scholarly journals 657. Combination Therapy with Cyclophosphamide and the Oncolytic Herpes Virus Mutant rRp450, Expressing a Cyclophosphamide Prodrug, Is Clinically Safe by Intratumoral, Intravenous, and Intracerebral Administration in an HSV-1 Susceptible Mouse Model

2006 ◽  
Vol 13 ◽  
pp. S253
Author(s):  
Mark A. Currier ◽  
Yonatan Y. Mahller ◽  
Greg Stroup ◽  
Hirokazu Kambara ◽  
E.A. Chiocca ◽  
...  
Keyword(s):  
Combination Therapy ◽  
Mouse Model ◽  
Herpes Virus ◽  
Susceptible Mouse ◽  
Intracerebral Administration ◽  
Herpès Virus ◽  
Virus Mutant ◽  
Hsv 1

scholarly journals Frequency of Herpes Virus (HSV) in Esophageal Muscle Samples among Patients with Achalasia under Heller Myotomy

2021 ◽  
Author(s):  
Tahmaseb Jouzdani ◽  
Amir Sadeghi ◽  
Hamed Tahmasbi ◽  
Ramin Shekouhi ◽  
Maryam Sohooli ◽  
...  
Keyword(s):  
Herpes Virus ◽  
Viral Agent ◽  
Heller’S Myotomy ◽  
History Of ◽  
Heller's Myotomy ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Herpès Virus ◽  
Esophageal Muscle ◽  
Hsv 1

Abstract Background Despite years of research, the etiology of achalasia not well understood. Scientists suppose a role for autoimmunity, in this disorder, and probable viral agent, such as herpes virus (HSV). The aim was to find out the frequency of HSV in esophageal muscle samples in patients with achalasia under Heller's myotomy. Methods In this study, 60 patients with achalasia, after fulfilling the consent form, were underwent Heller’s myotomy surgery. Biopsy samples prepared for polymerase chain reaction (PCR) method for HSV DNA detection. After DNA-extraction, replication performed using specific primers. Results The mean age was 40.62 ± 5.08 years. Thirty-nine patients (65%) were female and 21 (35%) were male. Thirty-eight (63.3%) had no history but the else (36.7%) had a positive history of HSV. HSV-1 was positive in three patients (5%). Two females and one male were HSV-positive. Conclusions HSV-1 frequency is not notable among Iranian patients with achalasia. We suggest exploring other viruses, in special that involving the pathogenesis of achalasia, with a larger sample size.

scholarly journals Pathological processes of immunological decontamination of herpes simplex virus type 1 from the cornea

2020 ◽  
pp. 4-11
Author(s):  
Т.З. Керимов ◽  
В.П. Соболев ◽  
М.А. Соболева ◽  
Н.А. Гаврилова ◽  
С.А. Борзенок
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Herpes Virus ◽  
Toll Like Receptors ◽  
Herpès Virus ◽  
Simplex Virus ◽  
Hsv 1

В обзоре представлено описание патофизиологических механизмов герпесвирусной инфекции. Согласно данным медицинской статистики, вирусом простого герпеса 1 типа инфицировано большинство населения планеты. В развивающихся странах данный вирус является ведущей инфекционной причиной поражения роговицы. Также вирусу простого герпеса 1 типа отводится роль одного из факторов, приводящих к отторжению трансплантата роговицы. Вышеописанные патологические явления сопряжены с перестройкой клеточных систем в ответ на вирусное воздействие. Недавние открытия в данной области обнаружили значительный вклад трансмембранных и эндосомальных Toll-подобных рецепторов во врожденный противовирусный клеточный ответ. Показано, что эндосомальные Toll-подобные рецепторы 3 типа экспрессируются в кератоцитах только после их фенотипического перехода в фибробласты. Данная трансформация обычно происходит в результате механических и патогенных воздействий на роговицу. Изменение рецепторного состава клеток в ответ на герпесвирусную инвазию вызывает выработку интерферонов 1 типа - интерферона-альфа, интерферона-бета, и синтезу провоспалительных цитокинов, что приводит к вирусной деконтаминации. This review describes pathophysiological mechanisms of herpes virus infection in cornea cells. It has been previously reported that herpes simplex virus type 1 (HSV-1) infects most of the world’s population. In developing countries, HSV-1 is the leading infectious cause of corneal damage. Also, herpes simplex virus type 1 was assigned the role of one of the factors leading to rejection of the corneal transplant. These pathological phenomena are associated with restructuring of cellular systems in response to viral exposure. Recent discoveries have revealed a significant contribution of transmembrane and endosomal Toll-like receptors to the innate antiviral cell response. It is well known that endosomal Toll-like receptors-3 are expressed in keratocytes only after their phenotypic transformation to fibroblasts. This transformation usually occurs as a result of mechanical or infectious impact on the cornea. Changes in the receptor composition of cells as a response to herpes virus invasion is the main cause of type 1 interferons (interferon-alpha and interferon-beta) production and expression of proinflammatory cytokines, which leads to viral decontamination.

