Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

2019 ◽  
Vol 28 (2) ◽  
pp. 305-316
Author(s):  
M.A. Chursina ◽  
I.Ya. Grichanov
Keyword(s):  
Dna Analysis ◽  
Distinct Species ◽  
Molecular Data ◽  
Wing Shape ◽  
Morphological Data ◽  
12S Rrna ◽  
Separate Species ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis ◽  
Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

Species identity of Macrolophus melanotoma (Costa 1853) and Macrolophus pygmaeus (Rambur 1839) (Insecta: Heteroptera: Miridae) based on morphological and molecular data and bionomic implications

2006 ◽  
Vol 37 (4) ◽  
pp. 385-404 ◽  
Author(s):  
Juan Antonio Sanchez ◽  
Jose Luis Cenis ◽  
Gerasimos Cassis ◽  
Jose Isidro Martinez-Cascales
Keyword(s):  
Phylogenetic Analyses ◽  
Antennal Segment ◽  
Distinct Species ◽  
Molecular Data ◽  
Morphological Data ◽  
Species Identity ◽  
Macrolophus Pygmaeus ◽  
Nesidiocoris Tenuis ◽  
Black Band ◽  
Morphological And Molecular Data

AbstractMacrolophus contains a small group of Palaearctic species with very simple and similar external morphology. The classification of these species has been based on variable characters such as body measurements, the height of the black band-shaped macula behind the eyes and the colour of the first antennal segment. Macrolophus melanotoma (Costa 1853), Macrolophus pygmaeus (Rambur 1839) and Macrolophus costalis Fieber 1858, are the most reputed predators of the genus. The classification history of M. melanotoma and M. pygmaeus shows a great number of misconceptions that have left the identity of the two species in confusion. Despite the economic importance of these two species, they have not received comprehensive taxonomical treatment until now. In this work, the morphological and sequence variation of a cytochrome b fragment (320 bp) were analysed to determine the identity of M. melanotoma and M. pygmaeus. Macrolophus costalis, Dicyphus cerastii Wagner 1951b, Dicyphus tamaninii Wagner 1951b, Cyrtopeltis geniculata Fieber 1861 and Nesidiocoris tenuis (Reuter 1895) were used as outgroup taxons in the phylogenetic analyses. Several evolutionary models were explored under a maximum likelihood framework. Macrolophus melanotoma and M. pygmaeus were classified as two distinct species based on monophyly, molecular and morphological data. Nodes at the species level were supported by high bootstrap values. M. pygmaeus and M. costalis are sister species, M. melanotoma basal to them. The shape of the black macula behind the eye may be used as a diagnostic character to differentiate M. melanotoma from M. pygmaeus with some degree of confidence.

Redescription of Chimaera ogilbyi (Chimaeriformes; Chimaeridae) from the Indo-Australian region

Zootaxa ◽  
2018 ◽  
Vol 4375 (2) ◽  
pp. 191 ◽  
Author(s):  
BRITTANY FINUCCI ◽  
WILLIAM T. WHITE ◽  
JENNY M. KEMPER ◽  
GAVIN J.P. NAYLOR
Keyword(s):  
Papua New Guinea ◽  
Nuclear Dna ◽  
Single Species ◽  
Distinct Species ◽  
Molecular Data ◽  
Morphological Data ◽  
Mitochondrial Markers ◽  
Australian Region ◽  
Taxonomic Approach ◽  
Morphological And Molecular Data

An integrated taxonomic approach, combining both morphological and molecular data, was adopted to investigate the Hydrolagus lemures-ogilbyi group in the Indo-Australian region. Single mitochondrial markers (CO1 and NADH2) provided evidence supporting the separation of four distinct species in this group. However, detailed morphological data collected from specimens from across their range failed to find any consistent differences, and many features previously considered to be diagnostic were found to be variable. Nuclear DNA data also failed to support the differences found with the single mitochondrial markers and, together with the morphological data, supported the hypothesis that only a single species in this group is present in the Indo-Australian region. In addition, the results failed to support the current generic placement of this group in Hydrolagus, suggesting they belong to the genus Chimaera with doubt over the validity of Hydrolagus as a valid genus. The oldest available name for this group is Chimaera ogilbyi and a redescription is provided. This species occurs throughout Australia, eastern Indonesia (Java, Bali, and Lombok) and northern Papua New Guinea. 

