Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus

FEBS Letters ◽  
1998 ◽  
Vol 436 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Silvia Russo ◽  
Laura Montermini ◽  
Revital Berkovitz-Siman-Tov ◽  
Wilma Ponti ◽  
Giorgio Poli
Keyword(s):  
Bovine Leukemia Virus ◽  
Insect Cells ◽  
Leukemia Virus ◽  
Recombinant Baculovirus ◽  
Env Glycoprotein

Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease

2012 ◽  
Vol 54 (2) ◽  
pp. 475-483 ◽  
Author(s):  
Alejandra Larsen ◽  
Ester Teresa Gonzalez ◽  
María Soledad Serena ◽  
María Gabriela Echeverría ◽  
Eduardo Mortola
Keyword(s):  
Bovine Leukemia Virus ◽  
Insect Cells ◽  
Leukemia Virus ◽  
Gag Protein

scholarly journals Bovine Leukemia Virus Gag Particle Assembly in Insect Cells: Formation of Chimeric Particles by Domain-Switched Leukemia/Lentivirus Gag Polyprotein

Virology ◽  
1999 ◽  
Vol 265 (2) ◽  
pp. 308-318 ◽  
Author(s):  
Naresh K. Kakker ◽  
Michael V. Mikhailov ◽  
Milan V. Nermut ◽  
Arsene Burny ◽  
Polly Roy
Keyword(s):  
Bovine Leukemia Virus ◽  
Insect Cells ◽  
Leukemia Virus ◽  
Particle Assembly ◽  
Gag Polyprotein

scholarly journals Expression of the Bovine Leukemia Virus Envelope Glycoprotein (gp51) by Recombinant Baculovirus and Its Use in an Enzyme-Linked Immunosorbent Assay

2004 ◽  
Vol 11 (1) ◽  
pp. 147-151 ◽  
Author(s):  
Antonio De Giuseppe ◽  
Francesco Feliziani ◽  
Domenico Rutili ◽  
Gian Mario De Mia
Keyword(s):  
Immunosorbent Assay ◽  
Envelope Glycoprotein ◽  
Bovine Leukemia Virus ◽  
Signal Sequence ◽  
Leukemia Virus ◽  
Recombinant Baculovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Envelope ◽  
Gene Encoding ◽  
Field Samples

ABSTRACT The gene encoding the major envelope glycoprotein (gp51) with its signal sequence, represented by an additional NH2-terminal 33-residue amino acid sequence of bovine leukemia virus (BLV), was inserted into a baculovirus transfer vector. A recombinant virus expressing a secreted gp51 protein in insect cells was isolated. The recombinant gp51 expressed was characterized by using an anti-BLV monoclonal antibody by both Western blotting analysis and enzyme-linked immunosorbent assay (ELISA). The secreted gp51 was used as an antigen, and an ELISA with recombinant gp51 (rgp51) was developed for the detection of BLV antibodies. This new procedure was compared with a previous ELISA method for the detection of BLV antibodies and an agar gel immunodiffusion test performed with an unpurified BLV antigen preparation. The comparative testing of field samples showed that the ELISA with rgp51 is more specific and also suitable for the testing of pooled sera.

A STUDY OF HETEROGENEITY OF BOVINE LEUKEMIA VIRUS GENOTYPES IN CATTLE

2012 ◽  
pp. 69-72
Author(s):  
N.V. Bateneva ◽  
◽  
P.N. Smirnov ◽  
I.V. Mikhnovich ◽  
◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Virus Genotypes

Bovine Leukemia Virus in Bovine Aborted Fetuses

2019 ◽  
Vol 07 (01) ◽  
Author(s):  
Kelly Cristina Santos Montanari ◽  
Marcia Mayumi Fusuma ◽  
Alessandra Maria Dias Lacerda ◽  
Líria Hiromi Okuda ◽  
Edviges Maristela Pituco ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus

A Serological Study on Bovine Leukemia Virus Infection in Ten Provinces of Iran between 2010 and 2012

2012 ◽  
Vol 6 (3) ◽  
pp. 1-7 ◽  
Author(s):  
M Kazemimanesh ◽  
O Madadgar ◽  
MR Mahzoonieh ◽  
T Zahraei-Salehi ◽  
F Steinbach ◽  
...  
Keyword(s):  
Virus Infection ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Leukemia Virus Infection ◽  
Serological Study
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