Mucosal staining of the esophagus with lugol's iodine solution: Technical aspects

1995 ◽  
Vol 41 (4) ◽  
pp. 352 ◽  
Author(s):  
JA Kidwell ◽  
DE Fleischer ◽  
GQ Wang ◽  
RM Anderson ◽  
B Zhou ◽  
...  
Keyword(s):  
Iodine Solution ◽  
Technical Aspects ◽  
Lugol’S Iodine

scholarly journals Gastric Mucosal Changes Caused by Lugol's Iodine Solution Spray: Endoscopic Features of 64 Cases on Screening Esophagogastroduodenoscopy

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Daisuke Tsurumaru ◽  
Takashi Utsunomiya ◽  
Shuji Matsuura ◽  
Masahiro Komori ◽  
Satoshi Kawanami ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Gastric Body ◽  
Gastric Disease ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Reticular Pattern ◽  
Before And After ◽  
Mucosal Changes ◽  
Greater Curvature ◽  
Gastric Mucosal Changes

Aim. To clarify the endoscopic mucosal change of the stomach caused by Lugol's iodine solution spray on screening esophagogastroduodenoscopy (EGD).Methods. Sixty-four consecutive patients who underwent EGD for esophageal squamous cell carcinoma screening were included in this study. The records for these patients included gastric mucosa findings before and after Lugol's iodine solution was sprayed. The endoscopic findings of the greater curvature of the gastric body were retrospectively analyzed based on the following findings: fold thickening, exudates, ulcers, and hemorrhage.Results. Mucosal changes occurred after Lugol's solution spray totally in 51 patients (80%). Fold thickening was observed in all 51 patients (80%), and a reticular pattern of white lines was found on the surface of the thickened gastric folds found in 28 of the patients (44%). Exudates were observed in 6 patients (9%).Conclusion. The gastric mucosa could be affected by Lugol's iodine; the most frequent endoscopic finding of this effect is gastric fold thickening, which should not be misdiagnosed as a severe gastric disease.

scholarly journals Sequential Application of Lugol’s Iodine Test after Acetic Acid for Detecting Cervical Dysplasia: A Prospective Cohort Study

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1598
Author(s):  
Günther A. Rezniczek ◽  
Samira Ertan ◽  
Sadia Rehman ◽  
Clemens B. Tempfer
Keyword(s):  
Acetic Acid ◽  
Clinical Management ◽  
Prospective Cohort ◽  
Staining Intensity ◽  
Study Endpoint ◽  
Primary Study ◽  
Low Grade ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Iodine Test

Applying Lugol’s iodine solution to the cervix followed by colposcopic assessment is an established standard test to identify low grade/high grade squamous intraepithelial lesions (LSIL/HSIL). Here, we assessed the performance of Lugol’s iodine test during colposcopy using a standardized protocol with 5% acetic acid followed by 5% Lugol’s iodine solution and recording the most severe acetowhite (MSAWL) and iodine-negative (MSINL) lesions in a prospective cohort of consecutive women referred to our specialized colposcopy unit. The primary study endpoint was the sensitivity/specificity of MSINL for the detection of LSIL/HSIL. Secondary endpoints were the time to first appearance of the MSINL, MSINL staining intensity, and fading of MSINL. Three hundred and twenty women were included. The sensitivity and specificity of MSINL for the detection of LSIL/HSIL was 81.4 (95%—confidence interval (CI) 77.3–85.0)% and 29.5 (24.2–35.5)%, respectively. Ninety-six MSINL were identified exclusively by Lugol’s iodine test (no pathology, n = 46; LSIL, n = 29; HSIL, n = 21) (number needed to biopsy to identify one additional LSIL/HSIL = 1.9). In 17/320 (5.3%) patients, the clinical management was changed based on the result of Lugol’s iodine test. Video analysis showed an instant appearance of the MSINL within 10 s in 100% of cases. Intensity of MSINL significantly correlated with the presence/absence of LSIL/HSIL (Spearman rank order correlation; p < 0.0001). Fading of iodine-induced staining intensity over time was not observed. Thus, Lugol’s iodine showed moderate sensitivity and poor specificity, but changed clinical management in 5% of cases when used in addition to acetic acid.

