scholarly journals Molecular cloning of gene sequences for avian fatty acid synthase and evidence for nutritional regulation of fatty acid synthase mRNA concentration.

1982 ◽  
Vol 257 (6) ◽  
pp. 3225-3229
Author(s):  
S M Morris ◽  
J H Nilson ◽  
R A Jenik ◽  
L K Winberry ◽  
M A McDevitt ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Cloning ◽  
Fatty Acid Synthase ◽  
Nutritional Regulation ◽  
Gene Sequences ◽  
Mrna Concentration

scholarly journals Occupancy and Function of the −150 Sterol Regulatory Element and −65 E-Box in Nutritional Regulation of the Fatty Acid Synthase Gene in Living Animals

2003 ◽  
Vol 23 (16) ◽  
pp. 5896-5907 ◽  
Author(s):  
Maria-Jesus Latasa ◽  
Michael J. Griffin ◽  
Yang Soo Moon ◽  
Chulho Kang ◽  
Hei Sook Sul
Keyword(s):  
Fatty Acid ◽  
Binding Sites ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Nutritional Regulation ◽  
Fed State ◽  
E Box ◽  
Sterol Regulatory Element ◽  
E Boxes

ABSTRACT Upstream regulatory factor (USF) and sterol regulatory element binding protein (SREBP) play key roles in the transcriptional regulation of the fatty acid synthase (FAS) gene by feeding and insulin. Due to the dual binding specificity of SREBP, as well as the presence of multiple consensus sites for these transcription factors in the FAS promoter, their physiologically relevant functional binding sites have been controversial. Here, in order to determine the occupancy of the putative USF and SREBP binding sites, we examined their protein-DNA interactions in living animals by using formaldehyde cross-linking and immunoprecipitation of chromatin and tested the function of these elements by employing mice transgenic for a reporter gene driven by various 5′ deletions as well as site-specific mutations of the FAS promoter. We show that the −332 and −65 E-boxes are bound by USF in both fasted and refed mice, while the −150 SRE is bound by SREBP-1 only in refed mice. We also found that mutation of either the −150 SRE or the −65 E-box abolishes the feeding-induced activation of the FAS promoter in transgenic mice. Furthermore, in vivo occupancy of the FAS promoter by SREBP in the fed state can be prevented by mutation not only of the −150 SRE but, unexpectedly, of the −65 E-box as well. We conclude that the FAS promoter is activated during refeeding via the induced binding of SREBP to the −150 SRE and that USF binding to the −65 E-box is also required for SREBP binding and activation of the FAS promoter.

scholarly journals Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase.

1989 ◽  
Vol 86 (9) ◽  
pp. 3114-3118 ◽  
Author(s):  
C. M. Amy ◽  
A. Witkowski ◽  
J. Naggert ◽  
B. Williams ◽  
Z. Randhawa ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Cloning ◽  
Fatty Acid Synthase ◽  
Cloning And Sequencing

scholarly journals Human fatty acid synthase: properties and molecular cloning.

1995 ◽  
Vol 92 (19) ◽  
pp. 8695-8699 ◽  
Author(s):  
A. Jayakumar ◽  
M. H. Tai ◽  
W. Y. Huang ◽  
W. al-Feel ◽  
M. Hsu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Cloning ◽  
Fatty Acid Synthase

Retinoic acid-mediated transcription and maturation of SREBP-1c regulates fatty acid synthase via cis-elements responsible for nutritional regulation

2007 ◽  
Vol 35 (5) ◽  
pp. 1211-1214 ◽  
Author(s):  
K. Roder ◽  
M. Schweizer
Keyword(s):  
Retinoic Acid ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Proximal Promoter ◽  
Nutritional Regulation ◽  
Cis Elements ◽  
Response Elements ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

A region of the rat FAS (fatty acid synthase) promoter has been defined as being responsible for RA (retinoic acid) responsiveness. The defined promoter region is devoid of canonical RA-response elements but contains cis-elements binding generalized and specific transcription factors that mediate the dietary response of FAS. Our results are consistent with SREBP-1c (sterol-regulatory-element-binding protein 1c) binding in this region, thus bringing about the RA responsiveness of the rat FAS proximal promoter.

Discovery of fatty acid synthase inhibitors and their biosynthetic pathways by a novel target-directed genome mining strategy

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
J Li ◽  
X Tang ◽  
JJ Zhang ◽  
EC O'Neill ◽  
SM Mantovani ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Genome Mining ◽  
Biosynthetic Pathways ◽  
Novel Target
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