scholarly journals Comparison between five coprological methods for the diagnosis of Balantidium coli cysts in fecal samples from pigs

2021 ◽  
Vol 14 (4) ◽  
pp. 873-877
Author(s):  
Juan Carlos Pinilla ◽  
Andrea Isabel Pinilla ◽  
Angel Alberto Florez
Keyword(s):  
Saline Solution ◽  
Direct Method ◽  
Good Recovery ◽  
Iodine Solution ◽  
Direct Examination ◽  
Fecal Samples ◽  
Balantidium Coli ◽  
Lugol’S Iodine ◽  
Fair Degree ◽  
Backyard Pigs

Background and Aim: Balantidium coli is a protozoan that can infect humans and non-human primates, being the domestic pigs the animals most affected by this parasite. This study aimed to compare the performance of five coprological methods for diagnosis of Balantidium coli cysts in fecal samples from pigs. Materials and Methods: From September to December 2019, 558 fecal samples were collected from the rectum of backyard pigs in the Bucaramanga metropolitan area, Colombia. The average age of the sampled animals was 3-4 months. Fecal samples collected were tested using the following coprological techniques: Direct examination with Lugol's iodine solution, buffered saline, centrifugation/flotation and McMaster techniques, and Ziehl–Neelsen method. Results: The results indicate that B. coli cysts were diagnosed most frequently through direct examination with Lugol's iodine solution (52.7% of the samples) followed by direct examination with a buffered saline solution (37.6%). Moderate concordance (k=0.41; p<0.05) was determined between direct method with Lugol's iodine and buffered saline solution, McMaster and buffered saline (k=0.35; p<0.05), and centrifugation/flotation and buffered saline (k=0.28; p<0.05) showed a fair degree of concordance. The rest of the comparisons were classified as poor. The flotation techniques (centrifugation/ flotation and McMaster) did not show good recovery of cysts. Conclusion: It is concluded that the most efficient method for diagnosing this parasitosis was to an association between direct examination with Lugol's iodine solution and buffered saline.

scholarly journals Comparison of five parasitological techniques for laboratory diagnosis of Balantidium coli cysts

2016 ◽  
Vol 25 (3) ◽  
pp. 286-292 ◽  
Author(s):  
Alynne da Silva Barbosa ◽  
Otilio Machado Pereira Bastos ◽  
Claudia Maria Antunes Uchôa ◽  
Alcides Pissinatti ◽  
Augusto César Machado Pereira Bastos ◽  
...  
Keyword(s):  
Nonhuman Primates ◽  
Laboratory Diagnosis ◽  
Zoonotic Potential ◽  
Good Recovery ◽  
Direct Examination ◽  
Balantidium Coli ◽  
Sedimentation Technique ◽  
Laboratory Techniques ◽  
Significant Difference ◽  
Examination Techniques

Abstract Balantidium coli is a protozoon that can cause dysentery in humans, pigs and nonhuman primates, with zoonotic potential. In the literature, there is still little information on the effectiveness of different laboratory techniques for diagnosing this disease. This study compared and evaluated the performance of the Lutz, modified Ritchie, Faust, modified Sheather and direct examination techniques for detecting cysts of this protozoon. Between 2012 and 2014, 1905 fecal samples were collected from captive animals in the state of Rio de Janeiro. Of these, 790 were obtained from the rectum of pigs and 1115 from enclosures occupied by nonhuman primates. B. coli cysts were most evident through direct examination (22.4% of the samples) and the Lutz technique (21%). Fair agreement (Kappa = 0.41; p < 0.05) was observed only between direct examination and Lutz. The flotation techniques (Faust and modified Sheather) did not show good recovery of cysts. A statistically significant difference (p < 0.05) in the frequency of cysts between pigs and nonhuman primates could only be observed through direct examination and the Lutz technique. The most efficient method for diagnosing this parasitosis was seen to an association between direct examination and the spontaneous sedimentation technique.

scholarly journals Gastrointestinal Endoparasite Infection on Red Foot (Chelonoidis carbonaria) in Surabaya City Based on Fecal Examination

2021 ◽  
Vol 5 (1) ◽  
pp. 31
Author(s):  
Nurin Ardhiani ◽  
Endang Suprihati ◽  
Aditya Yudhana ◽  
Faisal Fikri
Keyword(s):  
Infectious Disease ◽  
Gastrointestinal Parasites ◽  
Chi Square ◽  
Fecal Examination ◽  
Fecal Samples ◽  
Balantidium Coli ◽  
Chi Square Test