scholarly journals Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

2021 ◽  
Vol 14 (4) ◽  
pp. 873-877
Author(s):  
Juan Carlos Pinilla ◽  
Andrea Isabel Pinilla ◽  
Angel Alberto Florez
Keyword(s):  
Saline Solution ◽  
Direct Method ◽  
Good Recovery ◽  
Iodine Solution ◽  
Direct Examination ◽  
Fecal Samples ◽  
Balantidium Coli ◽  
Lugol’S Iodine ◽  
Fair Degree ◽  
Backyard Pigs

Background and Aim: Balantidium coli is a protozoan that can infect humans and non-human primates, being the domestic pigs the animals most affected by this parasite. This study aimed to compare the performance of five coprological methods for diagnosis of Balantidium coli cysts in fecal samples from pigs. Materials and Methods: From September to December 2019, 558 fecal samples were collected from the rectum of backyard pigs in the Bucaramanga metropolitan area, Colombia. The average age of the sampled animals was 3-4 months. Fecal samples collected were tested using the following coprological techniques: Direct examination with Lugol's iodine solution, buffered saline, centrifugation/flotation and McMaster techniques, and Ziehl–Neelsen method. Results: The results indicate that B. coli cysts were diagnosed most frequently through direct examination with Lugol's iodine solution (52.7% of the samples) followed by direct examination with a buffered saline solution (37.6%). Moderate concordance (k=0.41; p<0.05) was determined between direct method with Lugol's iodine and buffered saline solution, McMaster and buffered saline (k=0.35; p<0.05), and centrifugation/flotation and buffered saline (k=0.28; p<0.05) showed a fair degree of concordance. The rest of the comparisons were classified as poor. The flotation techniques (centrifugation/ flotation and McMaster) did not show good recovery of cysts. Conclusion: It is concluded that the most efficient method for diagnosing this parasitosis was to an association between direct examination with Lugol's iodine solution and buffered saline.

scholarly journals Improved Methodology for Identification of Protists and Microalgae from Plankton Samples Preserved in Lugol's Iodine Solution: Combining Microscopic Analysis with Single-Cell PCR

2008 ◽  
Vol 74 (8) ◽  
pp. 2505-2510 ◽  
Author(s):  
Barbara M. Auinger ◽  
Karin Pfandl ◽  
Jens Boenigk
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Sodium Thiosulfate ◽  
Proteinase K ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Single Cell Pcr ◽  
Field Samples

ABSTRACT Here we introduce a method for quantitative analysis of planktonic protists and microalgae from preserved field samples combining morphological and small-subunit (SSU) rRNA gene sequence analysis. We linked a microscopic screening with PCR of single cells using field samples preserved with Lugol's iodine solution. Cells possessing a rigid cell wall were incubated with Viscozyme and subsequently with proteinase K for cell disruption; this was unnecessary for fragile cells. The addition of sodium thiosulfate to the PCR tube considerably decreased the inhibiting effect of the fixative (iodine) on the PCR and thus allowed for successful single-cell PCR even of long DNA fragments (up to as many as 3,000 base pairs). We further applied the protocol to investigate the dominant SSU rRNA genotypes in distinct flagellate morphospecies originating from different samples. We hypothesized that despite the morphological similarity, protist morphospecies in different habitats or sampled during different seasons are represented by different genotypes. Our results indicate species-specific differences: the two species Ochromonas sp. and Dinobryon divergens were represented by several different genotypes each, and for the latter species, the dominating genotype differed with habitat. In contrast, Dinobryon pediforme, Dinobryon bavaricum, and Synura sphagnicola were exclusively represented by a single genotype each, and the respective genotype was the same in different samples. In summary, our results highlight the significance of molecular variation within protist morphospecies.

scholarly journals Prevalence of Blastocystis hominis and Giardia lamblia Parasites in Patients of Four Regions in East – South Baghdad

