The in vivo uptake of l-[I-14C]ascorbic acid by the rat retina and adrenal gland

Abstract. Effects of deficiency in ascorbic acid on in vivo production of corticosterone and testosterone were examined using a mutant strain of rats unable to synthesize ascorbic acid. The adrenal weight of scorbutic rats was larger, and corticosterone levels in plasma and adrenal tissues were higher than those of ascorbic acid-supplied (ascorbutic) rats. Acute and chronic stimulation with ACTH increased corticosterone levels in both ascorbutic and scorbutic rats. In contrast, weights of seminal vesicles and ventral prostates in unstimulated scorbutic rats were smaller, and testosterone levels in plasma and testicular tissues were lower than those in ascorbutic rats. Acute stimulation with hCG increased testosterone levels only slightly in plasma and not in testicular tissues of scorbutic rats, when testosterone levels in ascorbutic rats reached a maximum. Chronic stimulation with hCG increased testosterone levels remarkably in both ascorbutic and scorbutic rats. These findings seem to indicate that ascorbic acid is not essential for the synthesis of steroid hormones. The scurvy seems to increase plasma ACTH levels secondary to the stress, resulting in the stimulation of the adrenals. In contrast, a prolonged deficiency in ascorbic acid appears to decrease plasma gonadotropin levels, and may reduce the sensitivity of testes to gonadotropins.

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In vitro and in vivo reduction of erythrocyte sorbitol by ascorbic acid

Diabetes ◽

10.2337/diabetes.38.8.1036 ◽

1989 ◽

Vol 38 (8) ◽

pp. 1036-1041 ◽

Cited By ~ 15

Author(s):

J. A. Vinson ◽

M. E. Staretz ◽

P. Bose ◽

H. M. Kassm ◽

B. S. Basalyga

Keyword(s):

Ascorbic Acid

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In vivo uptake and retention of fluoride after a brief application of TiF4 to dentin

Acta Odontologica Scandinavica ◽

10.3109/00016358909004720 ◽

1989 ◽

Vol 42 (2) ◽

pp. 65-68 ◽

Cited By ~ 1

Author(s):

Liv Skartveit ◽

Anne Bjørrg Tveit ◽

Björn Klinge ◽

Bård Tørtdal ◽

Knut Selvig

Keyword(s):

In Vivo Uptake

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Antibody against low density lipoprotein receptor blocks uptake of low density lipoprotein (but not high density lipoprotein) by the adrenal gland of the mouse in vivo.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)69305-1 ◽

1981 ◽

Vol 256 (10) ◽

pp. 4701-4703

Author(s):

T. Kita ◽

U. Beisiegel ◽

J.L. Goldstein ◽

W.J. Schneider ◽

M.S. Brown

Keyword(s):

Adrenal Gland ◽

High Density Lipoprotein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Density ◽

Low Density ◽

Lipoprotein Receptor ◽

Low Density Lipoprotein Receptor ◽

Density Lipoprotein Receptor

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Secretion of Pregn-4-ene-3,20-dione (Progesterone) in vivo by the Adrenal Gland of the Rat

Nature ◽

10.1038/221572b0 ◽

1969 ◽

Vol 221 (5180) ◽

pp. 572-573 ◽

Cited By ~ 24

Author(s):

MARGARETHE HOLZBAUER ◽

HELEN M. NEWPORT ◽

MARION K. BIRMINGHAM ◽

HELLI TRAIKOV

Keyword(s):

Adrenal Gland

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Maturation, iron deficiency, and ligands in enteric radioiron transport in vitro

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1963.204.1.171 ◽

1963 ◽

Vol 204 (1) ◽

pp. 171-175 ◽

Cited By ~ 14

Author(s):

W. S. Ruliffson ◽

J. M. Hopping

Keyword(s):

Ascorbic Acid ◽

Iron Deficiency ◽

Iron Absorption ◽

Deficiency Anemia ◽

Anaerobic Incubation ◽

Reverse Effect ◽

Factors Affecting ◽

Everted Gut Sac

The effects in rats, of age, iron-deficiency anemia, and ascorbic acid, citrate, fluoride, and ethylenediaminetetraacetate (EDTA) on enteric radioiron transport were studied in vitro by an everted gut-sac technique. Sacs from young animals transported more than those from older ones. Proximal jejunal sacs from anemic animals transported more than similar sacs from nonanemic rats, but the reverse effect appeared in sacs formed from proximal duodenum. When added to media containing ascorbic acid or citrate, fluoride depressed transport as did anaerobic incubation in the presence of ascorbic acid. Anaerobic incubation in the presence of EDTA appeared to permit elevated transport. Ascorbic acid, citrate, and EDTA all enhanced the level of Fe59 appearing in serosal media. These results appear to agree with previously established in vivo phenomena and tend to validate the in vitro method as one of promise for further studies of factors affecting iron absorption and of the mechanism of iron absorption.

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