Microinjection of vesicular stomatitis virus ribonucleoprotein into animal cells yields infectious virus

1983 ◽  
Vol 116 (3) ◽  
pp. 1160-1167 ◽  
Author(s):  
George B. Thornton ◽  
John J. Kopchick ◽  
Dennis W. Stacey ◽  
Amiya K. Banerjee
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Infectious Virus ◽  
Animal Cells ◽  
Vesicular Stomatitis

scholarly journals Comparison of the Efficacy of Disinfectant Pre-impregnated Wipes for Decontaminating Stainless Steel Carriers Experimentally Inoculated With Ebola Virus and Vesicular Stomatitis Virus

2021 ◽  
Vol 9 ◽  
Author(s):  
Todd A. Cutts ◽  
Samantha B. Kasloff ◽  
Jay Krishnan ◽  
Raymond W. Nims ◽  
Steven S. Theriault ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Ebola Virus ◽  
Infectious Virus ◽  
Negative Control ◽  
Surface Cleaning ◽  
Ammonium Compound ◽  
Virus Transfer ◽  
Vesicular Stomatitis ◽  
Secondary Carriers ◽  
Titration Assay

The authors evaluated four disinfectant pre-impregnated wipes (DPW) for efficacy against Ebola virus Makona variant (EBOV) and vesicular stomatitis virus (VSV), Indiana serotype. Steel carriers were inoculated with the infectious virus and then were wiped with DPW in the Wiperator instrument per ASTM E2967-15. Following the use of J-Cloth impregnated with medium (negative control wipes) or the use of activated hydrogen peroxide (AHP)-, ethanol-, sodium hypochlorite (NaOCl)-, or single or dual quaternary ammonium compound (QAC)-based DPW, virus recovery from the carriers was assayed by titration assay and by two passages on Vero E6 cells in 6-well plates. The Wiperator also enabled the measurement of potential transfer of the virus from the inoculated carrier to a secondary carrier by the DPW or control wipes. The J-Cloth wipes wetted with medium alone (no microbicidal active) removed 1.9–3.5 log10 of virus from inoculated carriers but transferred ~4 log10 of the wiped virus to secondary carriers. DPW containing AHP, ethanol, NaOCl, or single or dual QAC as active microbicidal ingredients removed/inactivated ~6 log10 of the virus, with minimal EBOV or no VSV virus transfer to a secondary surface observed. In Ebola virus outbreaks, a DPW with demonstrated virucidal efficacy, used as directed, may help to mitigate the unintended spread of the infectious virus while performing surface cleaning.

Comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and L cells

1976 ◽  
Vol 22 (10) ◽  
pp. 1458-1463 ◽  
Author(s):  
Kathleen N. Potter ◽  
Robert B. Stewart
Keyword(s):  
Chick Embryo ◽  
Vesicular Stomatitis Virus ◽  
Virus Replication ◽  
Infectious Virus ◽  
Particle Synthesis ◽  
Infectious Particle ◽  
L Cells ◽  
Vesicular Stomatitis ◽  
Defective Interfering Particle

A comparison of the ability of vesicular stomatitis virus (VSV) to generate and replicate defective interfering (DI) particles in primary chick embryo (CE) and mouse L cells was investigated as a means of analyzing host control over DI-particle synthesis and interfering capacity. Serial undiluted passage of VSV in CE and L cells indicate that VSV-DI particles are generated and (or) replicate with greater efficiency in CE than in L cells. When DI particles accumulate in L cells, they are able to interfere with infectious particle replication. The DI particles from CE cells interfered to the same extent with infectious particle replication in both CE and L cells. L cells, therefore, are not considered 'low-interference' hosts in which DI particles are produced and do not interfere with infectious virus replication, but rather hosts which restrict the production of DI particles.

626: Initial Experience with Vesicular Stomatitis Virus as a Treatment for Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 202-202
Author(s):  
Sherwin Zargaroff ◽  
Yuancheng Wang ◽  
Xiayong Zheng ◽  
Jian Pu ◽  
Savio L. Woo ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Vesicular Stomatitis Virus ◽  
Initial Experience ◽  
Vesicular Stomatitis

Inhibition of Various Steps in the Replication Cycle of Vesicular Stomatitis Virus Contributes to Its Photoinactivation by AlPcS4 or Pc4 and Red Light

1999 ◽  
Vol 69 (3) ◽  
pp. 353 ◽  
Author(s):  
Anne C. E. Moor ◽  
Angeline E. Wagenaars-van Gompel ◽  
Ralph C. A. Hermanns ◽  
Jannes van der Meulen ◽  
Jolanda Smit ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Red Light ◽  
Replication Cycle ◽  
Vesicular Stomatitis
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