scholarly journals The 5'-flanking region of the mouse vascular smooth muscle alpha-actin gene contains evolutionarily conserved sequence motifs within a functional promoter.

1990 ◽  
Vol 265 (27) ◽  
pp. 16667-16675
Author(s):  
B H Min ◽  
D N Foster ◽  
A R Strauch
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Actin Gene ◽  
Sequence Motifs ◽  
Conserved Sequence ◽  
Evolutionarily Conserved ◽  
Smooth Muscle Alpha Actin ◽  
Conserved Sequence Motifs ◽  
Flanking Region ◽  
Alpha Actin

scholarly journals Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation.

1988 ◽  
Vol 107 (6) ◽  
pp. 2575-2586 ◽  
Author(s):  
D L Ruzicka ◽  
R J Schwartz
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Cardiac Cells ◽  
Actin Gene ◽  
Heart Tube ◽  
Specific Expression ◽  
Actin Genes ◽  
Beta Actin ◽  
Smooth Muscle Alpha Actin ◽  
Alpha Actin

The expression of cytoplasmic beta-actin and cardiac, skeletal, and smooth muscle alpha-actins during early avian cardiogenesis was analyzed by in situ hybridization with mRNA-specific single-stranded DNA probes. The cytoplasmic beta-actin gene was ubiquitously expressed in the early chicken embryo. In contrast, the alpha-actin genes were sequentially activated in avian cardiac tissue during the early stages of heart tube formation. The accumulation of large quantities of smooth muscle alpha-actin transcripts in epimyocardial cells preceded the expression of the sarcomeric alpha-actin genes. The accumulation of skeletal alpha-actin mRNAs in the developing heart lagged behind that of cardiac alpha-actin by several embryonic stages. At Hamburger-Hamilton stage 12, the smooth muscle alpha-actin gene was selectively down-regulated in the heart such that only the conus, which subsequently participates in the formation of the vascular trunks, continued to express this gene. This modulation in smooth muscle alpha-actin gene expression correlated with the beginning of coexpression of sarcomeric alpha-actin transcripts in the epimyocardium and the onset of circulation in the embryo. The specific expression of the vascular smooth muscle alpha-actin gene marks the onset of differentiation of cardiac cells and represents the first demonstration of coexpression of both smooth muscle and striated alpha-actin genes within myogenic cells.

scholarly journals The vascular smooth muscle alpha-actin gene is reactivated during cardiac hypertrophy provoked by load.

1991 ◽  
Vol 88 (5) ◽  
pp. 1581-1588 ◽  
Author(s):  
F M Black ◽  
S E Packer ◽  
T G Parker ◽  
L H Michael ◽  
R Roberts ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Cardiac Hypertrophy ◽  
Actin Gene ◽  
Smooth Muscle Alpha Actin ◽  
Alpha Actin

scholarly journals U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production

2004 ◽  
Vol 24 (4) ◽  
pp. 1769-1778 ◽  
Author(s):  
Vera Atzorn ◽  
Paola Fragapane ◽  
Tamás Kiss
Keyword(s):  
18S Rrna ◽  
Tetrahymena Thermophila ◽  
Sequence Motifs ◽  
Ciliated Protozoan ◽  
Internal Loop ◽  
Conserved Sequence ◽  
Evolutionarily Conserved ◽  
Conserved Sequence Motifs ◽  
Evolutionary Comparison ◽  
Nucleolar Rna

ABSTRACT Saccharomyces cerevisiae snR30 is an essential box H/ACA small nucleolar RNA (snoRNA) required for the processing of 18S rRNA. Here, we show that the previously characterized human, reptilian, amphibian, and fish U17 snoRNAs represent the vertebrate homologues of yeast snR30. We also demonstrate that U17/snR30 is present in the fission yeast Schizosaccharomyces pombe and the unicellular ciliated protozoan Tetrahymena thermophila. Evolutionary comparison revealed that the 3′-terminal hairpins of U17/snR30 snoRNAs contain two highly conserved sequence motifs, the m1 (AUAUUCCUA) and m2 (AAACCAU) elements. Mutation analysis of yeast snR30 demonstrated that the m1 and m2 elements are essential for early cleavages of the 35S pre-rRNA and, consequently, for the production of mature 18S rRNA. The m1 and m2 motifs occupy the opposite strands of an internal loop structure, and they are located invariantly 7 nucleotides upstream from the ACA box of U17/snR30 snoRNAs. U17/snR30 is the first identified box H/ACA snoRNA that possesses an evolutionarily conserved role in the nucleolytic processing of eukaryotic pre-rRNA.

Glue protein genes in Drosophila virilis: their organization, developmental control of transcription and specific mRNA degradation

Development ◽  
1990 ◽  
Vol 108 (2) ◽  
pp. 269-280
Author(s):  
U. Swida ◽  
L. Lucka ◽  
H. Kress
Keyword(s):  
Binding Sites ◽  
Polytene Chromosomes ◽  
Drosophila Virilis ◽  
Ecdysone Receptor ◽  
Sequence Motifs ◽  
Conserved Sequence ◽  
Conserved Sequence Motifs ◽  
Glue Protein ◽  
Flanking Region ◽  
Specific Mrna

The gene Lgp-1, which is localized in the intermoult puff 16A of D. virilis polytene chromosomes, encodes the major larval glue protein Igp-1. The gene consists of two exons interrupted by a short intron. In the 5′ flanking region of Lgp-1, we find putative ecdysone receptor binding sites and two proximal conserved sequence motifs which are possibly involved in gene regulation. The amino acid sequence deduced from the DNA sequence reveals a relationship to the 68C glue protein family of D. melanogaster. The size of the Lgp-1 transcripts decreases in late third instar larvae concomitantly with their disappearance. This is caused by deadenylation followed by distinct nucleolytic attacks in the 3′ untranslated region. Preliminary data suggest the presence of another glue protein gene in the 16A puff region which is related to the Lgp-1 gene.

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