scholarly journals Resurrection of Ornithogalum brevipedicellatum (Asparagaceae) with morphological and molecular data

2016 ◽  
Vol 75 (1) ◽  
pp. 60-66
Author(s):  
Candan Aykurt ◽  
İsmail G. Deniz ◽  
Duygu Sari ◽  
Mecit Vural ◽  
Hüseyin Sümbül
Keyword(s):  
Electron Microscopy ◽  
Leaf Morphology ◽  
Distinct Species ◽  
Molecular Data ◽  
Morphological Data ◽  
Diagnostic Characteristics ◽  
Molecular Studies ◽  
Similarities And Differences ◽  
Morphological And Molecular Data ◽  
Scanning Electron

Abstract This study evaluates Ornithogalum brevipedicellatum, which was previously accepted as a synonym of O. oligophyllum, as a separate distinct species and discusses the similarities and differences between O. brevipedicellatum and its related species (O. oligophyllum and O. pamphylicum). Similarities and differences among these species were identifi ed by morphological and molecular studies. The leaf morphology and inflorescence of O. brevipedicellatum and O. pamphylicum are similar to each other, and in terms of these features, they show differences from O. oligophyllum. Some diagnostic characteristics are quite different in O. brevipedicellatum and O. pamphylicum, such as the size of tepals, length of fruiting pedicels and style. Morphological data were supported by the results obtained from molecular studies. According to a dendrogram obtained by molecular studies, O. brevipedicellatum and O. pamphylicum are similar. O. oligophyllum is more closely related to O. pyrenaicum used as an out-group. Additionally, the seeds of O. brevipedicellatum were examined with the use of scanning electron microscopy

scholarly journals Silene sunhangii (Caryophyllaceae), a new species from China

PhytoKeys ◽  
2019 ◽  
Vol 135 ◽  
pp. 59-69
Author(s):  
Nan Lin ◽  
Dai-Gui Zhang ◽  
Xian-Han Huang ◽  
Jian-Wen Zhang ◽  
Jing-Yuan Yang ◽  
...  
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Its Region ◽  
Distinct Species ◽  
Molecular Data ◽  
Central China ◽  
Morphological Data ◽  
Nuclear Its ◽  
Morphological And Molecular Data ◽  
A New Species

Silene sunhangii, a new species of Caryophyllaceae known from only three populations in Hubei and Hunan provinces of central China, is described. Both morphological and molecular data were used to assess the taxonomic status and relationships of this species. Morphologically, S. sunhangii is most similar to S. platyphylla Franch. from which it differs most readily in having 3-veined elliptical leaves without pubescence, tasseled catacorolla, pale purple to red petals without a linear lobe or narrow tooth and lanceolate, bifid to one third. A phylogenetic analysis based on nuclear ITS region identified the new species as a well-supported, independent lineage. Our new species is nested within a grade that encompasses species representing a polyphyletic Silene sect. Physolychnis (Benth.) Bocquet. Both the genetic and morphological data support the recognition of Silene sunhangii as a distinct species, although there is inconsistency between these two datasets as to the relationships of the new species.

scholarly journals A NEW SPECIES OF THE Pareas hamptoni COMPLEX (SQUAMATA, SERPENTES, PAREIDAE) FROM THE GOLDEN TRIANGLE

2020 ◽  
pp. 174-193
Author(s):  
Li Ding ◽  
Zening Chen ◽  
Chatmongkon Suwannapoom ◽  
Tan Van Nguyen ◽  
Nikolay A. Poyarkov ◽  
...  
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Southern China ◽  
Taxonomic Status ◽  
Distinct Species ◽  
Molecular Data ◽  
Lower Number ◽  
Actual Distribution ◽  
Golden Triangle ◽  
Morphological And Molecular Data