Infectious disease which caused by parasite is the most serious problem in tortoise breeding. This study identifies gastrointestinal endoparasites infection on Red Foot (Chelonoidis carbonaria) during November 2020 to February 2021 in Surabaya City. Fecal samples from a tortoise, a total of 14 tortoises were collected and examined by native, sedimentation and floatation methods for parasitic eggs and oocyst. Fecal samples for this research were collected from breeding farm in Surabaya City. Data obtained from this study were presented descriptively and analyzed by Chi-Square test. Based on examination results, the occurrence of gastrointestinal endoparasite infection on Red foot was recorded at 100% from the total samples. The gastrointestinal parasites were identified as helminths and protozoa namely which is dominately by Tachygonetria spp. (14%), Angusticaecum spp. (3%), and Balantidium coli (2%) respectively. The provision of deworming medication as a curative measure by the owner is carried out regularly and continuously on tortoises that are positively infected with gastrointestinal endoparasites and prevention can be done by paying attention to the management and cleanliness of the cage to prevent potential transmission of endoparasites from the environment to the tortoise.

Mucosal staining of the esophagus with lugol's iodine solution: Technical aspects

1995 ◽  
Vol 41 (4) ◽  
pp. 352 ◽  
Author(s):  
JA Kidwell ◽  
DE Fleischer ◽  
GQ Wang ◽  
RM Anderson ◽  
B Zhou ◽  
...  
Keyword(s):  
Iodine Solution ◽  
Technical Aspects ◽  
Lugol’S Iodine

scholarly journals Gastric Mucosal Changes Caused by Lugol's Iodine Solution Spray: Endoscopic Features of 64 Cases on Screening Esophagogastroduodenoscopy

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Daisuke Tsurumaru ◽  
Takashi Utsunomiya ◽  
Shuji Matsuura ◽  
Masahiro Komori ◽  
Satoshi Kawanami ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Gastric Body ◽  
Gastric Disease ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Reticular Pattern ◽  
Before And After ◽  
Mucosal Changes ◽  
Greater Curvature ◽  
Gastric Mucosal Changes

Aim. To clarify the endoscopic mucosal change of the stomach caused by Lugol's iodine solution spray on screening esophagogastroduodenoscopy (EGD).Methods. Sixty-four consecutive patients who underwent EGD for esophageal squamous cell carcinoma screening were included in this study. The records for these patients included gastric mucosa findings before and after Lugol's iodine solution was sprayed. The endoscopic findings of the greater curvature of the gastric body were retrospectively analyzed based on the following findings: fold thickening, exudates, ulcers, and hemorrhage.Results. Mucosal changes occurred after Lugol's solution spray totally in 51 patients (80%). Fold thickening was observed in all 51 patients (80%), and a reticular pattern of white lines was found on the surface of the thickened gastric folds found in 28 of the patients (44%). Exudates were observed in 6 patients (9%).Conclusion. The gastric mucosa could be affected by Lugol's iodine; the most frequent endoscopic finding of this effect is gastric fold thickening, which should not be misdiagnosed as a severe gastric disease.

scholarly journals Sequential Application of Lugol’s Iodine Test after Acetic Acid for Detecting Cervical Dysplasia: A Prospective Cohort Study

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1598
Author(s):  
Günther A. Rezniczek ◽  
Samira Ertan ◽  
Sadia Rehman ◽  
Clemens B. Tempfer
Keyword(s):  
Acetic Acid ◽  
Clinical Management ◽  
Prospective Cohort ◽  
Staining Intensity ◽  
Study Endpoint ◽  
Primary Study ◽  
Low Grade ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Iodine Test

