

Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 263
Author(s):  
Kalina Bermúdez-Torres ◽  
Maxime Ferval ◽  
Arianna Michelle Hernández-Sánchez ◽  
Andreas Tei ◽  
Charles Gers ◽  
...  
Keyword(s):  
Geographic Origin ◽  
Molecular Data ◽  
Chemical Markers ◽  
Nuclear Marker ◽  
First Approximation ◽  
Combined Use ◽  
Evolutionary Radiation ◽  
Evolutionary Lineages ◽  
Generation Sequencing ◽  
Alkaloid Profiles

The potential of secondary metabolites as systematic markers to get new insights in an intricate phylogeny of a recent evolutionary radiation is explored. A chemosystematic study of the genus Lupinus (Fabaceae) was performed, using quinolizidine (QA) and piperidine alkaloids (ammodendrine) as diagnostic characters. Seven major QA and the piperidine alkaloid ammodendrine were found to be the most frequent compounds. Two groups were supported according to their geographic origin: an Old World/Atlantic American group and a West New World group and this pattern is concordant with molecular data (here, based on an original barcode approach using the nuclear marker ITS). However, QA profiles are less informative at the species level. Despite a lack of resolution within the two groups, the alkaloid profiles agree with well supported clades based on DNA molecular characters. The combined use of chemical and barcode genetic markers represents a viable alternative for separating recent evolutionary lineages to a first approximation without having to resort to an expensive and sophisticated molecular arsenal such as next generation sequencing.

A new species of Diporiphora from the Goneaway Tablelands of Western Queensland

Zootaxa ◽  
2012 ◽  
Vol 3556 (1) ◽  
pp. 39 ◽  
Author(s):  
PATRICK COUPER ◽  
JANE MELVILLE ◽  
ANGUS EMMOTT ◽  
STEPHANIE N. J. CHAPPLE
Keyword(s):  
New Species ◽  
Genetic Study ◽  
Morphological Study ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Protein Coding ◽  
Evolutionary Lineage ◽  
Body Patterns ◽  
A New Species ◽  
Evolutionary Lineages

Australia’s agamid genus Diporiphora is speciose and widespread, however, there remain significant taxonomicuncertainties within this group. Field collections across the range of Diporiphora continue to uncover undocumentedmorphological and ecological variation. A new morpho-type was collected from hard pebbly soils on Valetta Station,western Queensland, providing ample data for the description of a new species (Diporiphora ameliae sp. nov.). Weundertook a morphological study, integrated with a comprehensive genetic study to provide the phylogenetic placementand distinctiveness of the new species. Although superficially similar to Diporiphora winneckei, the new species ischaracterised by well developed ventral body patterns consisting of four longitudinal grey stripes on a cream backgroundand three distinctive dark V-shaped markings that converge anteriorly on the throat and gular area. Molecular data ispresented incorporating a ~1200 bp of the mtDNA protein-coding gene ND2 and five flanking tRNAs for 58 newsequences and 53 previously published sequences. Phylogenetic analyses of the molecular data strongly support the newspecies as an independent evolutionary lineage within Diporiphora. In addition, the molecular data also showed that thereis far greater diversity in Diporiphora winneckei sensu lato than was anticipated. Our results clearly indicate that there are at least three independent evolutionary lineages of D. winneckei-like dragons.

scholarly journals An integrated study of the biodiversity within the Pseudechiniscus suillus–facettalis group (Heterotardigrada: Echiniscidae)

2019 ◽  
Author(s):  
Michele Cesari ◽  
Martina Montanari ◽  
Reinhardt M Kristensen ◽  
Roberto Bertolani ◽  
Roberto Guidetti ◽  
...  
Keyword(s):  
Species Identification ◽  
Integrated Approach ◽  
Molecular Data ◽  
High Variability ◽  
Special Focus ◽  
The Family ◽  
Data Points ◽  
Integrated Study ◽  
Evolutionary Lineages ◽  
Very High