An investigation of the taxonomic status of Pareas hamptoni (Hampton's Slug snake) based on morphological and molecular data revealed a new distinct species from the Golden Triangle region (comprising parts of southern China, and adjacent Laos and Thailand). The new species is shown to be a sister species to P. hamptoni but can be separated from the latter by having 3–5 dorsal scale rows at midbody slightly keeled (vs 5–9 scales strongly keeled); a lower number of ventrals, 170–188 (vs 185–195); and a lower number of subcaudals, 67–91 (vs 91–99). The new species is currently known from northwestern Thailand, northern Laos, and the southern part of Yunnan Province in China at elevations of 1,160–2,280 m a.s.l. We suggest that the new species to be considered of Least Concern (LC) in the IUCN‘s Red List categories. Problems of taxonomy and actual distribution of the P. hamptoni complex are briefly discussed; our results show P. hamptoni is now reliably known only from Myanmar and Vietnam, but its occurrence in Yunnan Province of China is likely.

scholarly journals New free-living nematode species and records (Chromadorea: Plectida and Desmodorida) from the edge and axis of Kermadec Trench, Southwest Pacific Ocean

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12037
Author(s):  
Daniel Leduc
Keyword(s):  
Nematode Species ◽  
Distinct Species ◽  
Abundant Species ◽  
Molecular Data ◽  
Weddell Sea ◽  
Body Contour ◽  
Southwest Pacific ◽  
Free Living Nematode ◽  
Strong Resemblance ◽  
Morphological And Molecular Data

One new nematode species is described and two new species records are provided from the edge (6,080 m depth) and axis (7,132 m) of Kermadec Trench, Southwest Pacific. Leptolaimus hadalis sp. nov. is characterised by medium body 587–741 μm long, labial region not offset from body contour, inconspicuous labial sensilla, amphid located 12–19 μm from anterior end, female without supplements, male with four tubular precloacal supplements (alveolar supplements absent), tubular supplements almost straight with dentate tip, arcuate spicules and weakly cuticularized dorsal gubernacular apophyses strongly bent distally. In a previously published ecological survey of Kermadec Trench, L. hadalis sp. nov. was the most abundant species in a core obtained at 8,079 m water depth and third most abundant species in a core obtained at 7,132 m, while only one individual was found at 6,096 m depth, and none at 9,175 m depth (Leduc & Rowden, 2018). Alaimella aff. cincta and Desmodora aff. pilosa are recorded for the first time from the Southwest Pacific region. Prior to the present study, Alaimella had only been recorded from coastal locations and from the Weddell sea to a depth of 2,000 m. The record of Desmodora aff. pilosa at 6,080 m depth is the deepest record of a Desmodora species to date, although unidentified Desmodora specimens have been found as deep as 6,300 m in the South Sandwich Trench. The morphology of the Kermadec Trench Alaimella aff. cincta and Desmodora aff. pilosa specimens bear a strong resemblance to their respective type populations from the Northern Hemisphere, but further morphological and molecular data are required to ascertain whether they in fact represent distinct species.

scholarly journals The hidden anatomy of paranasal sinuses reveals biogeographically distinct morphotypes in the nine-banded armadillo (Dasypus novemcinctus)

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3593 ◽  
Author(s):  
Guillaume Billet ◽  
Lionel Hautier ◽  
Benoit de Thoisy ◽  
Frédéric Delsuc
Keyword(s):  
South America ◽  
Paranasal Sinuses ◽  
Morphological Diversity ◽  
Distinct Species ◽  
Molecular Data ◽  
Morphological Data ◽  
Diagnostic Feature ◽  
Guiana Shield ◽  
Diagnostic Features ◽  
Continental Scale

