High level expression of porcine growth hormone in Escherichia coli from an expression vector containing bacteriophage λ P and N gene untranslated region

1990 ◽  
Vol 173 (2) ◽  
pp. 711-717 ◽  
Author(s):  
Lih-Hwa Hwang ◽  
Huei-Feng Tsi ◽  
Shih-Tung Liu
Keyword(s):  
Escherichia Coli ◽  
Growth Hormone ◽  
Untranslated Region ◽  
Expression Vector ◽  
N Gene ◽  
Bacteriophage Λ ◽  
High Level Expression ◽  
High Level

High–Level Expression, Efficient Secretion and Folding of Human Growth Hormone in Escherichia coli

1986 ◽  
Vol 4 (11) ◽  
pp. 991-995 ◽  
Author(s):  
Hansen M. Hsiung ◽  
Nancy G. Mayne ◽  
Gerald W. Becker
Keyword(s):  
Escherichia Coli ◽  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
High Level Expression ◽  
High Level

scholarly journals Importance of a 5′ Stem-Loop for Longevity ofpapA mRNA in Escherichia coli

1999 ◽  
Vol 181 (11) ◽  
pp. 3587-3590 ◽  
Author(s):  
Angela L. Bricker ◽  
Joel G. Belasco
Keyword(s):  
Escherichia Coli ◽  
Untranslated Region ◽  
High Level Expression ◽  
Large Measure ◽  
Stem Loop ◽  
Stem Loop Structure ◽  
Significant Acceleration ◽  
Long Lifetime ◽  
High Level

ABSTRACT High-level expression of the major pilus subunit (PapA) of uropathogenic strains of Escherichia coli results in part from the unusually long lifetime of the mRNA that encodes this protein. Here we report that the longevity of papA mRNA derives in large measure from the protection afforded by its 5′ untranslated region. This papA RNA segment can prolong the lifetime of an otherwise short-lived mRNA to which it is fused. In vivo alkylation studies indicate that, in its natural milieu, the papAmessage begins with a stem-loop structure. This stem-loop is important for the stabilizing effect of the papA 5′ untranslated region, as evidenced by the significant acceleration inpapA mRNA decay that results from its removal.

Phylogeny and expression of the nucleocapsid gene of porcine epidemic diarrhoea virus

2013 ◽  
Vol 61 (2) ◽  
pp. 257-269 ◽  
Author(s):  
Wei Yang ◽  
Guangxing Li ◽  
Yudong Ren ◽  
Siqingaowa Suo ◽  
Xiaofeng Ren
Keyword(s):  
Escherichia Coli ◽  
Protein Sequences ◽  
N Gene ◽  
Severe Diarrhoea ◽  
High Level Expression ◽  
Nucleocapsid Gene ◽  
N Protein ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
High Level

Porcine epidemic diarrhoea virus (PEDV) is one of the important pathogens that may cause severe diarrhoea in piglets. In this study, the nucleocapsid (N) gene of a Chinese PEDV isolate designated HLJBY was cloned. The phylogeny of PEDV strains was investigated by constructing a phylogenetic tree based on the N protein sequences. The results indicate that there are two major groups of Chinese PEDVs, a Japanese PEDV group and a Korean PEDV group. High-level expression of the N protein was achieved inEscherichia coli. The immunoreactivity between PEDV particles or the bacterially expressed N protein and rabbit anti-PEDV serum was confirmed by immunofluorescence assays and Western blot. Both PEDV N protein and the polyclonal antibody generated in this study are valuable diagnostic reagents for PEDV surveillance.

A tightly regulated high level expression vector that utilizes a thermosensitive lac repressor: production of the human T cell receptor Vβ5.3 in Escherichia coli

Gene ◽  
1996 ◽  
Vol 182 (1-2) ◽  
pp. 101-109 ◽  
Author(s):  
Beth Andrews ◽  
Hedy Adari ◽  
Gerhard Hannig ◽  
Elaine Lahue ◽  
Michael Gosselin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
T Cell ◽  
T Cell Receptor ◽  
Expression Vector ◽  
Cell Receptor ◽  
Lac Repressor ◽  
High Level Expression ◽  
Human T Cell ◽  
High Level
Sign in / Sign up

Export Citation Format

Share Document