Abstract Pseudechiniscus is the second most species-rich genus in Heterotardigrada and in the family Echiniscidae. However, previous studies have pointed out polyphyly and heterogeneity in this taxon. The recent erection of the genus Acanthechiniscus was another step in making Pseudechiniscus monophyletic, but species identification is still problematic. The present investigation aims at clarifying biodiversity and taxonomy of Pseudechiniscus taxa, with a special focus on species pertaining to the so-called ‘suillus–facettalis group’, by using an integrated approach of morphological and molecular investigations. The analysis of sequences from specimens sampled in Europe and Asia confirms the monophyly of the genus Pseudechiniscus. Inside the genus, two main evolutionary lineages are recognizable: the P. novaezeelandiae lineage and the P. suillus–facettalis group lineage. Inside the P. suillus–facettalis group, COI molecular data points out a very high variability between sampled localities, but in some cases also among specimens sampled in the same locality (up to 33.3% p-distance). The integrated approach to the study of Pseudechiniscus allows confirmation of its monophyly and highlights the relationships in the taxon, pointing to its global distribution.

Revision of the little brown frogs in the Gephyromantis decaryi complex with description of a new species

Zootaxa ◽  
2012 ◽  
Vol 3421 (1) ◽  
pp. 32 ◽  
Author(s):  
KATHARINA C. WOLLENBERG ◽  
FRANK GLAW ◽  
MIGUEL VENCES
Keyword(s):  
Mitochondrial Gene ◽  
Short Note ◽  
Distinct Species ◽  
Molecular Data ◽  
Upper Lip ◽  
Candidate Species ◽  
Brown Leaf ◽  
Final Confirmation ◽  
A New Species ◽  
Evolutionary Lineages

We revise the systematics of a group of little brown leaf litter frogs from Madagascar that are notoriously difficult to di-agnose, the Gephyromantis decaryi complex. Using an integrative combination of molecular data, bioacoustics, and mor-phology, we reveal four divergent evolutionary lineages which all are monophyletic in the mitochondrial gene genealogiesand have unique haplotypes in the nuclear RAG1 gene. We recognize three of these as distinct species and a fourth one ascandidate species which requires additional study for final confirmation and description. The three species are (1) G. de-caryi Angel, 1930, from Midongy and Ranomafana, characterized by relatively long limbs and continuous dorsal folds;(2) Gephyromantis verrucosus Angel, 1930 from Vondrozo and Manombo, herein removed from the synonymy of G. bou-lengeri, including the designation of a lectotype, characterized by irregular tubercles on the dorsum, and G. hintelmannaesp. nov. from Tsitolaka forest near Ambohitsara village and a second nearby site, characterized by a relatively smoothdorsum, short note duration in advertisement calls, and lack of light-dark pattern along the upper lip. A fourth lineage mor-phologically similar and phylogenetically placed sister to G. hintelmannae is characterized by the shortest note durationin calls of the complex but we consider the available data as insufficient to fully assess its status and propose to keep considering this lineage as candidate species Gephyromantis sp. 7 until more specimens and data become available.

scholarly journals Thismia gigantea, a new combination in Thismia (Thismiaceae)

Phytotaxa ◽  
2014 ◽  
Vol 172 (1) ◽  
pp. 55
Author(s):  
Michal Hroneš
Keyword(s):  
Species Diversity ◽  
South America ◽  
Molecular Data ◽  
New Combination ◽  
Morphological And Molecular Data ◽  
Evolutionary Lineages

Thismia Griffith (1844: 221; Thismiaceae J. Agardh, 1858: 99) comprises about 55 species (e.g. Tsukaya & Okada 2012; Dančák et al. 2013; Lưu et al. 2014). The greatest species diversity is situated in southeastern Asia and in the northern parts of South America (Merckx et al. 2013). Both morphological and molecular data suggest that the genus is not monophyletic being represented by at least two evolutionary lineages, the neotropical Thismia subg. Ophiomeris (Miers) Maas & H.Maas (1986: 144) and the rest of the species in a broadly defined subg. Thismia (Merckx et al. 2006; Merckx & Smets 2014).

scholarly journals Metabarcoding Advances for Ecology and Biogeography of Neotropical Protists: What Do We Know, Where Do We Go?

