Reduction of nitrates in Cucumis sativus L. seedlings I. Influence of tungsten and vanadium on absorption and reduction of nitrates

Investigations were conducted on the influence of tungsten (Na2WO4) and meta-vanadate (NaVO3) on uptake and reduction of nitrates by cucumber seedlings. Tungsten and vanadium almost completely inhibited nitrate reductase activity (NR) after treating the plants for 24 hours with nitrates in the presence of 10-4 M WO4-2 and 10-3 M VO3-. Uptake of NO3- in the presence of WO4-2 in this same period of time was lowered by about 50 %, while in the presence of VO3- by seventy percent. The ratio of NO3- absorbed to that reducted in control plants was around 3, whereas in the presence of WO4-2 and VO3- 9 and 8 respectively. The effect of NR inhibiting activity by WO4-2 and VO3- was significant but somewhat weaker if both inhibitors were applied to the plants 24 hours after formation of the NR system, whereas NO3- uptake was subject to a slight lowering. The above data suggest that WO4-2 and VO3- after a 24 hour application of these inhibitors to plants, primary inhibit biosynthesis and activity of NR and to a lesser extent effect NO3- absorption. On the basis of results reached the Butz and Jackson (1977) hypothesis concerning the model of NO3- uptake and reduction by plant tissues is discussed.

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Influence of pH on Cd and Cu uptake, distribution and their effect on nitrate reductase activity in cucumber (Cucumis sativus L.) seedling roots

Acta Physiologiae Plantarum ◽

10.1007/s11738-001-0009-x ◽

2001 ◽

Vol 23 (2) ◽

pp. 201-206 ◽

Cited By ~ 7

Author(s):

Marek Burzyński

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Cucumis Sativus L ◽

Seedling Roots ◽

Influence Of Ph ◽

Cu Uptake

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Effect of different oxygen availability on the nitrate reductase activity in Cucumis sativus roots

Biologia Plantarum ◽

10.1007/s10535-008-0130-4 ◽

2008 ◽

Vol 52 (4) ◽

pp. 674-680 ◽

Cited By ~ 3

Author(s):

M. Reda ◽

G. Klobus

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Oxygen Availability

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Light modulation and in vitro effects of adenine nucleotides on leaf nitrate reductase activity in cucumber (Cucumis sativus)

Physiologia Plantarum ◽

10.1034/j.1399-3054.1999.105205.x ◽

1999 ◽

Vol 105 (2) ◽

pp. 218-223 ◽

Cited By ~ 10

Author(s):

Eloísa Agüera ◽

Lourdes Poblete ◽

Purificación de la Haba ◽

José M. Maldonado

Keyword(s):

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Adenine Nucleotides ◽

Light Modulation ◽

In Vitro Effects ◽

Leaf Nitrate

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Relationships between nitrate uptake and nitrate reductase activity in Cucumis sativus L.

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1998.032 ◽

2014 ◽

Vol 67 (3-4) ◽

pp. 253-257

Author(s):

Grażyna Kłobus ◽

Jolanta Jerzykiewicz ◽

Józef Buczek

Keyword(s):

Plasma Membrane ◽

Nitrate Reductase ◽

Nitrate Reductase Activity ◽

Nitrate Uptake ◽

Plasma Membranes ◽

Polyclonal Antibodies ◽

Reductase Activity ◽

Nitrate Transport ◽

Plasma Membrane Vesicles ◽

Cucumis Sativus L

Anti-NR IgG fragments obtained after papain digestion of polyclonal antibodies gave the positive immunological reaction with both, a soluble and plasma membrane-bound nitrate reductase. Anti-NR antibody as well as IgG fragments almost totally inhibited the nitrate reductase activity in cytosol proving a crossreaction of antibody with the catalytic site of a soluble NR. Anti-NR IgG fragments, but not undigested polyclonal antibodies affected the activity of the nitrate reductase associated with plasma membranes. Discrepancy in the action of intact antibodies and fragments obtained after they digestion were interpreted as a consequence of same differences in the ability of those molecules to the penetration through the membrane. Undigested anti-NR antibody have no effect on the nitrate uptake by intact plants, as well as by the right-side plasma membrane vesicles. On the other hand, IgG fragments of polyclonal antibodies abolished almost totally the nitrate uptake in the case of intact seedlings, but have only slight effect on the N03 uptake in plasma membranes. On the basis of above findings, some relations between nitrate uptake and its assimilation inside the cell are suggested. Since IgG fragments only slightly changed the N03 absorption in vesicles whereas the activity of plasmalemma associated nitrate reductase was strongly repressed, we concluded that the PM-NR is not structurally involved in the nitrate transport through the membrane.

