In Vivo Immunohistochemistry With Labeled Bevacizumab Using Confocal Laser Endomicroscopy in Models of Human Colorectal Cancer

2011 ◽  
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Ralf Kiesslich ◽  
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Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel imaging technique for gastrointestinal endoscopy providingin vivomicroscopy at subcellular resolution. It offers the possibility to analyze neoangiogenesis and vessel densityin vivo. Angiogenetic switch is essential in cancer progression. Aim of the paper was to review the use of this imaging tool to analyze colorectal and gastric cancers vascularizationin vivo. The aim is to provide the possibility of combining diagnostic evidences with vascularization and molecular profile to evaluate the efficacy of an antiangiogenic treatment in association with conventional therapy. pCLE can be considered a revolutionary method for real-time assessment of changes in vascularization pattern in this tumors and it may open the possibility to address the use of anti-angiogenic therapy in order to improve the outcome of the treatment.


2010 ◽  
Vol 138 (5) ◽  
pp. S-513-S-514
Author(s):  
Sanne Gulikers ◽  
Eveline Rondagh ◽  
Ann Driessen ◽  
Ad Masclee ◽  
Silvia Sanduleanu

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