Using saturated absorption for super‐resolution laser scanning transmission microscopy

2021 ◽  
Author(s):  
Kentaro Nishida ◽  
Hikaru Sato ◽  
Ryosuke Oketani ◽  
Kentaro Mochizuki ◽  
Kenta Temma ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Super Resolution ◽  
Transmission Microscopy ◽  
Saturated Absorption ◽  
Scanning Transmission

Scanning Transmission Microscopy Applied to Thick Specimen

1972 ◽  
Vol 30 ◽  
pp. 452-453
Author(s):  
H. Koike ◽  
T. Matsuo ◽  
K. Ueno ◽  
M. Suzuki
Keyword(s):  
Psoas Muscle ◽  
Image Intensifier ◽  
Chromatic Aberration ◽  
Image Contrast ◽  
Transmission Microscopy ◽  
Crystalline Materials ◽  
Thick Specimen ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Electron Microscope Image

Since the identification of single atoms was achieved by Crewe et al, scanning transmission microscopy has been put into pratical use. Recently they applied this method to the quantitative mass analysis of DNA.As pointed out previously the chromatic aberration which decreases the image contrast and quality, does not affect a scanning transmission image as it does a conventional transmission electron microscope image. Thus, the STEM method is advantageous for thick specimen. Further this method employs a high sensitive photomultiplier tube which also functions as an image intensifier. This detection method is effective for the observation of living specimens or easily damaged specimens. In this respect the scanning transmission microscope with high accelerating voltage is necessary.Since Uyeda's experiments of crystalline materials, many workers have been discussed how thick specimens can be observed by CTEM. With biological specimens, R. Szirmae reported on the decrease in the image contrast of rabbit psoas muscle sections at various accelerating voltages and specimen thicknesses.

Transmission Scanning Electron Microscopy and Energy Analysis with the Siemens ELMISKOP 101

1972 ◽  
Vol 30 ◽  
pp. 450-451
Author(s):  
H. M. Thieringer
Keyword(s):  
Objective Lens ◽  
Transmission Microscopy ◽  
Image Recording ◽  
Mode Of Operation ◽  
Scanning Transmission ◽  
Electron Microscopes ◽  
And Performance ◽  
Convergent Beam ◽  
Ray Path ◽  
Beam Diffraction

It has repeatedly been show that with conventional electron microscopes very fine electron probes can be produced, therefore allowing various micro-techniques such as micro recording, X-ray microanalysis and convergent beam diffraction. In this paper the function and performance of an SIEMENS ELMISKOP 101 used as a scanning transmission microscope (STEM) is described. This mode of operation has some advantages over the conventional transmission microscopy (CTEM) especially for the observation of thick specimen, in spite of somewhat longer image recording times.Fig.1 shows schematically the ray path and the additional electronics of an ELMISKOP 101 working as a STEM. With a point-cathode, and using condensor I and the objective lens as a demagnifying system, an electron probe with a half-width ob about 25 Å and a typical current of 5.10-11 amp at 100 kV can be obtained in the back focal plane of the objective lens.

Microstructural examination of modified yttria stabilized zirconia by EM analytical techniques

1986 ◽  
Vol 44 ◽  
pp. 842-843
Author(s):  
W. W. Davison ◽  
R. C. Buchanan
Keyword(s):  
Mechanical Properties ◽  
Analytical Techniques ◽  
Yttria Stabilized Zirconia ◽  
Stabilized Zirconia ◽  
Transmission Microscopy ◽  
Sintering Aid ◽  
Densification Temperature ◽  
Chemical Inertness ◽  
Scanning Transmission ◽  
Microstructural Examination

Yttria stabilized zirconia (YSZ) has become a significant technological material due to its high ionic conductivity, chemical inertness, and good mechanical properties. Temperatures on the order of 1700°C are required, however, to densify YSZ to the degree necessary for good electrical and mechanical properties. A technique for lowering the densification temperature is the addition of small amounts of material which facilitate the formation of a liquid phase at comparatively low temperatures. In this study, sintered microstructures obtained from the use of Al2O3 as a sintering aid were examined with scanning, transmission, and scanning transmission microscopy (SEM, TEM, and STEM).