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Influence of simazine on nitrate uptake and nitrate reductase activity in Triticum aestivum (L.) and Cucumis sativus (L.) seedlings

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1984.023 ◽

2014 ◽

Vol 53 (2) ◽

pp. 247-256 ◽

Cited By ~ 1

Author(s):

Irena Jarzyńska ◽

Józef Buczek

Keyword(s):

Triticum Aestivum ◽

Nitrate Reductase ◽

Cucumis Sativus ◽

Nitrate Uptake ◽

Reductase Activity ◽

Triticum Aestivum L ◽

Probable Mechanism ◽

Cucumis Sativus L ◽

Photosynthesis Inhibition ◽

Nr Activity

The influence of simazine (2-chloro-4,6-bis(ethylamine)-1,3,5-triazine) on N03- uptake and nitrate reductase (NR) activity in wheat (Triticum aestivum) and cucumber (Cucumis sativus) seedlings was investigated. It was found that at first (up to 12 h after herbicide treatment) simazine enhanced and then inhibited NR activity in the leaves and roots of the tested plants. After seven days of growth in nutrient medium containing 2.5 and 25 µM simazine the herbicide inhibited by 25 and 70 per cent, respectively, NR activity and reduced by 30 per cent in wheat and 50 per cent in cucumber seedlings nitrate uptake. The probable mechanism of NR inhibition by simazin is discussed and it is suggested that the influence of the herbicide on NR activity may not be exclusively connected with photosynthesis inhibition.

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The uptake and accumulation of phosphorous and nitrates and the activity of nitrate reductase in cucumber seedlings treated with PbCl2 or CdCl2

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1988.034 ◽

2014 ◽

Vol 57 (3) ◽

pp. 349-359 ◽

Cited By ~ 17

Author(s):

Marek Burzyński

Keyword(s):

Heavy Metals ◽

Nitrate Reductase ◽

Cucumis Sativus ◽

Nutrient Medium ◽

Nitrate Reductase Activity ◽

Nitrate Uptake ◽

Reductase Activity ◽

Control Level ◽

Lead And Cadmium ◽

Phosphorous Uptake

Treatment of 4-day-old cucumber (Cucumis sativus L.) seedlings with PbCl2 or CdCl2 caused a significant increase in the accumulation of heavy metals by the plants, especially in the roots. The accumulated Pb initially enhanced the uptake of phosphorous after the plants had been transferred to a nutrient medium (6, 24 hrs), but after only 48 Ins the uptake had dropped to below control level. The plants treated with Cd exhibited a constant decreased phosphorous uptake level. The accumulated lead and cadmium also inhibited nitrate uptake and the activity of nitrate reductase. It is suggested that the reason for the decreased nitrate reductase activity lay rather in the lower nitrate uptake than in a direct effect of the heavy metals on the enzyme.

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Nitrate reductase activity and yield of dry mass and protein content in cucumber seedlings supplied with nitrates and ammonium

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1979.037 ◽

2015 ◽

Vol 48 (3) ◽

pp. 465-471 ◽

Cited By ~ 3

Author(s):

J. Buczek ◽

M. Burzyński

Keyword(s):

Nitrate Reductase ◽

Protein Content ◽

Nutrient Solution ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Sole Source ◽

Dry Mass ◽

Inhibitory Effect ◽

Cucumber Seedlings ◽

Nr Activity

The presence of NH4+ ions in a nutrient solution containing NH4NO3 with the K+ ions removed, caused an inhibition of nitrate reductase (NR) activity in cucumber leaves. The lack of K+ in a NaNO3 medium also decreased the NR activity. Addition of K+ to these media suppressed the inhibitory effect of NH4+ and enhanced the NR activity in the leaves of plants growing in NaNO3 nutrient solution. The results suggest that K+ is essential for NO3- reduction, Na+ is less effective in this process, whereas NH4+ ions markedly inhibit NO3- reduction. The protein content and increment of dry mass of cucumber plants grown 10 days with NH4-N as the sole source of nitrogen was significantly lower as compared with NO3-N supplied plants feed with plants feed with both forms of mineral nitrogen (NH4NO3). The results show that cucumber prefers the nitrates, although it can utilize the ammonium form of nitrogen.

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