Applying Lugol’s iodine solution to the cervix followed by colposcopic assessment is an established standard test to identify low grade/high grade squamous intraepithelial lesions (LSIL/HSIL). Here, we assessed the performance of Lugol’s iodine test during colposcopy using a standardized protocol with 5% acetic acid followed by 5% Lugol’s iodine solution and recording the most severe acetowhite (MSAWL) and iodine-negative (MSINL) lesions in a prospective cohort of consecutive women referred to our specialized colposcopy unit. The primary study endpoint was the sensitivity/specificity of MSINL for the detection of LSIL/HSIL. Secondary endpoints were the time to first appearance of the MSINL, MSINL staining intensity, and fading of MSINL. Three hundred and twenty women were included. The sensitivity and specificity of MSINL for the detection of LSIL/HSIL was 81.4 (95%—confidence interval (CI) 77.3–85.0)% and 29.5 (24.2–35.5)%, respectively. Ninety-six MSINL were identified exclusively by Lugol’s iodine test (no pathology, n = 46; LSIL, n = 29; HSIL, n = 21) (number needed to biopsy to identify one additional LSIL/HSIL = 1.9). In 17/320 (5.3%) patients, the clinical management was changed based on the result of Lugol’s iodine test. Video analysis showed an instant appearance of the MSINL within 10 s in 100% of cases. Intensity of MSINL significantly correlated with the presence/absence of LSIL/HSIL (Spearman rank order correlation; p < 0.0001). Fading of iodine-induced staining intensity over time was not observed. Thus, Lugol’s iodine showed moderate sensitivity and poor specificity, but changed clinical management in 5% of cases when used in addition to acetic acid.

scholarly journals Improved Methodology for Identification of Protists and Microalgae from Plankton Samples Preserved in Lugol's Iodine Solution: Combining Microscopic Analysis with Single-Cell PCR

2008 ◽  
Vol 74 (8) ◽  
pp. 2505-2510 ◽  
Author(s):  
Barbara M. Auinger ◽  
Karin Pfandl ◽  
Jens Boenigk
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Sodium Thiosulfate ◽  
Proteinase K ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Iodine Solution ◽  
Lugol’S Iodine ◽  
Single Cell Pcr ◽  
Field Samples

ABSTRACT Here we introduce a method for quantitative analysis of planktonic protists and microalgae from preserved field samples combining morphological and small-subunit (SSU) rRNA gene sequence analysis. We linked a microscopic screening with PCR of single cells using field samples preserved with Lugol's iodine solution. Cells possessing a rigid cell wall were incubated with Viscozyme and subsequently with proteinase K for cell disruption; this was unnecessary for fragile cells. The addition of sodium thiosulfate to the PCR tube considerably decreased the inhibiting effect of the fixative (iodine) on the PCR and thus allowed for successful single-cell PCR even of long DNA fragments (up to as many as 3,000 base pairs). We further applied the protocol to investigate the dominant SSU rRNA genotypes in distinct flagellate morphospecies originating from different samples. We hypothesized that despite the morphological similarity, protist morphospecies in different habitats or sampled during different seasons are represented by different genotypes. Our results indicate species-specific differences: the two species Ochromonas sp. and Dinobryon divergens were represented by several different genotypes each, and for the latter species, the dominating genotype differed with habitat. In contrast, Dinobryon pediforme, Dinobryon bavaricum, and Synura sphagnicola were exclusively represented by a single genotype each, and the respective genotype was the same in different samples. In summary, our results highlight the significance of molecular variation within protist morphospecies.

scholarly journals Prevalence of Blastocystis hominis and Giardia lamblia Parasites in Patients of Four Regions in East – South Baghdad

2011 ◽  
Vol 35 (2) ◽  
pp. 74-84
Author(s):  
Shatha Abdul Wahab Raof
Keyword(s):  
Methylene Blue ◽  
Giardia Lamblia ◽  
Statistical Significance ◽  
Iodine Solution ◽  
Blastocystis Hominis ◽  
Lugol’S Iodine ◽  
Stool Specimens ◽  
Males And Females ◽  
Medical Importance ◽  
Age Category

A total of 250 stool specimens from patients live in Al-Ameen, Al-Mashtal, Al-Baladiyat and Al-Nahrawan, districts of Baghdad city were collected. The watery and loose specimens were wet mounted with normal saline and buffered methylene blue then all specimens concentrated and examined with Lugol's iodine solution. The protozoal parasites of medical importance Blastocystis hominis and Giardia lamblia were respectively detected in 59 (24.6%) and 42 (17.5%), samples with mixed infection were in eight samples. Differences due to district lacked significance. Statistical significance was observed when the influence of age category was tested (P<0.05). The highest infestation percentage with both parasites was noticed in the 5 – 10 years category whereas the lowest percentage was in the 19 – above years category. Seasonal variations and sex of the infected patients with both protozoa did not reach statistical significance. Significant differences were observed between number of infected patients with B. hominis and G. lamblia in the age categories of respectively Al-Ameen and Al-Baladiyat districts. Significance was recorded in the differences between percentages of the age categories' females as well as of the age category 5 – 10 years males and females that were infected with G. lamblia Similar significance marked the percentages of infection of both sexes with B hominis in the age categories

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