BackgroundWith their Pan-American distribution, long-nosed armadillos (genusDasypus) constitute an understudied model for Neotropical biogeography. This genus currently comprises seven recognized species, the nine-banded armadillo (D. novemcinctus) having the widest distribution ranging from Northern Argentina to the South-Eastern US. With their broad diversity of habitats, nine-banded armadillos provide a useful model to explore the effects of climatic and biogeographic events on morphological diversity at a continental scale.MethodsBased on a sample of 136 skulls ofDasypusspp. belonging to six species, including 112 specimens identified asD. novemcinctus, we studied the diversity and pattern of variation of paranasal cavities, which were reconstructed virtually using µCT-scanning or observed through bone transparency.ResultsOur qualitative analyses of paranasal sinuses and recesses successfully retrieved a taxonomic differentiation between the traditional speciesD. kappleri,D. pilosusandD. novemcinctusbut failed to recover diagnostic features between the disputed and morphologically similarD. septemcinctusandD. hybridus. Most interestingly, the high variation detected in our large sample ofD. novemcinctusshowed a clear geographical patterning, with the recognition of three well-separated morphotypes: one ranging from North and Central America and parts of northern South America west of the Andes, one distributed across the Amazonian Basin and central South America, and one restricted to the Guiana Shield.DiscussionThe question as to whether these paranasal morphotypes may represent previously unrecognized species is to be evaluated through a thorough revision of theDasypusspecies complex integrating molecular and morphological data. Remarkably, our recognition of a distinct morphotype in the Guiana Shield area is congruent with the recent discovery of a divergent mitogenomic lineage in French Guiana. The inflation of the second medialmost pair of caudal frontal sinuses constitutes an unexpected morphological diagnostic feature for this potentially distinct species. Our results demonstrate the benefits of studying overlooked internal morphological structures in supposedly cryptic species revealed by molecular data. It also illustrates the under-exploited potential of the highly variable paranasal sinuses of armadillos for systematic studies.

Revision of Autolytinae (Syllidae: Polychaeta)

Zootaxa ◽  
2004 ◽  
Vol 680 (1) ◽  
pp. 1 ◽  
Author(s):  
ARNE NYGREN
Keyword(s):  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Nominal Species ◽  
Strict Consensus Tree ◽  
Incertae Sedis ◽  
Nomina Dubia ◽  
Morphological And Molecular Data ◽  
Combined Data

Autolytinae is revised based on available types, and newly collected specimens. Out of 170 nominal species, 18 are considered as incertae sedis, 43 are regarded as junior synonyms, and 25 are referred to as nomina dubia. The relationships of Autolytinae is assessed from 51 morphological characters and 211 states for 76 ingroup-taxa, and 460 molecular characters from mitochondrial 16S rDNA and nuclear 18S rDNA for 31 ingroup-taxa; outgroups include 12 non-autolytine syllid polychaetes. Two analyses are provided, one including morphological data only, and one with combined morphological and molecular data sets. The resulting strict consensus tree from the combined data is chosen for a reclassification. Three main clades are identified: Procerini trib. n., Autolytini Grube, 1850, and Epigamia gen. n. Proceraea Ehlers, 1864 and Myrianida Milne Edwards, 1845 are referred to as nomen protectum, while Scolopendra Slabber, 1781, Podonereis Blainville, 1818, Amytis Savigny, 1822, Polynice Savigny, 1822, and Nereisyllis Blainville, 1828 are considered

A molecular phylogeny of Callianassidae and related families (Crustacea : Decapoda : Axiidea) with morphological support

2020 ◽  
Vol 34 (2) ◽  
pp. 113 ◽  
Author(s):  
Rafael Robles ◽  
Peter C. Dworschak ◽  
Darryl L. Felder ◽  
Gary C. B. Poore ◽  
Fernando L. Mantelatto
Keyword(s):  
Bayesian Analysis ◽  
Molecular Analysis ◽  
Single Species ◽  
Molecular Data ◽  
Total Evidence ◽  
Morphological Data ◽  
12S Rrna ◽  
New Genera ◽  
Key Species ◽  
The Family

