Effect of Chilling on Nitrate Reductase in the Leaves and Roots of Cucumber (Cucumis sativus L.)

Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule are used as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected. Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callus induction response is compared with cultures under 4-week cultured on CBM medium supplemented with various concentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 – 0.20 mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries were transferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBM with TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovaries become green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested from ovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.

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Reduction of nitrates in Cucumis sativus L. seedlings I. Influence of tungsten and vanadium on absorption and reduction of nitrates

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1980.023 ◽

2014 ◽

Vol 49 (3) ◽

pp. 259-267 ◽

Cited By ~ 1

Author(s):

Józef Buczek ◽

Ewa Kowalińska ◽

Krystyna Kuczera

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Plant Tissues ◽

Inhibiting Activity ◽

Cucumis Sativus L ◽

Cucumber Seedlings

Investigations were conducted on the influence of tungsten (Na2WO4) and meta-vanadate (NaVO3) on uptake and reduction of nitrates by cucumber seedlings. Tungsten and vanadium almost completely inhibited nitrate reductase activity (NR) after treating the plants for 24 hours with nitrates in the presence of 10-4 M WO4-2 and 10-3 M VO3-. Uptake of NO3- in the presence of WO4-2 in this same period of time was lowered by about 50 %, while in the presence of VO3- by seventy percent. The ratio of NO3- absorbed to that reducted in control plants was around 3, whereas in the presence of WO4-2 and VO3- 9 and 8 respectively. The effect of NR inhibiting activity by WO4-2 and VO3- was significant but somewhat weaker if both inhibitors were applied to the plants 24 hours after formation of the NR system, whereas NO3- uptake was subject to a slight lowering. The above data suggest that WO4-2 and VO3- after a 24 hour application of these inhibitors to plants, primary inhibit biosynthesis and activity of NR and to a lesser extent effect NO3- absorption. On the basis of results reached the Butz and Jackson (1977) hypothesis concerning the model of NO3- uptake and reduction by plant tissues is discussed.

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Protein oxidation in the leaves and roots of cucumber plants (Cucumis sativus L.), mutant MSC16 and wild type

Journal of Plant Physiology ◽

10.1016/j.jplph.2007.06.021 ◽

2008 ◽

Vol 165 (4) ◽

pp. 355-365 ◽

Cited By ~ 19

Author(s):

Izabela M. Juszczuk ◽

Agnieszka Tybura ◽

Anna M. Rychter

Keyword(s):

Cucumis Sativus ◽

Protein Oxidation ◽

Wild Type ◽

Cucumis Sativus L ◽

Leaves And Roots

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Influence of simazine on nitrate uptake and nitrate reductase activity in Triticum aestivum (L.) and Cucumis sativus (L.) seedlings

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1984.023 ◽

2014 ◽

Vol 53 (2) ◽

pp. 247-256 ◽

Cited By ~ 1

Author(s):

Irena Jarzyńska ◽

Józef Buczek

Keyword(s):

Triticum Aestivum ◽

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Uptake ◽

Reductase Activity ◽

Triticum Aestivum L ◽

Probable Mechanism ◽

Cucumis Sativus L ◽

Photosynthesis Inhibition ◽

Nr Activity

The influence of simazine (2-chloro-4,6-bis(ethylamine)-1,3,5-triazine) on N03- uptake and nitrate reductase (NR) activity in wheat (Triticum aestivum) and cucumber (Cucumis sativus) seedlings was investigated. It was found that at first (up to 12 h after herbicide treatment) simazine enhanced and then inhibited NR activity in the leaves and roots of the tested plants. After seven days of growth in nutrient medium containing 2.5 and 25 µM simazine the herbicide inhibited by 25 and 70 per cent, respectively, NR activity and reduced by 30 per cent in wheat and 50 per cent in cucumber seedlings nitrate uptake. The probable mechanism of NR inhibition by simazin is discussed and it is suggested that the influence of the herbicide on NR activity may not be exclusively connected with photosynthesis inhibition.

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