scholarly journals Deaminated neuraminic acid-rich glycoprotein of rainbow trout egg vitelline envelope. Occurrence of a novel alpha-2,8-linked oligo(deaminated neuraminic acid) structure in O-linked glycan chains.

1990 ◽  
Vol 265 (35) ◽  
pp. 21811-21819 ◽  
Author(s):  
A Kanamori ◽  
S Inoue ◽  
M Iwasaki ◽  
K Kitajima ◽  
G Kawai ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Neuraminic Acid ◽  
Vitelline Envelope ◽  
Acid Structure

Immunochemical detection of the major vitelline envelope proteins in the plasma and oocytes of the maturing female rainbow trout, Oncorhynchus mykiss

1992 ◽  
Vol 135 (2) ◽  
pp. 303-NP ◽  
Author(s):  
S. J. Hyllner ◽  
C. Haux
Keyword(s):  
Rainbow Trout ◽  
Total Body Weight ◽  
Envelope Proteins ◽  
Major Protein ◽  
Endocrine Control ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Blot Technique ◽  
Immunochemical Detection ◽  
Vitelline Envelope ◽  
Total Body

ABSTRACT The major vitelline envelope proteins were detected in the plasma of female rainbow trout maturing under natural conditions by using the Western blot technique. Females were sampled every month from July until ovulation in January. The amount of vitelline envelope proteins in plasma increased markedly as the gonads increased in size from 0·4 to about 15% of the total body weight. The plasma level of oestradiol-17β largely followed the alterations in the amount of vitelline envelope proteins, indicating the endocrine control of vitelline envelope protein synthesis. In addition, plasma vitellogenin changed in a manner that resembled the changes in the amount of plasma vitelline envelope proteins. The appearance and growth of the vitelline envelope during oocyte development was demonstrated using immunohistochemical methods. The vitelline envelopes from oocytes at different stages of development were immunoreactive with the antibodies directed against the major vitelline envelope proteins. No immunoreactivity could be observed in the ooplasm or in the surrounding follicular cells, which indicated that the major vitelline envelope proteins were of extraovarian origin. The present study further supports the hypothesis that the major protein constituents of the vitelline envelope in teleosts are under the endocrine control of oestradiol17β and that the site of synthesis is outside the ovary. Journal of Endocrinology (1992) 135, 303–309

Shotgun proteomics of rainbow trout ovarian fluid

2015 ◽  
Vol 27 (3) ◽  
pp. 504 ◽  
Author(s):  
Joanna Nynca ◽  
Georg J. Arnold ◽  
Thomas Fröhlich ◽  
Andrzej Ciereszko
Keyword(s):  
Rainbow Trout ◽  
Cell Structure ◽  
Cell Lineage ◽  
Myeloid Cell ◽  
Shotgun Proteomics ◽  
Oocyte Quality ◽  
Lipid Binding ◽  
Ovarian Fluid ◽  
Proteomic Approach ◽  
Vitelline Envelope

In the present study we used a shotgun proteomic approach to identify 54 proteins of rainbow trout ovarian fluid. The study has unravelled the identity of several proteins not previously reported in fish ovarian fluid. The proteome of trout ovarian fluid consists of diverse proteins participating in lipid binding and metabolism, carbohydrate and ion transport, innate immunity, maturation and ovulation processes. Most trout ovarian fluid proteins correspond to follicular fluid proteins of higher vertebrates, but 15% of the proteins were found to be different, such as those related to the immune system (precerebellin-like protein), proteolysis (myeloid cell lineage chitinase), carbohydrate and lipid binding and metabolism (vitellogenins), cell structure and shape (vitelline envelope protein gamma) and a protein with unknown functions (UPF0762 protein C6orf58 homologue). The present study could help in the decoding of the biological function of these proteins and in the discovery of potential biomarkers of oocyte quality.

Structure of novel gangliosides, deaminated neuraminic acid (KDN) containing glycosphingolipids, isolated from rainbow trout ovarian fluid

Biochemistry ◽  
1993 ◽  
Vol 32 (35) ◽  
pp. 9221-9229 ◽  
Author(s):  
Yu Song ◽  
Ken Kitajima ◽  
Sadako Inoue ◽  
Yutaka Muto ◽  
Takeshi Kasama ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Neuraminic Acid ◽  
Ovarian Fluid

Oestradiol-17β induces the major vitelline envelope proteins in both sexes in teleosts

1991 ◽  
Vol 131 (2) ◽  
pp. 229-236 ◽  
Author(s):  
S. J. Hyllner ◽  
D. O. Oppen-Berntsen ◽  
J. V. Helvik ◽  
B. T. Walther ◽  
C. Haux
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Brown Trout ◽  
Polyclonal Antibodies ◽  
Ovarian Follicle ◽  
Envelope Proteins ◽  
Structural Proteins ◽  
Males And Females ◽  
Vitelline Envelope ◽  
Separate Class

ABSTRACT During growth of the ovarian follicle, the teleost oocyte becomes surrounded by an acellular coat, the vitelline envelope. The nature, origin and number of the vitelline envelope proteins in fish appear to vary with species. In this work, polyclonal antibodies directed against vitelline envelope proteins from rainbow trout, brown trout and turbot were used to show that oestradiol-17β induces the major vitelline envelope proteins in juveniles, both males and females, from different species. The fact that males can synthesize vitelline envelope constituents shows that the origin of these proteins is not confined to the ovary. The vitelline envelope of rainbow trout eggs consists of three major proteins, designated α (60 kDa), β (55 kDa) and γ (50 kDa). The amino acid composition of each of the three proteins indicated that the three proteins are alike and the suggestion that these proteins represent a separate class of structural proteins is sustained. Journal of Endocrinology (1991) 131, 229–236

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