scholarly journals Characterization of a targeting motif for a flagellar membrane protein in Leishmania enriettii.

2002 ◽  
Vol 277 (14) ◽  
pp. 12486
Author(s):  
Erik L. Snapp ◽  
Scott M. Landfear
Keyword(s):  
Membrane Protein ◽  
Flagellar Membrane ◽  
Leishmania Enriettii

scholarly journals Cloning and Characterization of theFlavobacterium johnsoniae Gliding Motility GenesgldD and gldE

2001 ◽  
Vol 183 (14) ◽  
pp. 4167-4175 ◽  
Author(s):  
David W. Hunnicutt ◽  
Mark J. McBride
Keyword(s):  
Membrane Protein ◽  
Cytoplasmic Membrane ◽  
Sequence Similarity ◽  
Gliding Motility ◽  
Wild Type ◽  
Bacteriophage Infection ◽  
Flavobacterium Johnsoniae ◽  
Latex Spheres ◽  
Serovar Typhimurium

ABSTRACT Cells of Flavobacterium johnsoniae move over surfaces by a process known as gliding motility. The mechanism of this form of motility is not known. Cells of F. johnsoniaepropel latex spheres along their surfaces, which is thought to be a manifestation of the motility machinery. Three of the genes that are required for F. johnsoniae gliding motility,gldA, gldB, and ftsX, have recently been described. Tn4351 mutagenesis was used to identify another gene, gldD, that is needed for gliding. Tn4351-induced gldD mutants formed nonspreading colonies, and cells failed to glide. They also lacked the ability to propel latex spheres and were resistant to bacteriophages that infect wild-type cells. Introduction of wild-type gldD into the mutants restored motility, ability to propel latex spheres, and sensitivity to bacteriophage infection. gldD codes for a cytoplasmic membrane protein that does not exhibit strong sequence similarity to proteins of known function. gldE, which lies immediately upstream ofgldD, encodes another cytoplasmic membrane protein that may be involved in gliding motility. Overexpression ofgldE partially suppressed the motility defects of agldB point mutant, suggesting that GldB and GldE may interact. GldE exhibits sequence similarity to Borrelia burgdorferi TlyC and Salmonella enterica serovar Typhimurium CorC.

scholarly journals Functional Expression and Characterization of a Bacterial Light-harvesting Membrane Protein inEscherichia coliand Cell-free Synthesis Systems

2004 ◽  
Vol 68 (9) ◽  
pp. 1942-1948 ◽  
Author(s):  
Yuichiro SHIMADA ◽  
Zheng-Yu WANG ◽  
Yushi MOCHIZUKI ◽  
Masayuki KOBAYASHI ◽  
Tsunenori NOZAWA
Keyword(s):  
Membrane Protein ◽  
Light Harvesting ◽  
Functional Expression

scholarly journals Molecular Cloning and Characterization of theoprQGene Coding for Outer Membrane Protein OprE3 ofPseudomonas aeruginosa

1999 ◽  
Vol 43 (3) ◽  
pp. 297-301 ◽  
Author(s):  
Kiyomi Okamoto ◽  
Naomasa Gotoh ◽  
Hideto Tsujimoto ◽  
Hiroshi Yamada ◽  
Eisaku Yoshihara ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Molecular Cloning ◽  
Outer Membrane Protein
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