2020 ◽  
Author(s):  
Camila Duarte Ritter ◽  
Arielli Fabrício Machado ◽  
Karine Felix Ribeiro ◽  
Micah Dunthorn
Keyword(s):  
Molecular Data ◽  
Neotropical Region ◽  
Research Field ◽  
Microbial World ◽  
Dispersal Abilities ◽  
Taxonomic Groups ◽  
Micro Organisms ◽  
Available Information ◽  
The Relationship ◽  
Evolutionary Lineages

The Neotropical region is one of the most diverse regions of the globe in terms of macro-organismic species. Regarding the microbial world, however, little is known about the diversity and biogeography patterns of micro-organisms in the Neotropics. In this context, the study of several microbial taxonomic groups is still missing and/or incomplete, such as the protists. Our goal here was to summarize the available information of Neotropical protists, focusing on molecular data from environmental continental samples, to explore what these data evidence on their ecology and biogeography. For this, we reviewed the findings from all articles that focused on or included the terrestrial protists using metabarcoding approach and identified the gaps and future perspectives in this research field. We found that Neotropical protists diversity patterns seem to be, at least in part, congruent with that of macro-organisms and, different than plants and bacteria, just weakly explained by environmental variables. We argue that studies with standardized protocols including different biomes are necessary to fully characterize the ecology and biogeography on Neotropical protists. Furthermore, dismember evolutionary lineages and functional guilds of protists are important to better understand the relationship between diversity, dispersal abilities and functionality of particular taxa of protists in their habitats.

Notes on the molecular taxonomy of the Proclossiana eunomia complex (Lepidoptera, Nymphalidae: Argynnini): analysis of DNA barcodes

2018 ◽  
Vol 8 (1) ◽  
pp. 222-232 ◽  
Author(s):  
R. V. Yakovlev ◽  
N. A. Shapoval ◽  
G. N. Kuftina ◽  
A. V. Kulak ◽  
S. V. Kovalev
Keyword(s):  
Body Size ◽  
Molecular Taxonomy ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Molecular Approach ◽  
Dna Barcodes ◽  
Morphological Differences ◽  
Proclossiana Eunomia

The Proclossiana eunomia (Esper, 1799) complex is currently composed of the several subspecies distributed throughout Palaearсtic region and North America. Despite the fact that some of the taxa have differences in wing pattern and body size, previous assumptions on taxonomy not supported by molecular data. Therefore, the identity of certain populations of this complex has remained unclear and the taxonomic status of several recently described taxa is debated. Here, we provide insights into systematics of some Palaearctic members of this group using molecular approach, based on the analysis of the barcoding fragment of the COI gene taking into account known morphological differences.

New Record of a Genus and Species of Odd-Scaled Snake (Serpentes: Xenodermidae) from China

2020 ◽  
Vol 27 (6) ◽  
pp. 348-352
Author(s):  
Cai De Bo ◽  
Zening Chen ◽  
Jun Gao ◽  
Ding Li ◽  
Rong Dai
Keyword(s):  
New Record ◽  
First Record ◽  
Molecular Data ◽  
Extent Of Occurrence ◽  
Morphological And Molecular Data

Parafimbrios has been reported in Laos, Thailand and Vietnam. In this paper, based on morphological and molecular data, Parafimbrios lao was reported as the first record of a genus and species of odd-scaled snake in China. This record extends the extent of occurrence to 65,000 km2 and the area of occurrence to 20 km2.

Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

2019 ◽  
Vol 28 (2) ◽  
pp. 305-316
Author(s):  
M.A. Chursina ◽  
I.Ya. Grichanov
Keyword(s):  
Dna Analysis ◽  
Distinct Species ◽  
Molecular Data ◽  
Wing Shape ◽  
Morphological Data ◽  
12S Rrna ◽  
Separate Species ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis ◽  
Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

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