Effect of refeeding on IGFI, IGFII, IGF receptors, FGF2, FGF6, and myostatin mRNA expression in rainbow trout myotomal muscle

2003 ◽  
Vol 132 (2) ◽  
pp. 209-215 ◽  
Author(s):  
F. Chauvigné ◽  
J.C. Gabillard ◽  
C. Weil ◽  
P.Y. Rescan
Keyword(s):  
Rainbow Trout ◽  
Mrna Expression ◽  
Igf Receptors ◽  
Myotomal Muscle

Retinol Binding Protein in Rainbow Trout: Molecular Properties and mRNA Expression in Tissues

2001 ◽  
Vol 123 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Marei Sammar ◽  
Patrick J. Babin ◽  
Michéle Durliat ◽  
Iris Meiri ◽  
Itai Zchori ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Mrna Expression ◽  
Binding Protein ◽  
Molecular Properties ◽  
Retinol Binding Protein

Melatonin treatment alters glucosensing capacity and mRNA expression levels of peptides related to food intake control in rainbow trout hypothalamus

2012 ◽  
Vol 178 (1) ◽  
pp. 131-138 ◽  
Author(s):  
Marta Conde-Sieira ◽  
Marta Librán-Pérez ◽  
Marcos A. López Patiño ◽  
José L. Soengas ◽  
Jesús M. Míguez
Keyword(s):  
Rainbow Trout ◽  
Food Intake ◽  
Mrna Expression ◽  
Melatonin Treatment ◽  
Expression Levels ◽  
Mrna Expression Levels ◽  
Intake Control

scholarly journals Chromogranin A, Ki-67 index and IGF-related genes in patients with neuroendocrine tumors

2013 ◽  
Vol 2 (4) ◽  
pp. 172-177 ◽  
Author(s):  
R C S van Adrichem ◽  
L J Hofland ◽  
R A Feelders ◽  
M C De Martino ◽  
P M van Koetsveld ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Neuroendocrine Tumors ◽  
Chromogranin A ◽  
Insulin Receptors ◽  
Proliferation Index ◽  
Gastroenteropancreatic Neuroendocrine Tumors ◽  
Ki 67 ◽  
Igf Binding Proteins ◽  
Igf Receptors ◽  
Igf Binding

Chromogranin A (CgA) and the Ki-67 proliferation index are considered as important biochemical and pathological markers for clinical behaviour of gastroenteropancreatic neuroendocrine tumors (GEP NETs), respectively. The IGF system has been suggested as an important regulator of GEP NET proliferation and differentiation. A possible relationship between serum CgA (sCgA), Ki-67 proliferation index, and expression of IGF-related genes in patients with GEP NETs has not been demonstrated yet. This study investigates the relationship between sCgA, the Ki-67 proliferation index, and the expression of IGF-related genes in GEP NET tissues and their relation with 5-year survival. Tumor and blood samples from 22 GEP NET patients were studied. Tumoral mRNA expression of IGF-related genes (IGFs: IGF1, IGF2; IGF receptors: IGF1R, IGF2R; insulin receptors: subtype A (IR-A) and B (IR-B); IGF-binding proteins (IGFBPs): IGFBP1, IGFBP2, IGFBP3, and IGFBP6) was measured using quantitative RT-PCR. Ki-67 proliferation index was determined using immunohistochemistry. sCgA was measured with ELISA. Five-year survival in patients with nonelevated sCgA (n=11) was 91 vs 46% in patients with elevated sCgA (n=11) (P=0.006). IR-A mRNA expression was significantly higher in tumors obtained from patients with elevated sCgA than in those from patients with nonelevated sCgA (6.42±2.08 vs 2.60±0.40; P=0.04). This data suggests that sCgA correlates well with 5-year survival of GEP NET patients, and that IR-A mRNA expression correlates well with tumor mass in GEP NET patients.

scholarly journals Estrogen receptor mRNA expression patterns in the liver and ovary of female rainbow trout over a complete reproductive cycle

2012 ◽  
Vol 178 (3) ◽  
pp. 556-561 ◽  
Author(s):  
James J. Nagler ◽  
Timothy D. Cavileer ◽  
Joseph S. Verducci ◽  
Irvin R. Schultz ◽  
Sharon E. Hook ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Mrna Expression ◽  
Reproductive Cycle ◽  
Expression Patterns ◽  
Receptor Mrna ◽  
Estrogen Receptor Mrna

In vivo fractional Pi absorption and NaPi-II mRNA expression in rainbow trout are upregulated by dietary P restriction

2003 ◽  
Vol 285 (4) ◽  
pp. R770-R781 ◽  
Author(s):  
Shozo H. Sugiura ◽  
Nichole K. McDaniel ◽  
Ronaldo P. Ferraris
Keyword(s):  
Rainbow Trout ◽  
Tissue Distribution ◽  
Mrna Expression ◽  
Cellular Localization ◽  
Distal Intestine ◽  
Dietary Regulation ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dietary Phosphorus ◽  
Sodium Glucose Cotransporter

Mammalian type II sodium-phosphate cotransporter (NaPi-II) and inorganic phosphate uptake stimulator (PiUS) genes are upregulated by dietary phosphorus (P) restriction to increase intestinal and renal P transport, but little is known about NaPi-II and PiUS regulation in other vertebrates. We studied the 1) the tissue distribution and dietary regulation of NaPi-II, PiUS, and sodium-glucose cotransporter (SGLT1) mRNA and NaPi-II protein in juvenile rainbow trout ( Oncorhynchus mykiss) and 2) effects of dietary P on intestinal Pi absorption in vivo. NaPi-II, PiUS, and SGLT1 mRNA were found in the proximal and distal intestine, pyloric ceca, and kidney. PiUS mRNA was also found in the heart, gill, blood, stomach, liver, skin, and muscle. Tissue distribution of NaPi-II protein correlated with that of NaPi-II mRNA except in gill ionocytes where NaPi-II antibodies recognized related epitopes. Chronic consumption of a low-P diet increased NaPi-II and PiUS but not SGLT1 mRNA abundance in the intestine and kidney. Unlike mammals, there was no detectable shift in tissue or cellular localization of NaPi-II protein in response to dietary P restriction. Regulation of NaPi and PiUS mRNA expression was observed only in fish grown under optimal aqueous oxygen concentrations. In vivo fractional absorption of Pi by the intestine decreased in fish fed high-P diets. Decreases in absorption were less pronounced in fish previously fed low-P diets, suggesting that diet history modulates acute regulation of P absorption. Regulation of dietary Pi absorption in vivo may involve a specific change in intestinal NaPi-II and PiUS gene expression.

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