scholarly journals Isolation of the soluble substrate recognition component of the dicarboxylate transport system of Escherichia coli.

1975 ◽  
Vol 250 (4) ◽  
pp. 1600-1602 ◽  
Author(s):  
T C Lo ◽  
B D Sanwal
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Substrate Recognition ◽  
Dicarboxylate Transport ◽  
Soluble Substrate

Dicarboxylic acid transport in Escherichia coli K12: involvement of a binding protein in the translocation of dicarboxylic acids across the outer membrane of the cell envelope

1979 ◽  
Vol 57 (6) ◽  
pp. 653-661 ◽  
Author(s):  
Mary A. Bewick ◽  
Theodore C. Y. Lo
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Outer Membrane ◽  
Transport System ◽  
Cell Envelope ◽  
Binding Protein ◽  
Dicarboxylic Acids ◽  
Escherichia Coli K12 ◽  
Dicarboxylate Transport ◽  
Active Transport System

We have previously found that the dicarboxylate transport system in Escherichia coli K12 is an active transport system and that at least one binding protein and two cytoplasmic membrane transport components are involved in the uptake of dicarboxylic acids. Recently, through surface labelling studies, some dicarboxylate binding proteins were found to be exposed on the cell surface. In the present paper, we demonstrate that the dicarboxylate transport component located in the outer membrane can be inactivated by two different kinds of nonpenetrating inhibitors, viz. proteases, and diazosulfanilic acid. These inhibitors seem to act on the dicarboxylate binding protein. By adding this protein to inactivated cells or to transport-negative mutants, we have succeeded in reconstituting the dicarboxylate transport system. These findings suggest that the dicarboxylate binding protein found on the cell surface plays an essential role in the translocation of dicarboxylic acids across the outer membrane.

scholarly journals Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome.

1993 ◽  
Vol 268 (1) ◽  
pp. 146-152 ◽  
Author(s):  
R. Pistocchi ◽  
K. Kashiwagi ◽  
S. Miyamoto ◽  
E. Nukui ◽  
Y. Sadakata ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transport System
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