Cryogenic scanning transmission microscopy for biochemical analysis of the sperm.

2001 ◽  
Vol 76 (3) ◽  
pp. S265
Author(s):  
A Osanna ◽  
C.J Jacobsen ◽  
J Kirz ◽  
Y.R Sheynkin
Keyword(s):  
Biochemical Analysis ◽  
Transmission Microscopy ◽  
Scanning Transmission

Scanning Transmission Microscopy Applied to Thick Specimen

1972 ◽  
Vol 30 ◽  
pp. 452-453
Author(s):  
H. Koike ◽  
T. Matsuo ◽  
K. Ueno ◽  
M. Suzuki
Keyword(s):  
Psoas Muscle ◽  
Image Intensifier ◽  
Chromatic Aberration ◽  
Image Contrast ◽  
Transmission Microscopy ◽  
Crystalline Materials ◽  
Thick Specimen ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Electron Microscope Image

Since the identification of single atoms was achieved by Crewe et al, scanning transmission microscopy has been put into pratical use. Recently they applied this method to the quantitative mass analysis of DNA.As pointed out previously the chromatic aberration which decreases the image contrast and quality, does not affect a scanning transmission image as it does a conventional transmission electron microscope image. Thus, the STEM method is advantageous for thick specimen. Further this method employs a high sensitive photomultiplier tube which also functions as an image intensifier. This detection method is effective for the observation of living specimens or easily damaged specimens. In this respect the scanning transmission microscope with high accelerating voltage is necessary.Since Uyeda's experiments of crystalline materials, many workers have been discussed how thick specimens can be observed by CTEM. With biological specimens, R. Szirmae reported on the decrease in the image contrast of rabbit psoas muscle sections at various accelerating voltages and specimen thicknesses.

Transmission Scanning Electron Microscopy and Energy Analysis with the Siemens ELMISKOP 101

1972 ◽  
Vol 30 ◽  
pp. 450-451
Author(s):  
H. M. Thieringer
Keyword(s):  
Objective Lens ◽  
Transmission Microscopy ◽  
Image Recording ◽  
Mode Of Operation ◽  
Scanning Transmission ◽  
Electron Microscopes ◽  
And Performance ◽  
Convergent Beam ◽  
Ray Path ◽  
Beam Diffraction

It has repeatedly been show that with conventional electron microscopes very fine electron probes can be produced, therefore allowing various micro-techniques such as micro recording, X-ray microanalysis and convergent beam diffraction. In this paper the function and performance of an SIEMENS ELMISKOP 101 used as a scanning transmission microscope (STEM) is described. This mode of operation has some advantages over the conventional transmission microscopy (CTEM) especially for the observation of thick specimen, in spite of somewhat longer image recording times.Fig.1 shows schematically the ray path and the additional electronics of an ELMISKOP 101 working as a STEM. With a point-cathode, and using condensor I and the objective lens as a demagnifying system, an electron probe with a half-width ob about 25 Å and a typical current of 5.10-11 amp at 100 kV can be obtained in the back focal plane of the objective lens.

Microstructural examination of modified yttria stabilized zirconia by EM analytical techniques

1986 ◽  
Vol 44 ◽  
pp. 842-843
Author(s):  
W. W. Davison ◽  
R. C. Buchanan
Keyword(s):  
Mechanical Properties ◽  
Analytical Techniques ◽  
Yttria Stabilized Zirconia ◽  
Stabilized Zirconia ◽  
Transmission Microscopy ◽  
Sintering Aid ◽  
Densification Temperature ◽  
Chemical Inertness ◽  
Scanning Transmission ◽  
Microstructural Examination

Yttria stabilized zirconia (YSZ) has become a significant technological material due to its high ionic conductivity, chemical inertness, and good mechanical properties. Temperatures on the order of 1700°C are required, however, to densify YSZ to the degree necessary for good electrical and mechanical properties. A technique for lowering the densification temperature is the addition of small amounts of material which facilitate the formation of a liquid phase at comparatively low temperatures. In this study, sintered microstructures obtained from the use of Al2O3 as a sintering aid were examined with scanning, transmission, and scanning transmission microscopy (SEM, TEM, and STEM).

