Dynamic deformation behavior of normal human red blood cells freely suspended in a sinusoidally oscillating quasi-Couette shear flow: Elastic stretching and viscoelastic recovering model

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

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Oxidation-induced calcium-dependent dehydration of normal human red blood cells

Free Radical Research ◽

10.1080/10715760601161452 ◽

2007 ◽

Vol 41 (5) ◽

pp. 536-545 ◽

Cited By ~ 10

Author(s):

Irina M. Shcherbachenko ◽

Irina L. Lisovskaya ◽

Vladimir P. Tikhonov

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Calcium Dependent ◽

Human Red Blood Cells ◽

Normal Human

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Effect of Pyridine Nucleotides on the 1-14C-Glucose Utilization of Normal Human Red Blood Cells

Nature ◽

10.1038/195500b0 ◽

1962 ◽

Vol 195 (4840) ◽

pp. 500-501 ◽

Cited By ~ 6

Author(s):

ROBERT H. HERMAN ◽

JESSE BERKOWITZ ◽

LLOYD E. CLAYTON ◽

JACQUES L. SHERMAN

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Glucose Utilization ◽

Pyridine Nucleotides ◽

Human Red Blood Cells ◽

Normal Human

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Potassium, Sodium, and Water in Normal Human red Blood Cells

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365516609051812 ◽

1966 ◽

Vol 18 (2) ◽

pp. 167-180 ◽

Cited By ~ 57

Author(s):

J. Funder ◽

J. O. Wieth

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Potassium Sodium ◽

Human Red Blood Cells ◽

Normal Human

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Changes in the properties of normal human red blood cells during in vivo aging

American Journal of Hematology ◽

10.1002/ajh.23344 ◽

2012 ◽

Vol 88 (1) ◽

pp. 44-51 ◽

Cited By ~ 46

Author(s):

Robert S. Franco ◽

M. Estela Puchulu-Campanella ◽

Latorya A. Barber ◽

Mary B. Palascak ◽

Clinton H. Joiner ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Human Red Blood Cells ◽

Normal Human

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Role of membrane viscosity in the orientation and deformation of a spherical capsule suspended in shear flow

Journal of Fluid Mechanics ◽

10.1017/s002211208500341x ◽

1985 ◽

Vol 160 ◽

pp. 119-135 ◽

Cited By ~ 96

Author(s):

D. Barthes-Biesel ◽

H. Sgaier

Keyword(s):

Relaxation Time ◽

Shear Flow ◽

Red Blood Cells ◽

Blood Cells ◽

Liquid Droplets ◽

Regular Perturbation ◽

Shear Rates ◽

Freely Suspended ◽

Spherical Capsule

Red blood cells or artificial vesicles may be conveniently represented by capsules, i.e. liquid droplets surrounded by deformable membranes. The aim of this paper is to assess the importance of viscoelastic properties of the membrane on the motion of a capsule freely suspended in a viscous liquid subjected to shear flow. A regular perturbation solution of the general problem is obtained when the particle is initially spherical and undergoing small deformations. With a purely viscous membrane (infinite relaxation time) the capsule deforms into an ellipsoid and has a continuous flipping motion. When the membrane relaxation time is of the same order as the shear time, the particle reaches a steady ellipsoidal shape which is oriented with respect to streamlines at an angle that varies between 45° and 0°, and decreases with increasing shear rates. Furthermore it is predicted that the deformation reaches a maximum value, which is consistent with experimental observations of red blood cells.

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