In vitro evaluation of H. pylori pretreatment antibiotic resistance and evaluation of the disk diffusion test

1998 ◽  
Vol 114 ◽  
pp. A1017
Author(s):  
RG Lahaie ◽  
C Gaudreau ◽  
C Tremblay ◽  
H Gilbert ◽  
S Whittom ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
In Vitro Evaluation ◽  
H Pylori

scholarly journals In Vitro Activities of Fluconazole and Voriconazole against Clinical Isolates of Candida spp. Determined by Disk Diffusion Testing in Turin, Italy

2009 ◽  
Vol 53 (4) ◽  
pp. 1657-1659 ◽  
Author(s):  
Narcisa Mandras ◽  
Vivian Tullio ◽  
Valeria Allizond ◽  
Daniela Scalas ◽  
Giuliana Banche ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Disk Diffusion ◽  
In Vitro Activity ◽  
High Susceptibility ◽  
Diffusion Test ◽  
Candida Spp ◽  
Disk Diffusion Test ◽  
Disk Diffusion Testing ◽  
Fluconazole Resistant

ABSTRACT The in vitro activities of fluconazole and voriconazole against 1,024 clinical isolates of Candida spp. were determined by the agar disk diffusion test using the Clinical and Laboratory Standards Institute (CLSI) M44-A guidelines. The results of this investigation demonstrated the broad-spectrum in vitro activity of voriconazole, relative to that of fluconazole, against yeasts tested, in particular fluconazole-resistant isolates, such as Candida krusei that showed high susceptibility to voriconazole. The situation in Turin, Italy, is quite similar to that of the rest of Italy, reflecting the worldwide trend.

CI-934, a new difluoroquinolone: In vitro antibacterial activity and proposed disk diffusion test interpretive criteria

1987 ◽  
Vol 6 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Peter C. Fuchs ◽  
Arthur L. Barry ◽  
Ronald N. Jones ◽  
Clyde Thornsberry ◽  
Leona W. Ayers ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
In Vitro Antibacterial Activity

scholarly journals Performance of vancomycin and teicoplanin disk diffusion test in isogenic vancomycin non-susceptible Staphylococcus aureus

2015 ◽  
Vol 9 (02) ◽  
pp. 157-164 ◽  
Author(s):  
Sujintana Wongthong ◽  
Karnjana Dutchanutouch ◽  
Viladda Namsaengkang ◽  
Aroonwadee Chanawong ◽  
Chotechana Wilailuckana ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Predictive Value ◽  
Population Analysis ◽  
Area Under The Curve ◽  
Serial Passage ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Methicillin Resistant

Introduction: Detection of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is currently problematic. Although the population analysis profile with area under the curve (PAP-AUC) is the gold standard for detecting hVISA strains, this method is time consuming. This study aimed to induce vancomycin non-susceptible Staphylococcus aureus isolates in methicillin-resistant S. aureus (MRSA) and to determine the performance of the vancomycin and teicoplanin disk diffusion test for screening of induced and natural vancomycin non-susceptible isolates. Methodology: Vancomycin resistance was induced in vitro in methicillin-resistant S. aureus by serial passage in media with increasing vancomycin concentrations. All test isolates were confirmed for their susceptibility to vancomycin by using a PAP-AUC method. The performance of the vancomycin and teicoplanin disk diffusion test for detecting both induced and natural hVISA/VISA isolates was analyzed using the MedCal program version 10.2.0. Results: The induction test revealed that 42 of 78 MRSA isolates (53.8%) became hVISA and/or VISA. Using 10, 15, 20, 30 µg vancomycin disks and a 30 µg teicoplanin disk, the highest performance (88.9%) for hVISA/VISA detection (71.1%, sensitivity, 100% specificity, 100% positive predictive value, and 75% negative predictive value) was obtained when a 20 µg vancomycin disk was used at 1.0 McFarland inoculum for a 24-hour incubation. Conclusions: The results indicated that using a 20 µg vancomycin disk and bacterial inoculum of 1.0 McFarland is simple to perform and provides a primary result for hVISA/VISA screening within 24 hours.