2011 ◽  
Vol 35 (2) ◽  
pp. 74-84
Author(s):  
Shatha Abdul Wahab Raof
Keyword(s):  
Methylene Blue ◽  
Giardia Lamblia ◽  
Statistical Significance ◽  
Iodine Solution ◽  
Blastocystis Hominis ◽  
Lugol’S Iodine ◽  
Stool Specimens ◽  
Males And Females ◽  
Medical Importance ◽  
Age Category

A total of 250 stool specimens from patients live in Al-Ameen, Al-Mashtal, Al-Baladiyat and Al-Nahrawan, districts of Baghdad city were collected. The watery and loose specimens were wet mounted with normal saline and buffered methylene blue then all specimens concentrated and examined with Lugol's iodine solution. The protozoal parasites of medical importance Blastocystis hominis and Giardia lamblia were respectively detected in 59 (24.6%) and 42 (17.5%), samples with mixed infection were in eight samples. Differences due to district lacked significance. Statistical significance was observed when the influence of age category was tested (P<0.05). The highest infestation percentage with both parasites was noticed in the 5 – 10 years category whereas the lowest percentage was in the 19 – above years category. Seasonal variations and sex of the infected patients with both protozoa did not reach statistical significance. Significant differences were observed between number of infected patients with B. hominis and G. lamblia in the age categories of respectively Al-Ameen and Al-Baladiyat districts. Significance was recorded in the differences between percentages of the age categories' females as well as of the age category 5 – 10 years males and females that were infected with G. lamblia Similar significance marked the percentages of infection of both sexes with B hominis in the age categories

scholarly journals The effects of formalin and Lugol's iodine solution on protozoal cell volume

Limnologica ◽  
2000 ◽  
Vol 30 (1) ◽  
pp. 59-63 ◽  
Author(s):  
G.M. Zinabu ◽  
Thomas L. Bott
Keyword(s):  
Cell Volume ◽  
Iodine Solution ◽  
Lugol’S Iodine

scholarly journals Qualitative tests for the determination of fraud in raw milk: evaluation of the influence of analytical parameters of the tests and the stability of the samples as a function of time and preservation form

2021 ◽  
Vol 10 (11) ◽  
pp. e450101119860
Author(s):  
Juliana de Araujo ◽  
Cláudia Andréia Gräff ◽  
Wendel Paulo Silvestre
Keyword(s):  
Hydrogen Peroxide ◽  
Storage Time ◽  
Detection Method ◽  
Raw Milk ◽  
Iodine Solution ◽  
False Negatives ◽  
Lugol’S Iodine ◽  
The Stability ◽  
Stability Time

The present work aimed to evaluate the robustness of qualitative tests of frauds of addition of chlorine/hypochlorite, hydrogen peroxide and starch in raw milk, which parameters have the greatest influence on these analytical methods, and to evaluate the influence of time and mode of preservation of intentionally adulterated samples. The effects of the variation of some parameters of the analytical procedures were evaluated, making combinations between the different factors, determining the degree of influence of these variations on the tests. The stability time of the frauds in the samples was evaluated, keeping them under refrigeration (3±2 °C) and carrying out the tests on the same day, and under freezing (≤ -18 °C) for 5, 9 and 15 days, later analyzed, verifying if the frauds were detected. According to the results, it could be observed that the chlorine/hypochlorite detection method was adequate, detecting the addition of the adulterant in all combinations of variables and in all forms of conservation of the samples. The method for detecting the addition of hydrogen peroxide proved to be adequate for the samples analyzed immediately (< 24 h), detecting the presence of the substance in all combinations. However, the method did not detect the presence of peroxide in the combinations for the frozen samples, indicating that the method can generate false negatives in samples that are not fresh. The starch detection method did not detect the presence of this contaminant in all combinations of variables. The combinations that used only a drop of the Lugol’s iodine solution showed false negatives, so the amount of added solution is a critical variable for the method. However, for the other combinations, the method proved to be adequate for both fresh and frozen samples, detecting the addition of starch, regardless of storage time.

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