scholarly journals Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 861
Author(s):  
Jacopo Cardellini ◽  
Arianna Balestri ◽  
Costanza Montis ◽  
Debora Berti
Keyword(s):  
Drug Delivery ◽  
Fluorescence Microscopy ◽  
Drug Delivery Systems ◽  
Laser Scanning ◽  
Super Resolution ◽  
Laser Scanning Confocal Microscopy ◽  
Delivery Systems ◽  
Scanning Confocal Microscopy ◽  
Biological Phenomena

In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues or entire organisms or the extent of their cellular uptake (in vitro). However, the diffraction limit of light, which limits the resolution to hundreds of nanometers, has for long time restricted the extent and quality of information and insight achievable through these techniques. The advent of super-resolution microscopic techniques, recognized with the 2014 Nobel prize in Chemistry, revolutionized the field thanks to the possibility to achieve nanometric resolution, i.e., the typical scale length of chemical and biological phenomena. Since then, fluorescence microscopy-related techniques have acquired renewed interest for the scientific community, both from the perspective of instrument/techniques development and from the perspective of the advanced scientific applications. In this contribution we will review the application of these techniques to the field of drug delivery, discussing how the latest advancements of static and dynamic methodologies have tremendously expanded the experimental opportunities for the characterization of drug delivery systems and for the understanding of their behaviour in biologically relevant environments.

scholarly journals Structure of the germarium and female germ-cell clusters in Thulinius ruffoi (Bertolani, 1982) (Tardigrada: Eutardigrada: Parachela)

2019 ◽  
Author(s):  
Kamil Janelt ◽  
Marta Jezierska ◽  
Sebastian Student ◽  
Izabela Poprawa
Keyword(s):  
Germ Cell ◽  
Laser Scanning ◽  
Spatial Organization ◽  
Germ Line ◽  
Anterior Part ◽  
Confocal Laser ◽  
Freshwater Species ◽  
Cell Clusters ◽  
Scanning Transmission ◽  
Female Germ Cell

Abstract Thulinius ruffoi is a freshwater species that has the ability to reproduce via parthenogenesis. A meroistic polytrophic ovary is present in this species. Analyses of the germarium structure, and formation and organization of female germ-cell clusters were performed using light, confocal laser scanning, transmission electron and serial block-face scanning electron microscopy. The germarium is the small, anterior part of an ovary that contains putative germ-line stem cells. In the studied species, the female germ-cell clusters are large and branched. Only one cell in each cluster develops into an oocyte, while all the other cells become trophocytes. In this paper, we present the first report on the presence of F-actin as a component of the intercellular bridges that connect the cells in the germ-cell cluster of T. ruffoi. Moreover, our results show that the female germ-cell clusters are formed as the result of both synchronous and asynchronous divisions and that their organization can vary not only between individuals of the investigated species, but also that clusters developing simultaneously within the same ovary can have a different spatial organization.

scholarly journals Full-field structured-illumination super-resolution X-ray transmission microscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Benedikt Günther ◽  
Lorenz Hehn ◽  
Christoph Jud ◽  
Alexander Hipp ◽  
Martin Dierolf ◽  
...  
Keyword(s):  
Super Resolution ◽  
Structured Illumination ◽  
Transmission Microscopy ◽  
Full Field ◽  
X Ray

Cryogenic scanning transmission microscopy for biochemical analysis of the sperm.

2001 ◽  
Vol 76 (3) ◽  
pp. S265
Author(s):  
A Osanna ◽  
C.J Jacobsen ◽  
J Kirz ◽  
Y.R Sheynkin
Keyword(s):  
Biochemical Analysis ◽  
Transmission Microscopy ◽  
Scanning Transmission

Super-resolution Microscope Based on Laser Scanning and a Microsphere Lens

2014 ◽  
Author(s):  
Kuan-Yu Li ◽  
Yun-Ju Liu ◽  
Yang Tsao ◽  
Kung-Hsuan Lin ◽  
Chih-Wei Chang ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Super Resolution
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