13C-mixed-triglyceride breath test: Isotope selective non-dispersive infrared spectrometry in comparison to isotope ratio mass spectrometry in volunteers and patients with chronic pancreatitis

2000 ◽  
Vol 118 (4) ◽  
pp. A418
Author(s):  
Christoph Boedeker ◽  
Oliver Goetze ◽  
Benny Geypens ◽  
Anja Luypaerts ◽  
Wolfgang E. Schmidt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chronic Pancreatitis ◽  
Isotope Ratio ◽  
Breath Test ◽  
Isotope Ratio Mass Spectrometry ◽  
Infrared Spectrometry

scholarly journals Application of isotope-selective nondispersive infrared spectrometry (IRIS) for evaluation of [13C]octanoic acid gastric-emptying breath tests: comparison with isotope ratio–mass spectrometry (IRMS)

1997 ◽  
Vol 43 (3) ◽  
pp. 518-522 ◽  
Author(s):  
Peter Schadewaldt ◽  
Bernd Schommartz ◽  
Gregor Wienrich ◽  
Herbert Brösicke ◽  
Ralf Piolot ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gastric Emptying ◽  
Isotope Ratio ◽  
Time Course ◽  
Octanoic Acid ◽  
Isotope Ratio Mass Spectrometry ◽  
Infrared Spectrometry ◽  
Lag Phase ◽  
Diabetic Patients ◽  
Breath Tests

Abstract Suitability of isotope-selective nondispersive infrared spectrometry (IRIS) for evaluation of [13C]octanoic acid gastric-emptying breath test was assessed and compared with standard isotope ratio–mass spectrometry (IRMS). The estimated bias of IRMS and IRIS measurements of baseline-corrected 13CO2exhalation amounted to ± 0.1 and ± 0.6 Δδ values (n = 360), respectively. In breath tests performed on 60 diabetic patients, the gastric emptying parameters were calculated by nonlinear regression analysis of the time course of 13CO2exhalation: half-emptying time (t1/2,breath, 90 ± 39 min), lag phase (tlag,breath, 34 ± 27 min), and gastric emptying coefficient (GEC, 2.9 ± 0.5). A reasonable linear correlation was found between the two methods (y = IRIS, x = IRMS) with respect to Δδ values (y = 0.35 + 0.92x, r = 0.985, Sy|x = ±0.6, n = 1116) and a rather good agreement of the computed gastric emptying parameters was obtained (t1/2,breath: y = 0.99x + 4.06, Sy|x = ±6.3; tlag,breath: y = 0.97x + 0.96, Sy|x = ±3.4; GEC: y = 0.97x − 0.01, Sy|x = ±0.09).

Comparison of Isotope Ratio Mass Spectrometry and Nondispersive Isotope-Selective Infrared Spectroscopy for 13C-Urea Breath Test

1999 ◽  
Vol 94 (5) ◽  
pp. 1203-1208 ◽  
Author(s):  
Vincenzo Savarino ◽  
Giuseppe Sandro Mela ◽  
Patrizia Zentilin ◽  
Giuliana Bisso ◽  
Monica Pivari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Infrared Spectroscopy ◽  
Isotope Ratio ◽  
Breath Test ◽  
Isotope Ratio Mass Spectrometry ◽  
Urea Breath Test ◽  
13C Urea Breath Test

Phenylalanine kinetics in healthy volunteers and liver cirrhotics: implications for the phenylalanine breath test

2002 ◽  
Vol 283 (6) ◽  
pp. E1223-E1231 ◽  
Author(s):  
I. Tugtekin ◽  
U. Wachter ◽  
E. Barth ◽  
H. Weidenbach ◽  
D. A. Wagner ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liver Function ◽  
Isotope Ratio ◽  
Breath Test ◽  
Isotope Ratio Mass Spectrometry ◽  
Gas Chromatography Mass Spectrometry ◽  
Blood Samples ◽  
Tracer Distribution ◽  
Cirrhotic Patients ◽  
The Impact

Expired 13CO2recovery from an oral l-[1-13C]phenylalanine ([13C]Phe) dose has been used to quantify liver function. This parameter, however, does not depend solely on liver function but also on total CO2 production, Phe turnover, and initial tracer distribution. Therefore, we evaluated the impact of these factors on breath test values. Nine ethyl-toxic cirrhotic patients and nine control subjects received intravenously 2 mg/kg of [13C]Phe, and breath and blood samples were collected over 4 h. CO2 production was measured by indirect calorimetry. The exhaled 13CO2 enrichments were analyzed by isotope ratio mass spectrometry and the [13C]Phe and l-[1-13C]tyrosine enrichments by gas chromatography-mass spectrometry. The cumulative13CO2 recovery was significantly lower in cirrhotic patients (7 vs. 12%; P < 0.01), in part due to lower total CO2 production rates. Phe turnover in cirrhotic patients was significantly lower (33 vs. 44 μmol · kg−1 · h−1; P < 0.05). When these extrahepatic factors were considered in the calculation of the Phe oxidation rate, the intergroup differences were even more pronounced (3 vs. 7 μmol · kg−1 · h−1) than those for 13CO2 recovery data. Also, the Phe-to-Tyr conversion rate, another indicator of Phe oxidation, was significantly reduced (0.7 vs. 3.0 μmol · kg−1 · h−1).

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