Multicenter Study to Assess the in vitro Activity of Tigecycline by Disk Diffusion Test against Clinical Isolates from Argentina

Chemotherapy ◽  
2009 ◽  
Vol 55 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Liliana Fernández Canigia ◽  
Sara Kaufman ◽  
Liliana Lanata ◽  
Carlos Vay ◽  
Marta Giovanakis ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Disk Diffusion ◽  
In Vitro Activity ◽  
Diffusion Test ◽  
Disk Diffusion Test

scholarly journals Epidemiology and antibiotic succeptibility profile of methicillin sensitive Staphylococcus aureus among livestock and pet animals

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Muhammad Aamir Naseer ◽  
Amjad Islam Aqib ◽  
Muhammad Shoaib ◽  
Iqra Muzammil ◽  
Zeeshan Ahmad Bhutta ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Disk Diffusion ◽  
Diffusion Test ◽  
Disk Diffusion Test ◽  
Zoonotic Pathogen ◽  
Caprine Milk ◽  
Microbiological Examination ◽  
Amoxicillin Clavulanic Acid

Staphylococcus aureus is an important zoonotic pathogen that is responsible for a variety of infectious diseases in humans and animals. The present study was designed to check the prevalence and antimicrobial resistance of MSSA from three different animal origins (bovine, caprine and pet). A total of n= 450 samples (150 each source) were collected from bovine, caprine and pets. Collected samples were subjected to S. aurues identification by microbiological examination and confirmed S. aurues isolates were put to oxacillin disk diffusion test to declare them MSSA. The MSSA confirmed isolates were subjected to various antibiotics for susceptibility profiling using Kirby Baur Disk Diffusion test. The present study found higher prevalence of MSSA from caprine origin (goat 83.33%) as compared to pet (cat 69.33%; dog 65.33%) and bovine origin (buffalo 26.66%; cattle 31.66%). The in-vitro findings of current study revealed oxytetracycline and gentamicin presented 100% efficacy against MSSA of all origins while the vancomycin presented >35%, >40% and > 65% resistance against MSSA isolated from bovine, caprine and pet origin respectively. However, ciprofloxacin was equally effective (50%) against MSSA from buffalo and cattle while >80% efficacy was noted against MSSA from cat and dogs. Linezolid and amoxicillin+ clavulanic acid were 77.78% and 66.67% sensitive to MSSA isolates from caprine milk. The present study found higher prevalence of MSSA from bovine, caprine and pet isolates with diversified pattern of susceptibility of different antibiotics from all sources.

scholarly journals Successful therapy in unusual generalized Dermatophilus congolensis infection in a calf based on modified in vitro disk diffusion test

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Paulo Francisco Domingues ◽  
Simony Trevizan Guerra ◽  
Carolina Lechinski de Paula ◽  
Ana Carolina Alves ◽  
Carmen Alicia Daza Bolanos ◽  
...  
Keyword(s):  
Present Report ◽  
Disk Diffusion ◽  
Successful Therapy ◽  
Diffusion Test ◽  
In Vitro Susceptibility ◽  
Disk Diffusion Test ◽  
First Choice ◽  
Bovine Dermatophilosis ◽  
Long Acting

ABSTRACT: Bovine dermatophilosis is a dermatitis characterized by typical focal or localized lesions with “paintbrush” aspect and occasionally as disseminated cutaneous disease. We report the case of a one-year-old Nelore female with history of chronic cutaneous disseminated lesions that appeared immediately after a rainfall period. Serous to purulent exudates, hair with tufted appearance, hyperkeratotic, non-pruritic, hardened, yellowish to brown, and coalescent crusty lesions were observed distributed all over its body. Removal of the crusts revealed ulcerated or hemorrhagic areas, with irregular elevated crusts like “paintbrush”. Microbiological diagnosis enabled the identification of a microorganism, the Dermatophilus congolensis. Despite disseminated and chronic lesions, we obtained a successful therapy with parenteral therapy using long-acting tetracycline based on modified in vitro disk diffusion test. The present report highlights success therapy in uncommon generalized bovine dermatophilosis with selection of first-choice drugs based on modified in vitro susceptibility test, and need of responsible use of antimicrobials in livestock.

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