The influence of phospholipid acyl chain composition on the hepatic lipase-mediated hydrolysis of HDL phospholipids

2000 ◽  
Vol 151 (1) ◽  
pp. 181
Author(s):  
M. Duong ◽  
K.A. Rye
Keyword(s):  
Hepatic Lipase ◽  
Acyl Chain ◽  
Chain Composition ◽  
Acyl Chain Composition ◽  
Phospholipid Acyl Chain ◽  
Hydrolysis Of

scholarly journals The effect of membrane phospholipid acyl-chain composition on the activity of brain-β-N-acetyl-d-glucosaminidase

1981 ◽  
Vol 195 (2) ◽  
pp. 383-388 ◽  
Author(s):  
A Orlacchio ◽  
C Maffei ◽  
L Binaglia ◽  
G Porcellati
Keyword(s):  
Activation Energy ◽  
Membrane Fraction ◽  
Arrhenius Plot ◽  
Acyl Chain ◽  
Membrane Phospholipid ◽  
Chain Composition ◽  
Acyl Chain Composition ◽  
Membrane Bound ◽  
Phospholipid Acyl Chain ◽  
The Relationship

The phospholipid acyl-chain dependence of the membrane-bound lysosomal beta-N-acetyl-D-glucosaminidase has been examined on control membranes from rat brain primary cell cultures and on membrane modified by culturing the cells in media supplemented with polyunsaturated fatty acids. The relationship between beta-N-acetyl-D-glucosaminidase activity and the membrane phospholipid acyl-chain composition has been evaluated. An increase in the unsaturation level of phosphatidyl ethanolamines and phosphatidylcholines, the most abundant phospholipids in this membrane fraction, is related to the rate of the enzymic reaction. The Arrhenius plot of the enzyme activity in modified membranes shows break-temperatures, starting from approximately 15 degrees C. The apparent activation energy below and above the break-temperature is not correlated with phospholipid acyl-chain unsaturation.

scholarly journals The Effect of High Density Lipoprotein Phospholipid Acyl Chain Composition on the Efflux of Cellular Free Cholesterol

1995 ◽  
Vol 270 (11) ◽  
pp. 5882-5890 ◽  
Author(s):  
W. Sean Davidson ◽  
Kristin L. Gillotte ◽  
Sissel Lund-Katz ◽  
William J. Johnson ◽  
George H. Rothblat ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Free Cholesterol ◽  
Acyl Chain ◽  
Density Lipoprotein ◽  
High Density ◽  
Chain Composition ◽  
Acyl Chain Composition ◽  
Phospholipid Acyl Chain

scholarly journals Four Acyltransferases Uniquely Contribute to Phospholipid Heterogeneity in Saccharomyces cerevisiae

2016 ◽  
Vol 9 ◽  
pp. LPI.S40597 ◽  
Author(s):  
Peter Oelkers ◽  
Keshav Pokhrel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acyl Chain ◽  
Wild Type ◽  
Acid Incorporation ◽  
Rich Media ◽  
Chain Composition ◽  
Acyl Chain Composition ◽  
Acyl Chains ◽  
Phospholipid Acyl Chain

Diverse acyl-CoA species and acyltransferase isoenzymes are components of a complex system that synthesizes glycerophospholipids and triacylglycerols. Saccharomyces cerevisiae has four main acyl-CoA species, two main glycerol-3-phosphate 1- O-acyltransferases (Gat1p, Gat2p), and two main 1-acylglycerol-3-phosphate O-acyltransferases (Lpt1p, Slc1p). The in vivo contribution of these isoenzymes to phospholipid heterogeneity was determined using haploids with compound mutations: gat1Δlpt1Δ, gat2Δlpt1Δ, gat1Δslc1Δ, and gat2Δslc1Δ. All mutations mildly reduced [3H]palmitic acid incorporation into phospholipids relative to triacylglycerol. Electrospray ionization tandem mass spectrometry identified few differences from wild type in gat1Δlpt1Δ, dramatic differences in gat2Δslc1Δ, and intermediate changes in gat2Δlpt1Δ and gat1Δslc1Δ. Yeast expressing Gat1p and Lpt1p had phospholipids enriched with acyl chains that were unsaturated, 18 carbons long, and paired for length. These alterations prevented growth at 18.5°C and in 10% ethanol. Therefore, Gat2p and Slc1p dictate phospholipid acyl chain composition in rich media at 30°C. Slc1p selectively pairs acyl chains of different lengths.

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