The axiidean families Callianassidae and Ctenochelidae, sometimes treated together as Callianassoidea, are shown to represent a monophyletic taxon. It comprises 265 accepted species in 74 genera, twice this number of species if fossil taxa are included. The higher taxonomy of the group has proved difficult and fluid. In a molecular phylogenetic approach, we inferred evolutionary relationships from a maximum-likelihood (ML) and Bayesian analysis of four genes, mitochondrial 16S rRNA and 12S rRNA along with nuclear histone H3 and 18S rRNA. Our sample consisted of 298 specimens representing 123 species plus two species each of Axiidae and Callianideidae serving as outgroups. This number represented about half of all known species, but included 26 species undescribed or not confidently identified, 9% of all known. In a parallel morphological approach, the published descriptions of all species were examined and detailed observations made on about two-thirds of the known fauna in museum collections. A DELTA (Description Language for Taxonomy), database of 135 characters was made for 195 putative species, 18 of which were undescribed. A PAUP analysis found small clades coincident with the terminal clades found in the molecular treatment. Bayesian analysis of a total-evidence dataset combined elements of both molecular and morphological analyses. Clades were interpreted as seven families and 53 genera. Seventeen new genera are required to reflect the molecular and morphological phylograms. Relationships between the families and genera inferred from the two analyses differed between the two strategies in spite of retrospective searches for morphological features supporting intermediate clades. The family Ctenochelidae was recovered in both analyses but the monophyly of Paragourretia was not supported by molecular data. The hitherto well recognised family Eucalliacidae was found to be polyphyletic in the molecular analysis, but the family and its genera were well defined by morphological synapomorphies. The phylogram for Callianassidae suggested the isolation of several species from the genera to which they had traditionally been assigned and necessitated 12 new generic names. The same was true for Callichiridae, with stronger ML than Bayesian support, and five new genera are proposed. Morphological data did not reliably reflect generic relationships inferred from the molecular analysis though they did diagnose terminal taxa treated as genera. We conclude that discrepancies between molecular and morphological analyses are due at least in part to missing sequences for key species, but no less to our inability to recognise unambiguously informative morphological synapomorphies. The ML analysis revealed the presence of at least 10 complexes wherein 2–4 cryptic species masquerade under single species names.

Morphological and genetic evidence of the full species status of Iberolacerta cyreni martinezricai (Arribas, 1996)

Zootaxa ◽  
2004 ◽  
Vol 634 (1) ◽  
pp. 1 ◽  
Author(s):  
Oscar Arribas ◽  
Salvador Carranza
Keyword(s):  
Nuclear Gene ◽  
Mediterranean Area ◽  
Molecular Data ◽  
Climatic Conditions ◽  
Morphological Data ◽  
Distribution Area ◽  
Alternative Hypotheses ◽  
The Mediterranean ◽  
Level I ◽  
Morphological And Molecular Data

Iberolacerta cyreni martinezricai is elevated to the species level (I. martinezricai) based on both morphological and molecular data. The phylogenetic analysis using two mitochondrial and one nuclear gene shows I. martinezricai is more closely related to I. monticola than to I. cyreni. A multivariate analysis of the morphological data also supports the affinities between I. martinezricai and I. monticola but, at the same time, clearly indicates that I. martinezricai is morphologically distinct from both I. monticola and I. cyreni. The molecular data suggests I. cyreni and the clade formed by I. monticola + I. martinezricai split approximately 6.1 Mya, during the Mesinian Salinity Crisis, when climatic conditions around the Mediterranean area changed dramatically as a result of the desiccation of the Mediterranean Sea. Separation between I. martinezricai and I. monticola occurred approximately 2 Mya but, with at least two equally plausible alternative hypotheses, their biogeography is still unclear. New data on the habitat and distribution of I. martinezricai indicates its distribution area is very small (12 15 km 2 ), and that it lives in a climatically extreme habitat for this kind of mountain species. As a result of that and the low numbers of individuals, I. martinezricai is considered here as Critically Endangered.

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