The effects of phospholipase A2, free fatty acids and lysophosphatidylcholine on the crystalization of cholesterol in gallbladder bile

1998 ◽  
Vol 114 ◽  
pp. A540
Author(s):  
A. Schöne ◽  
D. Jüngst ◽  
T. Hermandez-Richter ◽  
S. Fischer
Keyword(s):  
Fatty Acids ◽  
Phospholipase A2 ◽  
Free Fatty Acids ◽  
Gallbladder Bile

Modulation of plasma membrane H+-ATPase from oat roots by lysophosphatidylcholine, free fatty acids and phospholipase A2

1988 ◽  
Vol 74 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Michael Gjedde Palmgren ◽  
Marianne Sommarin ◽  
Peter Ulvlskov ◽  
Peter Leth Jorgensen
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Phospholipase A2 ◽  
Free Fatty Acids

Hexachlorocyclohexanes affect the arachidonic acid release from phosphatidylinositol but not from other phospholipid classes in tubular cell cultures

1995 ◽  
Vol 15 (4) ◽  
pp. 191-199 ◽  
Author(s):  
J. C. Puente-Fraga ◽  
P. López-Aparicio ◽  
S. Senar ◽  
M. N. Recio ◽  
M. A. Pérez-Albarsanz
Keyword(s):  
Fatty Acids ◽  
Phospholipase A2 ◽  
Free Fatty Acids ◽  
Arachidonic Acid Release ◽  
Phospholipase Activity ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Phospholipid Classes ◽  
Acid Release ◽  
Renal Tubular

Gamma- and delta-isomers of hexachlorocyclohexane caused marked decreases in the levels of radioactive phospholipids, and increases in the levels of [3H]arachidonate incorporated into free fatty acids in rat renal tubular cells. The increased radioactivity of free fatty acids arises from the decrease of [3H]arachidonate incorporated into phosphatidylinositol, but not into phosphatidylcholine, phosphatidylserine or phosphatidylethanolamine. This fact suggests that phosphatidylinositol can be broken down to the fatty acid from the sn-2 position and lysophospholipid by a phospholipase activity increased by hexachlorocyclohexanes. The observed specific toxicant action could be achieved in two ways: (a) operating upon a specific phospholipase A2 that acts on phosphatidylinositol, but not on other phospholipids as substrates and/or (b) involving substrate-phospholipase A2 interactions. Interestingly, the observed effect of the γ-isomer was more pronounced than that of the γ-one.

Activation of Phospholipase Cε by Free Fatty Acids and Cross Talk with Phospholipase D and Phospholipase A2†

Biochemistry ◽  
2006 ◽  
Vol 45 (36) ◽  
pp. 10987-10997 ◽  
Author(s):  
S. N. Prasanna Murthy ◽  
Paul H. Chung ◽  
Li Lin ◽  
Jon W. Lomasney
Keyword(s):  
Fatty Acids ◽  
Phospholipase A2 ◽  
Free Fatty Acids ◽  
Phospholipase D ◽  
Cross Talk ◽  
Phospholipase Cε

Determination of Free Fatty Acids in Human Bile by High-Performance Liquid Chromatography

1998 ◽  
Vol 35 (2) ◽  
pp. 279-282 ◽  
Author(s):  
Yasushi Hori ◽  
Kazutoshi Nakamura ◽  
Masaharu Yamamoto ◽  
Kenji Shimada ◽  
Hiroto Nakadaira ◽  
...  
Keyword(s):  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Free Fatty Acids ◽  
High Performance ◽  
Gallbladder Bile ◽  
Time Saving ◽  
Human Gallbladder ◽  
Human Bile ◽  
The Mean

We developed a high-performance liquid chromatography (HPLC) method for free fatty acids (FFAs) analysis in bile. In this method, FFAs were extracted from bile in a single step using an Isolute™ ODS cartridge, derivatized with 9-anthryldiazomethane (ADAM). ADAM was chosen because of its high reactivity with carboxylic acid at room temperature. Then, HPLC was used for separating and quantifying FFAs. This method proved to be simple and time-saving. The mean recovery of FFA added to human gallbladder bile was 97.6%, and the detection limit was 100–250 pg. Using this method, we determined FFA concentrations in the gallbladder bile of 11 gallstone patients. The mean concentration of total FFA was 0.61 (SD = 0.41) mmol/L, and there was wide variation in the individual FFAs.

Sign in / Sign up

Export Citation Format

Share Document