Protein oxidation in the leaves and roots of cucumber plants (Cucumis sativus L.), mutant MSC16 and wild type

Haploid plants achieve through androgenesis or gynogenesis. In gynogenesis method, the ovary or ovule are used as explants induct haploid plants. Female flower one day before flowering of Cucumis sativus L. are collected. Cold pretreatment of ovaries at 4°C up to 24 hours and culture under dark conditions. Significantly enhanced callus induction response is compared with cultures under 4-week cultured on CBM medium supplemented with various concentration of TDZ 0.01-0.04 mg/L. After 4 weeks, ovaries are transferred to medium with kinetin 0.05 – 0.20 mg/L. Then, ovaries were transferred to medium supplemented with BA: IAA 3:1. Finally, green ovaries were transferred to BA 1.5 mg/L and GA3 1.5 mg/L. The results showed that ovary induction has best affected on CBM with TDZ 0.03 mg/L with 11 callus/sample. Ovaries developed on kinetin 0.1 mg/L with 7.4 callus/sample. Ovaries become green and had leaves and roots formation on BA: IAA (3 mg/L: 1 mg/L). 11 plantlets were harvested from ovary culture after 12-week culture on CBM supplemented with BA 1.5 mg/L and GA3 1.5 mg/L.

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Effect of Chilling on Nitrate Reductase in the Leaves and Roots of Cucumber (Cucumis sativus L.)

Biochemie und Physiologie der Pflanzen ◽

10.1016/s0015-3796(82)80049-8 ◽

1982 ◽

Vol 177 (7) ◽

pp. 557-566 ◽

Cited By ~ 1

Author(s):

Marie Bláhová

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Cucumis Sativus L ◽

Leaves And Roots

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Factors Enhancing Virulence Response of Agrobacteria in Cucumber (Cucumis sativus L.)

The Agriculturists ◽

10.3329/agric.v7i1.5256 ◽

2010 ◽

pp. 72-81

Author(s):

AKM Mohiuddin ◽

Zaliha C Abdullah ◽

MKU Chowdhury ◽

K Harikrishna ◽

Suhaimi Napis

Keyword(s):

Light Intensity ◽

Cucumis Sativus ◽

Hairy Roots ◽

Tissue Type ◽

Physical Factors ◽

Wild Type ◽

Agrobacterium Strain ◽

Cucumis Sativus L ◽

Chemical Factors ◽

Physical And Chemical

The virulence response of five wild type Agrobacterium tumefaciens (tumorigenic) strains and five wild type A. rhizogenes (oncogenic) strains was studied in cucumber (Cucumis sativus L.), cv. Spring Swallow. Several physical and chemical factors e.g. Agrobacterium strains, tissue type (stem and petiole tissues), light intensity, dark incubation, concentrations of acetosyringone (a phenolic compound) were tested. The virulent response of Agrobacteria was found to be depend on plant tissue type, Agrobacterium strain, tissue-Agrobacterium interaction and both chemical-physical factors. Specific light intensity, 2500-lux, as well as the acetosyringone concentration, 20-mM, played important role in virulence response. Among the different strains, LBA 4404 and 15834 were highly virulent to cucumber compared to the other strains tested. A. tumefaciens strains A348 and A281 were failed to initiate crown galls in both stem and petiole tissues, however, in the presence of acetosyringone they produced crown galls. Similarly, A. rhizogenes strain A4 initiated hairy roots in the presence of acetosyringone. All developed crown galls and hairy roots showed an autonomous growth on a hormone free medium containing cefatoxime. Thus, enhancement of virulence response was not only dependent on Agrobacterium strains, but also on particular physical and chemical factors also play vital role.

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