Using saturated absorption for super‐resolution laser scanning transmission microscopy

2021 ◽  
Author(s):  
Kentaro Nishida ◽  
Hikaru Sato ◽  
Ryosuke Oketani ◽  
Kentaro Mochizuki ◽  
Kenta Temma ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Super Resolution ◽  
Transmission Microscopy ◽  
Saturated Absorption ◽  
Scanning Transmission

Scanning, Transmission and Electron Probe Analysis of the Cat Tapetum

1975 ◽  
Vol 33 ◽  
pp. 702-703
Author(s):  
C. E. Turnbill
Keyword(s):  
Close Correlation ◽  
High Energy ◽  
Uranyl Acetate ◽  
High Energy Particle ◽  
Electron Probe Analysis ◽  
Transmission Microscopy ◽  
Retinal Rods ◽  
Cacodylate Buffer ◽  
Scanning Transmission ◽  
Adult Cats

The tapetum lucidum or “bright carpet” of the cat eye is an adaptation for vision in limited light. The tapetum is of particular interest to our laboratory in that it might be used as a visual maker for cosmic “heavy primary” radiation. Since this marker would be located rear the retina, it would provide close correlation between high energy particle trajectories and related retinal damage. In preparation for this task, the cellular tapetune of adult cats were examined as controls.Adult cats were perfused with 2% glutaralderhyde, 2% paraformaldehyde and placed in cacodylate buffer. The tapetune was separated from the retina and choroid and processed for either scanning or transmission microscopy.Material for the transmission microscope was postfixed in 1% osmium tetroxide and examined both stained, with uranyl acetate and lead, and unstained for microprobe analysis. The stained sections (Fig. 1) cut parallel to the axis of the retinal rods, show layers of cells filled with electron dense rod-like structures approximately 10 microns by .1 micron.

scholarly journals Confocal soft X-ray scanning transmission microscopy: setup, alignment procedure and limitations

2015 ◽  
Vol 22 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Andreas Späth ◽  
Jörg Raabe ◽  
Rainer H. Fink
Keyword(s):  
Magnetic Thin Films ◽  
Biological Tissues ◽  
Polymer Materials ◽  
Zone Plate ◽  
Contrast Imaging ◽  
Transmission Microscopy ◽  
X Ray ◽  
Alignment Procedure ◽  
High Contrast Imaging ◽  
Scanning Transmission

Zone-plate-based scanning transmission soft X-ray microspectroscopy (STXM) is a well established technique for high-contrast imaging of sufficiently transparent specimens (e.g.ultrathin biological tissues, polymer materials, archaeometric specimens or magnetic thin films) with spatial resolutions in the regime of 20 nm and high spectroscopic or chemical sensitivity. However, due to the relatively large depth of focus of zone plates, the resolution of STXM along the optical axis so far stays unambiguously behind for thicker X-ray transparent specimens. This challenge can be addressed by the implementation of a second zone plate in the detection pathway of the beam, resulting in a confocal arrangement. Within this paper a first proof-of-principle study for a confocal STXM (cSTXM) and an elaborate alignment procedure in transmission and fluorescence geometry are presented. Based on first confocal soft X-ray micrographs of well known specimens, the advantage and limitation of cSTXM as well as further development potentials for future applications are discussed.

scholarly journals Soft X-ray Scanning Transmission Microscopy Studies of Radiation Damage by Electron, Ion and X-ray Beams

2020 ◽  
Vol 26 (S2) ◽  
pp. 2072-2074
Author(s):  
Adam Hitchcock ◽  
Hao Yuan ◽  
Lis Melo ◽  
Nabil Basim
Keyword(s):  
Radiation Damage ◽  
Transmission Microscopy ◽  
X Ray ◽  
Scanning Transmission
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