Lithium resistant herpes virus mutant

1991 ◽  
Vol 15 ◽  
pp. 110
Author(s):  
S Randall ◽  
C.E Hartley ◽  
A Buchan ◽  
G.R.B Skinner
Keyword(s):  
Herpes Virus ◽  
Herpès Virus ◽  
Virus Mutant

Efficacy of herpes virus helicase-primase inhibitor, ASP2151, for treating herpes simplex keratitis in mouse model

2013 ◽  
Vol 97 (4) ◽  
pp. 498-503 ◽  
Author(s):  
Shin-Ichi Sasaki ◽  
Dai Miyazaki ◽  
Tomoko Haruki ◽  
Yukimi Yamamoto ◽  
Michiko Kandori ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Mouse Model ◽  
Herpes Virus ◽  
Herpes Simplex Keratitis ◽  
Herpès Virus

scholarly journals Clinical, neuropathological, and immunological short‐ and long‐term feature of a mouse model mimicking human herpes virus encephalitis

2021 ◽  
Author(s):  
Julia Sehl‐Ewert ◽  
Theresa Schwaiger ◽  
Alexander Schäfer ◽  
Julia E. Hölper ◽  
Barbara G. Klupp ◽  
...  
Keyword(s):  
Mouse Model ◽  
Herpes Virus ◽  
Human Herpes ◽  
Virus Encephalitis ◽  
Human Herpes Virus ◽  
Short And Long Term ◽  
Herpès Virus

scholarly journals Characterization of heterosubunit complexes formed by the R1 and R2 subunits of herpes simplex virus 1 and equine herpes virus 4 ribonucleotide reductase

2000 ◽  
Vol 347 (1) ◽  
pp. 97-104
Author(s):  
Yunming SUN ◽  
Joe CONNER
Keyword(s):  
Enzyme Activity ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Ribonucleotide Reductase ◽  
Herpes Virus ◽  
Subunit Interaction ◽  
Herpes Simplex Virus 1 ◽  
Herpès Virus ◽  
Simplex Virus ◽  
Hsv 1

We report on the separate PCR cloning and subsequent expression and purification of the large (R1) and small (R2) subunits from equine herpes virus type 4 (EHV-4) ribonucleotide reductase. The EHV-4 R1 and R2 subunits reconstituted an active enzyme and their abilities to complement the R1 and R2 subunits from the closely related herpes simplex virus 1 (HSV-1) ribonucleotide reductase, with the use of subunit interaction and enzyme activity assays, were analysed. Both EHV-4 R1/HSV-1 R2 and HSV-1 R1/EHV-4 R2 were able to assemble heterosubunit complexes but, surprisingly, neither of these complexes was fully active in enzyme activity assays; the EHV-4 R1/HSV-1 R2 and HSV-1 R1/EHV-4 R2 enzymes had 50% and 5% of their respective wild-type activities. Site-directed mutagenesis was used to alter two non-conserved residues located within the highly conserved and functionally important C-termini of the EHV-4 and HSV-1 R1 proteins. Mutation of Pro-737 to Lys and Lys-1084 to Pro in EHV-4 and HSV-1 R1 respectively had no effects on subunit assembly. Mutation of Pro-737 to Lys in EHV-4 R1 decreased enzyme activity by 50%; replacement of Lys-1084 by Pro in HSV-1 R1 had no effect on enzyme activity. Both alterations failed to restore full enzyme activities to the heterosubunit enzymes. Therefore probably neither of these amino acids has a direct role in catalysis. However, mutation of the highly conserved Tyr-1111 to Phe in HSV-1 R1 inactivated enzyme activity without affecting subunit interaction.

Signaling Pathway Used by HSV-1 to Induce NF- B Activation: Possible Role of Herpes Virus Entry Receptor A

2007 ◽  
Vol 1096 (1) ◽  
pp. 89-96 ◽  
Author(s):  
M. TERESA SCIORTINO ◽  
M. ANTONIETTA MEDICI ◽  
F. MARINO-MERLO ◽  
D. ZACCARIA ◽  
M. GIUFFRE ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Herpes Virus ◽  
Virus Entry ◽  
Herpès Virus ◽  
Entry Receptor ◽  
Hsv 1

scholarly journals Influence of the immunomodulatory drug stimforte on the humoral immune response in the experimental herpes virus infection

2016 ◽  
Vol 61 (4) ◽  
pp. 172-175
Author(s):  
D. G. Maldov ◽  
V. L. Andronova ◽  
A. A. Balakina ◽  
A. V. Ilyichev ◽  
G. A. Galegov
Keyword(s):  
Virus Infection ◽  
Herpes Virus ◽  
Immune Memory ◽  
Dot Blot ◽  
Herpes Virus Infection ◽  
Western Blots ◽  
Humoral Immune ◽  
Herpès Virus ◽  
Dot Blot Analysis ◽  
Hsv 1

In the study of the immunostimulation preparation Stimforte activity using the model of the experimental herpes virus infection BALB/c, mice has shown that sera from mice treated with the drug on the 4th and 7th day after infection possessed a 3 times greater capability of specifically binding to the culture of HSV-1 (on cells Vero) according to dot blot analysis, as compared with intact infected mice sera obtained at the same time. It was also shown that these sera had a 5 times higher index of neutralization. On the basis of Western blots, it was detected that antibodies from sera of mice treated with Stimforte contacted the glycoproteins gB and gC of HSV-1 significantly better. Thus, Stimforte stimulates one of the strongest modulatory effects on the immune memory and is a promising drug for the treatment of chronic viral diseases.

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