GENOTYPIC IDENTITY OF BOVINE LEUKEMIA VIRUS ISOLATES, CIRCULATING IN LIVESTOCK POPULATIONS OF THE REPUBLIC OF TATARSTAN

Author(s):  
Kh.Kh. Gilmanov ◽  
◽  
R.R. Vafin ◽  
A.Y. Shaeva ◽  
Z.R. Zakirova ◽  
...  
2015 ◽  
Vol 12 (1) ◽  
Author(s):  
EunJung Lee ◽  
Eun-Ju Kim ◽  
Ha-Kyung Joung ◽  
Bo-Hye Kim ◽  
Jae-Young Song ◽  
...  

2019 ◽  
Vol 164 (6) ◽  
pp. 1697-1703 ◽  
Author(s):  
Moira Bazzucchi ◽  
Carmen Iscaro ◽  
Cristina Casciari ◽  
Monica Giammarioli ◽  
Francesco Feliziani

2021 ◽  
Vol 0 (14) ◽  
pp. 70-80
Author(s):  
Maksim Petropavlovskiy ◽  
A. Lysov ◽  
Al'bina Isaeva ◽  
Alisa Romanova

Abstract. The study of the features of the leukemic epizootic process in agricultural organizations of the Republic of Bashkortostan was carried out. The object of the study was cattle of different groups, including calves before drinking colostrum, heifers, springer heifers, pregnant and dairy cows. The purpose was to develop general schemes of anti-leukemic measures that guarantee a reduction in the recovery period for disadvantaged areas and a decrease in the level of infection of cattle with the leukemia virus. The work was carried out in the leukemia laboratory of the department for monitoring and predicting infectious animal diseases of the Ural Federal Agrarian Scientific Research Center of the Ural Branch of the Russian Academy of Sciences. Methods. A retrospective and operational analysis of data on the laboratory methods used for the diagnosis of bovine leukemia virus was carried out. Scientific novelty consists in obtaining new knowledge about the problems of diagnostics of the bovine leukemia virus among the populations of farm animals in the Republic of Bashkortostan. The features of the leukemic epizootic process have been studied: sources, transmission routes of the leukemia virus, level of infection and incidence of bovine leukemia virus in the surveyed farms in the regions of the Republic of Bashkortostan. Results. On the basis of the data obtained, general schemes of anti-leukemic measures have been developed, which guarantee a reduction in the recovery time of disadvantaged areas and a decrease in the level of infection of livestock with the leukemia virus.


2020 ◽  
Vol 3 (43) ◽  
pp. 144-149
Author(s):  
MUSTAFAEV A. R. ◽  
◽  
DZHAMBULATOV Z. M. ◽  
GADZHIEV B. M. ◽  
◽  
...  

2020 ◽  
pp. 5-7
Author(s):  
Roman A. Krivonos ◽  
◽  
Aleksey A. Rusinovich ◽  
Nikolay S. Motuzko ◽  
Aleksandr A. Lysenko ◽  
...  

One of the problems in dairy cattle breeding has become bovine leukemia since the middle of the 20th century in the Republic of Belarus. Initially, isolated cases of the disease were recorded according to the data of clinical signs and pathological changes in the case of death of animals and the results of veterinary sanitary examination at the section of meat processing plants. After the establishment of the infectious etiology of the disease and the introduction of the immune diffusion reaction into veterinary laboratory practice, the massive nature of its spread was established with corresponding economic losses for the livestock industry. Under the prevailing conditions, it was necessary to reliably study the indicators of the epizootic situation and the trends of the epizootic process affecting the spread of infection with the bovine leukemia virus in order to take effective measures to eliminate it. The authors considered the methodological approaches to the study of the epizootic situation and the dynamics of the epizootic process of bovine leukemia virus infection in the Republic of Belarus formed the basis of the developed monitoring system. It included 132 epizootic significant indicators, divided into 8 groups, reflecting the epizootic situation of bovine leukemia virus infection, taking into account age, sex, production, territorial and other characteristics of infectious and epizootic processes of the disease in the conditions of the Republic of Belarus, established the procedure for collecting and transmitting epizootic information for making management decisions. This made it possible to control the epizootic situation and predict its development, to determine the features and patterns of the epizootic process, to evaluate the results of diagnostic studies, the effectiveness of anti-leukemic measures and their socio-economic significance. The use of epizootic, clinical, pathomorphological, laboratory methods of research with subsequent statistical processing of the data obtained and their analysis in the monitoring system of infection of the cattle leukemia virus in the Republic of Belarus made it possible to control the epizootic situation, predict its development, determine the features and patterns of the epizootic process, evaluate the results of diagnostic research, the effectiveness of anti-leukemic measures and their socio-economic significance.


2019 ◽  
Vol 191 (12) ◽  
pp. 52-59
Author(s):  
Natalia Bezborodova ◽  
M. Petropavlovskiy ◽  
A. Romanova ◽  
A. Lysov ◽  
Veronika Kozhukhovskaya

Abstract. Scientific novelty. The article presents the materials of long-term work on the use of PCR diagnostics together with serological methods (rid, ELISA) in the study of bovine leukemia virus of various physiological groups, as well as experimental animals (rabbits, small cattle).The aim of the study was to evaluate the PCR method in the diagnosis of bovine leukemia virus. Methods. Biomaterials (blood, colostrum) from cattle for PCR studies were obtained from 20 agricultural organizations of Tyumen, Chelyabinsk, Kurgan regions and the Republic of Bashkortostan. A total of 1,269 blood samples and 36 colostrum samples were examined by PCR. Laboratory methods (rid, ELISA, PCR) were used in the early diagnosis of leukemia virus in young cattle, as well as in the diagnosis of adult livestock. Results. The results obtained by PCR revealed carriers of the Lekota virus in calves aged 15 days to 1 month among young animals, which significantly reduced the duration of recreational activities (an average of 20 %). It was established that serological examination of newborn calves for the presence of antibodies to leukemia virus can give false results due to the influence of high concentrations of maternal antibodies and the development of immunological tolerance. PCR studies of biomaterial (samples 1023), taken from adult cattle (cows 3–4, 6 years), showed that 52 % of the samples were present leukemia virus, which was confirmed by serological studies. It is proved that up to 20 % of virus carriers from the number of rid-negative animals are detected during ELISA and PCR. Some types of BLV leukemia can not be detected by serological studies, but easily cope with PCR. The article deals with various types of PCR used in the diagnosis of leukemia, describes the data obtained from experiments on artificially infected animals with leukemia virus.


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