scholarly journals MANOMETRIC DETERMINATION OF PRIMARY AMINO NITROGEN AND ITS APPLICATION TO BLOOD ANALYSIS

1929 ◽  
Vol 83 (2) ◽  
pp. 425-447 ◽  
Author(s):  
Donald D. Van Slyke
Keyword(s):  
Amino Nitrogen ◽  
Blood Analysis ◽  
Primary Amino ◽  
Manometric Determination

scholarly journals Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 164
Author(s):  
Labrini V. Athanasiou ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Panagiotis D. Katsoulos
Keyword(s):  
Borrelia Burgdorferi ◽  
Rural Areas ◽  
Blood Analysis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Igm Antibodies ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Immunofluorescence Antibody

Exposure of sheep to Borreliaburgdorferi sensulato (s.I.) complex, the causative agent of Lyme borreliosis (LB), has been reported in tick-abundant areas worldwide, while no data have been reported in Greece. The aim of the study was to identify the hematological alterations in sheep with seropositivity against Borrelia burgdorferi (s.I.). Blood samples were obtained from 318 tick infested sheep for blood analysis and serological determination of IgG and IgM antibodies against B. burgdorferi by indirect immunofluorescence antibody (IFA) assay after exclusion of endo-ectoparasites and other tick-borne infections. A total number of 162 sheep met the inclusion criteria, allocated in four groups based on the presence or absence of IgG and/or IgM; sheep found negative for IgM and IgG (Group A), positive for IgM (Group B), positive for both IgM and IgG (Group C) and positive for IgG (Group D). Anemia, thrombocytopenia and normal or decreased leukocyte count, mainly due to lymphopenia were the main hematological features observed in seropositive sheep. The presence of these features raises the suspicion of Borrelia infection in tick infested sheep. The seropositivity of 23.58% in sheep raises concerns of Borrelia circulation, especially in rural areas and potential risk of transmission to humans.

scholarly journals Vascular erectile dysfunction: a comprehensive approach to diagnosis

2021 ◽  
Vol 14 (1) ◽  
pp. 68-76
Author(s):  
A.A. Kamalov ◽  
◽  
S.T. Matskeplishvili ◽  
M.E. Chaliy ◽  
D.A. Okhobotov ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Erectile Dysfunction ◽  
Cardiovascular Diseases ◽  
Early Stage ◽  
Cardiovascular Complications ◽  
Blood Analysis ◽  
Biomedical Literature ◽  
Impact Factors ◽  
Scientific Publications

Introduction. Erectile dysfunction (ED) combines many conditions leading to erectile dysfunction and disability to perform sexual intercourse. It was confirmed that the most common cause of ED is vascular disorders, primarily associated with atherosclerosis that leads to cardiovascular diseases. Objective. To analyze the available literature data about the methods for diagnosis vascular ED, including according to cardiovascular system status. Materials and methods. The results of the search in scientific databases Medline, EMBASE, Cochrane, Chinese BioMedical Literature Database, China National Knowledge Infrastructure, Elibrary.ru were analyzed. After a detailed verification of the reliability of sources, impact factors of journals and the sequence of presentation of the material, 63 most relevant scientific publications were selected directly for citation. Results. The first step of the vascular ED diagnosis is the monitoring of nocturnal penile tumescences. Next, the risk factors for cardiovascular diseases and ED are evaluated: anamnesis of elevated blood pressure, physical activity, smoking, body weight, measurement of blood pressure, blood analysis with the lipid and glycemic profiles. For the diagnosis of endothelial dysfunction (END), the determination of flow-dependent vasodilation of the brachial artery is used, with the possible determination of the rate of blood filling after decompression. After the END is confirmed in patients with ED, it is useful to determine the vascular age using the SCORE scale. For further management of the patient with ED and END, stratification of the associated with sexual activity cardiovascular complications risk is recommended. Conclusion. The proposed diagnostic algorithm will allow not only identify the vascular component of ED at an early stage, but also to avoid fatal cardiovascular complications by prescribing and recommending therapeutic and preventive measures to patients with asymptomatic cardiovascular diseases.

