Mitochondrial ATP synthase. Quaternary structure of the F1 moiety at 3.6 A determined by x-ray diffraction analysis.

Wild-type and site-specific mutants C166S and C166A (Cys-166 → Ser and Cys-166 → Ala respectively) of the amidase (acylamide amidohydrolase, EC 3.5.1.4) from Pseudomonas aeruginosa were expressed in Escherichia coli by using the vector pKK223-3. Both mutant proteins were catalytically inactive but showed complete cross-reactivity with polyclonal antiserum raised against the wild-type enzyme, as well as CD spectra identical with that of the wild-type enzyme, which were indicative of correct folding. Cys-166 is therefore implicated as the active-site nucleophile. Titration of free thiol groups with 5,5ʹ-dithiobis-(2-nitrobenzoic acid) indicated that Cys-166 is not a rapidly reacting residue. Crystals of both wild-type and C166S amidase grew with identical, rhombohedral morphology; X-ray diffraction analysis established the unit cell dimensions (a = b = c = 84 Å; α = β = γ = 75 °) and space group (R3 or R32). These results imply a quaternary structure of six subunits, with most probably 32 symmetry; the existence of a hexameric structure was supported by molecular mass determinations based on gel filtration and electrophoretic mobility.

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Crystallization of Proteins, Nucleic Acids, and Viruses for X‐ray Diffraction Analysis

Biopolymers Online ◽

10.1002/3527600035.bpol8016 ◽

2003 ◽

Cited By ~ 3

Author(s):

Alexander McPherson ◽

Robert Cudney ◽

Sam Patel

Keyword(s):

Nucleic Acids ◽

Diffraction Analysis ◽

X Ray Diffraction ◽

X Ray

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Electrooptical Effects on Thin Organic Films and X-Ray Diffraction Analysis of 3-Nitroaniline and 2-Cyclo-Octylamino-5-Nitropyridine

10.21236/ada379414 ◽

2000 ◽

Author(s):

Alexander Leyderman ◽

M. Antipin

Keyword(s):

Diffraction Analysis ◽

Organic Films ◽

X Ray Diffraction ◽

X Ray ◽

Thin Organic Films

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Ten-Vertex Polyhedral Monocarbaborane Chemistry. The Novel High-Yield Formation of the Ten-Vertex closo Compound [6-(PMe2Ph)-closo-1-CB9H9] from the Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11]

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19932924 ◽

1993 ◽

Vol 58 (12) ◽

pp. 2924-2935 ◽

Cited By ~ 8

Author(s):

Jane H. Jones ◽

Bohumil Štíbr ◽

John D. Kennedy ◽

Mark Thornton-Pett

Keyword(s):

Nmr Spectroscopy ◽

Diffraction Analysis ◽

High Yield ◽

Monoclinic Space Group ◽

The Novel ◽

X Ray Diffraction ◽

Metal Centre ◽

X Ray ◽

Yield Formation ◽

Boiling Toluene

Thermolysis of [8,8-(PMe2Ph)2-nido-8,7-PtCB9H11] in boiling toluene solution results in an elimination of the platinum centre and cluster closure to give the ten-vertex closo species [6-(PMe2Ph)-closo-1-CB9H9] in 85% yield as a colourles air stable solid. The product is characterized by NMR spectroscopy and single-crystal X-ray diffraction analysis. Crystals (from hexane-dichloromethane) are monoclinic, space group P21/c, with a = 903.20(9), b = 1 481.86(11), c = 2 320.0(2) pm, β = 97.860(7)° and Z = 8, and the structure has been refined to R(Rw) = 0.045(0.051) for 3 281 observed reflections with Fo > 2.0σ(Fo). The clean high-yield elimination of a metal centre from a polyhedral metallaborane or metallaheteroborane species is very rare.

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Bis(μ-carboxylato)dienerhodium(I) Complexes - Synthesis, Characterization and Catalytic Activity

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20081205 ◽

2008 ◽

Vol 73 (8-9) ◽

pp. 1205-1221 ◽

Cited By ~ 4

Author(s):

Jiří Zedník ◽

Jan Sedláček ◽

Jan Svoboda ◽

Jiří Vohlídal ◽

Dmitrij Bondarev ◽

...

Keyword(s):

Catalytic Activity ◽

Diffraction Analysis ◽

Spectroscopic Methods ◽

X Ray Diffraction ◽

X Ray

Dinuclear rhodium(I) η2:η2-cycloocta-1,5-diene (series a) and η2:η2-norborna-2,5-diene (series b) complexes with μ-RCOO- ligands, where R is linear C21H43 (complexes 1a, 1b), CH2CMe3 (2a, 2b), 1-adamantyl (3a, 3b) and benzyl (4a, 4b), have been prepared and characterized by spectroscopic methods. Structures of complexes 2b, 3a and 4a were determined by X-ray diffraction analysis. Complexes prepared show low to moderate catalytic activity in polymerization of phenylacetylene in THF giving high-cis-transoid polymers, but they show only oligomerization activity in dichloromethane.

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