Cloning and sequencing of cDNA of Sarcophaga peregrina humoral lectin induced on injury of the body wall.

Abstract We have used available chromosomal deficiencies to screen for genetic loci whose zygotic expression is required for formation of body-wall muscle cells during embryogenesis in Caenorhabditis elegans. To test for muscle cell differentiation we have assayed for both contractile function and the expression of muscle-specific structural proteins. Monoclonal antibodies directed against two myosin heavy chain isoforms, the products of the unc-54 and myo-3 genes, were used to detect body-wall muscle differentiation. We have screened 77 deficiencies, covering approximately 72% of the genome. Deficiency homozygotes in most cases stain with antibodies to the body-wall muscle myosins and in many cases muscle contractile function is observed. We have identified two regions showing distinct defects in myosin heavy chain gene expression. Embryos homozygous for deficiencies removing the left tip of chromosome V fail to accumulate the myo-3 and unc-54 products, but express antigens characteristic of hypodermal, pharyngeal and neural development. Embryos lacking a large region on chromosome III accumulate the unc-54 product but not the myo-3 product. We conclude that there exist only a small number of loci whose zygotic expression is uniquely required for adoption of a muscle cell fate.

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Occurrence of a unique fucose-branched chondroitin sulfate in the body wall of a sea cucumber.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)81341-8 ◽

1988 ◽

Vol 263 (34) ◽

pp. 18176-18183 ◽

Cited By ~ 1

Author(s):

R P Vieira ◽

P A Mourão

Keyword(s):

Chondroitin Sulfate ◽

Sea Cucumber ◽

Body Wall ◽

The Body

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Mutations Affecting Nerve Attachment of Caenorhabditis elegans

Genetics ◽

10.1093/genetics/157.4.1611 ◽

2001 ◽

Vol 157 (4) ◽

pp. 1611-1622 ◽

Cited By ~ 1

Author(s):

Go Shioi ◽

Michinari Shoji ◽

Masashi Nakamura ◽

Takeshi Ishihara ◽

Isao Katsura ◽

...

Keyword(s):

Caenorhabditis Elegans ◽

Nerve Cord ◽

Ventral Nerve Cord ◽

Body Wall ◽

The Body ◽

Genetic Loci ◽

Muscle Attachment ◽

C Elegans ◽

Ventral Cord ◽

Excretory Canal

Abstract Using a pan-neuronal GFP marker, a morphological screen was performed to detect Caenorhabditis elegans larval lethal mutants with severely disorganized major nerve cords. We recovered and characterized 21 mutants that displayed displacement or detachment of the ventral nerve cord from the body wall (Ven: ventral cord abnormal). Six mutations defined three novel genetic loci: ven-1, ven-2, and ven-3. Fifteen mutations proved to be alleles of previously identified muscle attachment/positioning genes, mup-4, mua-1, mua-5, and mua-6. All the mutants also displayed muscle attachment/positioning defects characteristic of mua/mup mutants. The pan-neuronal GFP marker also revealed that mutants of other mua/mup loci, such as mup-1, mup-2, and mua-2, exhibited the Ven defect. The hypodermis, the excretory canal, and the gonad were morphologically abnormal in some of the mutants. The pleiotropic nature of the defects indicates that ven and mua/mup genes are required generally for the maintenance of attachment of tissues to the body wall in C. elegans.

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The cuticle of adultNippostrongylus brasiliensis

Parasitology ◽

10.1017/s0031182000068463 ◽

1965 ◽

Vol 55 (1) ◽

pp. 173-181 ◽

Cited By ~ 48

Author(s):

D. L. Lee

Keyword(s):

Electron Microscope ◽

Body Wall ◽

Technical Assistance ◽

Cortical Layer ◽

The Body ◽

Collagen Fibrils ◽

Intestinal Cells ◽

Nippostrongylus Brasiliensis ◽

Normal Appearance

The cuticle of adults ofNippostrongylus brasiliensishas been described using histological, histochemical and ultrastructural techniques.The cuticle has the following layers: an outer triple-layered membrane; a single cortical layer; a fluid-filled layer which is traversed by numerous collagen fibrils; struts which support the fourteen longitudinal ridges of the cuticle and which are suspended by collagen fibrils in the fluid-filled layer; two fibre layers, each layer apparently containing three layers of fibres; and a basement lamella.The fluid-filled layer contains haemoglobin and esterase.The muscles of the body wall are attached to either the basement lamella or to the fibre layers of the cuticle.The mitochondria of the hypodermis are of normal appearance.The longitudinal ridges of the cuticle appear to abrade the microvilli of the intestinal cells of the host.Possible functions of the cuticle are discussed.I wish to thank Dr P. Tate, in whose department this work was done, for helpful suggestions and criticism at all stages of this work, and Mr A. Page for technical assistance. I also wish to thank Professor Boyd for permission to use the electron microscope in the Department of Anatomy.

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