Direct Manometric Determination of Vapor Pressure

2001 ◽  
Vol 1 (1) ◽  
pp. A2.4.1-A2.4.6
Author(s):  
M.S. Rahman ◽  
S.S. Sablani ◽  
N. Guizani ◽  
T.P. Labuza ◽  
P.P. Lewiki
Keyword(s):  
Vapor Pressure ◽  
Manometric Determination

High-resolution capillary electrophoresis for the determination of carbamylated albumin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Julien Favresse ◽  
Joris Delanghe
Keyword(s):  
Separation Efficiency ◽  
Theoretical Plate ◽  
Clinical Routine ◽  
Negative Prognostic Factor ◽  
Symmetry Factor ◽  
Primary Amino ◽  
The Mean ◽  
Dose Dependent

Abstract Objectives Carbamylation is a non-enzymatic post-translational reaction of a primary amino group of a protein with isocyanate. The albumin carbamylation is a negative prognostic factor in chronic kidney disease (CKD) patients and induce charge difference implying an observed shift in electrophoretic mobility that can be measured through a symmetry factor (SF). Methods The Helena V8 and the Sebia Capillarys 2 systems were used for all experiments. The effect of in vitro carbamylation on the SF by spiking increasing concentrations of potassium isocyanate (KCNO) in serum of three healthy volunteers was investigated. Theoretical plate numbers (N) as a surrogate of separation efficiency were also calculated and correlations between SF and renal function biomarkers were performed on 284 patients. Results A dose-dependent impact of KCNO on the SF was observed for both methods with the Helena V8 being more sensitive. The mean N was significantly higher on the Helena V8 as compared to the Sebia Capillarys 2 (2,972 vs. 444.1, p<0.0001). The SF correlated significantly with eGFR (r=0.50, p<0.0001), creatinine (r=−0.31, p<0.0001) and urea (r=−0.34, p<0.0001) on the Helena V8. On the Sebia Capillarys 2, a significant correlation was only observed with eGFR (r=0.17, p=0.004). A better discrimination between CKD stages was also observed using the Helena V8. Conclusions Thanks to a higher mean N, the Helena V8 might offer new possibilities, including detection of carbamylated albumin through SF calculation. Further studies are still needed to confirm the interest of using this type of assays in clinical routine.

Validation of methods for determining pediatric midazolam using wet whole blood and volumetric absorptive microsampling

Bioanalysis ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 1737-1754 ◽  
Author(s):  
Paul Abu-Rabie ◽  
Bikalpa Neupane ◽  
Neil Spooner ◽  
James Rudge ◽  
Philip Denniff ◽  
...  
Keyword(s):  
Whole Blood ◽  
Pediatric Patients ◽  
Blood Analysis ◽  
Pharmacokinetic Parameters ◽  
Validation Data ◽  
Bioanalytical Method ◽  
Whole Blood Analysis ◽  
Suitable Technique ◽  
Volumetric Absorptive Microsampling

Aim: Collection and quantitative analysis in dry blood using volumetric absorptive microsampling (VAMS™) potentially offers significant advantages over conventional wet whole blood analysis. This manuscript explores their use for pediatric sampling and explores additional considerations for the validation of the bioanalytical method. Results: HPLC–MS/MS methods for the determination of midazolam and its major metabolite 1-OH midazolam in both whole wet blood, and dry blood collected on VAMS were developed, validated, and used to support an observational clinical study to compare pharmacokinetic parameters in pediatric patients. Conclusion: Validation data met internationally accepted guideline criteria. A strong correlation was observed in calculated concentrations between wet and dry test samples, indicating that VAMS is a suitable technique for use in pediatric clinical studies.

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