Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

Oxidation-induced calcium-dependent dehydration of normal human red blood cells

2007 ◽  
Vol 41 (5) ◽  
pp. 536-545 ◽  
Author(s):  
Irina M. Shcherbachenko ◽  
Irina L. Lisovskaya ◽  
Vladimir P. Tikhonov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Calcium Dependent ◽  
Human Red Blood Cells ◽  
Normal Human

Effect of Pyridine Nucleotides on the 1-14C-Glucose Utilization of Normal Human Red Blood Cells

Nature ◽  
1962 ◽  
Vol 195 (4840) ◽  
pp. 500-501 ◽  
Author(s):  
ROBERT H. HERMAN ◽  
JESSE BERKOWITZ ◽  
LLOYD E. CLAYTON ◽  
JACQUES L. SHERMAN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Glucose Utilization ◽  
Pyridine Nucleotides ◽  
Human Red Blood Cells ◽  
Normal Human

scholarly journals Changes in the properties of normal human red blood cells during in vivo aging

2012 ◽  
Vol 88 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Robert S. Franco ◽  
M. Estela Puchulu-Campanella ◽  
Latorya A. Barber ◽  
Mary B. Palascak ◽  
Clinton H. Joiner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Normal Human

Colorimetric Determination of Blood Arginase

1955 ◽  
Vol 1 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Max M Friedman ◽  
Edythe Becker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Colorimetric Determination ◽  
Normal Human ◽  
Colorimetric Procedure

Abstract A colorimetric procedure for blood arginase has been described, based on hemolyzing the red blood cells with saponin and determining the urea formed by action of the enzyme on an arginine substrate. The range of blood arginase in a series of normal human subjects has been presented.

Increase of Enzyme Activities following the in vitro Peroxidation of Normal Human Red Blood Cells

Enzyme ◽  
1988 ◽  
Vol 39 (1) ◽  
pp. 1-7 ◽  
Author(s):  
J. L. Vives Carrons ◽  
M. A. Pujades ◽  
D. Colomer
Keyword(s):  
Red Blood Cells ◽  
Enzyme Activities ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Normal Human

scholarly journals Changes in Haematological Parameters in Plasmodium falciparum Infected Malaria Patients in an Urban Slum of Lagos, Nigeria

2020 ◽  
pp. 20-29
Author(s):  
U. O. Ozojiofor ◽  
O. O. Bankole ◽  
N. Anene ◽  
A. U. Hassan ◽  
S. A. Emaleku
Keyword(s):  
Red Blood Cells ◽  
Parasite Density ◽  
Blood Cells ◽  
Malaria Parasite ◽  
Human Subjects ◽  
White Blood Cells ◽  
Urban Slum ◽  
Haematological Parameters ◽  
Distribution Width ◽  
The Mean

The present study was carried to determine the changes in haematological parameters in P. falciparum infected patients in Ajeromi Ifelodun area of Lagos, Nigeria. Seventy (70) human subjects comprising of 50 P. falciparum malarial infected and 20 non-infected (control) subjects between 10-60 years were selected for this study. RDT test and microscopy were carried out to ascertain the presence of P. falciparum. They were grouped based on age criteria and level of parasitaemia. This work was carried out in the Department of Biochemistry, Nigeria Institute for Medical Research Laboratory, Yaba, Lagos, Nigeria between August 2016 and January 2017. Blood samples were collected for the determination of P. falciparum, level of parasitaemia and haematological parameters. Haematological parameters were determined using a Coulter A-T Pierce haematology analyzer (Beckman Coulter, Inc. Fullerton, CA, USA), P. falciparum was determined by rapid diagnostic test (RDT) and Microscopy. There was a significant increase in the mean level of total white blood cells (WBC) and red blood cells distribution width (RDW), and a significant decrease in the mean level of haematocrit (HCT), haemoglobin (HGB), red blood cells (RBC), and platelets (PLT) in the malaria infected patients than in the controls (p<0.05). There was also a higher malaria parasite density among malaria infected patients for ages above 20 and a lower malaria parasite density for ages below 20 in this study. The findings of this study show that infection with P. falciparum produces changes in haematological parameters in those infected and tested positive for malaria. The most commonly affected parameters are haemoglobin, haematocrit, white blood cells and platelet count.

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