scholarly journals Cloning, characterization, and expression of the gene for the catalytic subunit of cAMP-dependent protein kinase in Caenorhabditis elegans. Identification of highly conserved and unique isoforms generated by alternative splicing.

1990 ◽  
Vol 265 (12) ◽  
pp. 6896-6907
Author(s):  
R E Gross ◽  
S Bagchi ◽  
X Lu ◽  
C S Rubin
Keyword(s):  
Alternative Splicing ◽  
Caenorhabditis Elegans ◽  
Protein Kinase ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Organization and alternative splicing of the Caenorhabditis elegans cAMP-dependent protein kinase catalytic-subunit gene (kin-1)

1999 ◽  
Vol 339 (1) ◽  
pp. 209-216
Author(s):  
Mohammad TABISH ◽  
Roger A. CLEGG ◽  
Huw H. REES ◽  
Michael J. FISHER
Keyword(s):  
Alternative Splicing ◽  
Caenorhabditis Elegans ◽  
Protein Kinase ◽  
Structural Diversity ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Gene Encoding ◽  
Exon 2 ◽  
Camp Dependent Protein Kinase ◽  
Dependent Protein

The cAMP-dependent protein kinase (protein kinase A, PK-A) is multifunctional in nature, with key roles in the control of diverse aspects of eukaryotic cellular activity. In the case of the free-living nematode, Caenorhabditis elegans, a gene encoding the PK-A catalytic subunit has been identified and two isoforms of this subunit, arising from a C-terminal alternative-splicing event, have been characterized [Gross, Bagchi, Lu and Rubin (1990) J. Biol. Chem. 265, 6896–6907]. Here we report the occurrence of N-terminal alternative-splicing events that, in addition to generating a multiplicity of non-myristoylatable isoforms, also generate the myristoylated variant(s) of the catalytic subunit that we have recently characterized [Aspbury, Fisher, Rees and Clegg (1997) Biochem. Biophys. Res. Commun. 238, 523–527]. The gene spans more than 36 kb and is divided into a total of 13 exons. Each of the mature transcripts contains only 7 exons. In addition to the already characterized exon 1, the 5´-untranslated region and first intron actually contain 5 other exons, any one of which may be alternatively spliced on to exon 2 at the 5´ end of the pre-mRNA. This N-terminal alternative splicing occurs in combination with either of the already characterized C-terminal alternative exons. Thus, C. elegans expresses at least 12 different isoforms of the catalytic subunit of PK-A. The significance of this unprecedented structural diversity in the family of PK-A catalytic subunits is discussed.

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