Inhibitory effect of YC-1 on the hypoxic induction of erythropoietin and vascular endothelial growth factor in Hep3B cells11Abbreviations: EPO, erythropoietin; VEGF, vascular endothelial growth factor; HIF, hypoxia-inducible factor; NO, nitric oxide; NOS, nitric oxide synthase; CO, carbon monoxide; sGC, soluble guanylate cyclase; cGMP, cyclic GMP; YC-1, 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole; SNP, sodium nitroprusside; ODQ, 1H-(1,2,4)oxadiazole (4,3a)quinoxatin-1-one; MB, methylene blue; NAME, N(G)-nitro-L-arginine methyl ester; RT-PCR, reverse transcription polymerase chain reaction; and EMSA, electrophoretic mobility gel shift.

2001 ◽  
Vol 61 (8) ◽  
pp. 947-954 ◽  
Author(s):  
Yang-Sook Chun ◽  
Eun-Jin Yeo ◽  
Eunjoo Choi ◽  
Che-Ming Teng ◽  
Jae-Moon Bae ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Soluble Guanylate Cyclase ◽  
Hypoxia Inducible Factor ◽  
Inhibitory Effect ◽  
Vascular Endothelial ◽  
Polymerase Chain ◽  
Oxide Synthase ◽  
Endothelial Growth Factor

Hypoxia response element of the human vascular endothelial growth factor gene mediates transcriptional regulation by nitric oxide: control of hypoxia-inducible factor-1 activity by nitric oxide

Blood ◽  
2000 ◽  
Vol 95 (1) ◽  
pp. 189-197 ◽  
Author(s):  
Hideo Kimura ◽  
Alessandro Weisz ◽  
Yukiko Kurashima ◽  
Kouichi Hashimoto ◽  
Tsutomu Ogura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Hypoxia Inducible Factor ◽  
Vascular Endothelial ◽  
Hypoxia Response Element ◽  
Response Element ◽  
Hypoxia Response ◽  
Hypoxia Inducible Factor 1 ◽  
Endothelial Growth Factor

Abstract Nitric oxide (NO) regulates production of vascular endothelial growth factor (VEGF) by normal and transformed cells. We demonstrate that NO donors may up-regulate the activity of the human VEGF promoter in normoxic human glioblastoma and hepatoma cells independent of a cyclic guanosine monophosphate–mediated pathway. Deletion and mutation analysis of the VEGF promoter indicates that the NO-responsive cis-elements are the hypoxia-inducible factor-1 (HIF-1) binding site and an adjacent ancillary sequence that is located immediately downstream within the hypoxia-response element (HRE). This work demonstrates that the HRE of this promoter is the primary target of NO. In addition, VEGF gene regulation by NO, as well as by hypoxia, is potentiated by the AP-1 element of the gene. Our study also reveals that NO and hypoxia induce an increase in HIF-1 binding activity and HIF-1 protein levels, both in the nucleus and the whole cell. These results suggest that there are common features of the NO and hypoxic pathways of VEGF induction, while in part, NO mediates gene transcription by a mechanism distinct from hypoxia. This is demonstrated by a difference in sensitivity to guanylate cyclase inhibitors and a different pattern of HIF-1 binding. These results show that there is a primary role for NO in the control of VEGF synthesis and in cell adaptations to hypoxia. (Blood. 2000;95:189-197)

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