Effect of Pyridine Nucleotides on the 1-14C-Glucose Utilization of Normal Human Red Blood Cells

Nature ◽  
1962 ◽  
Vol 195 (4840) ◽  
pp. 500-501 ◽  
Author(s):  
ROBERT H. HERMAN ◽  
JESSE BERKOWITZ ◽  
LLOYD E. CLAYTON ◽  
JACQUES L. SHERMAN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Glucose Utilization ◽  
Pyridine Nucleotides ◽  
Human Red Blood Cells ◽  
Normal Human

Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

Oxidation-induced calcium-dependent dehydration of normal human red blood cells

2007 ◽  
Vol 41 (5) ◽  
pp. 536-545 ◽  
Author(s):  
Irina M. Shcherbachenko ◽  
Irina L. Lisovskaya ◽  
Vladimir P. Tikhonov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Calcium Dependent ◽  
Human Red Blood Cells ◽  
Normal Human

scholarly journals Changes in the properties of normal human red blood cells during in vivo aging

2012 ◽  
Vol 88 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Robert S. Franco ◽  
M. Estela Puchulu-Campanella ◽  
Latorya A. Barber ◽  
Mary B. Palascak ◽  
Clinton H. Joiner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Normal Human

Increase of Enzyme Activities following the in vitro Peroxidation of Normal Human Red Blood Cells

Enzyme ◽  
1988 ◽  
Vol 39 (1) ◽  
pp. 1-7 ◽  
Author(s):  
J. L. Vives Carrons ◽  
M. A. Pujades ◽  
D. Colomer
Keyword(s):  
Red Blood Cells ◽  
Enzyme Activities ◽  
Blood Cells ◽  
Human Red Blood Cells ◽  
Normal Human

Peptides from the Plasmodium falciparum STEVOR putative protein bind with high affinity to normal human red blood cells

Peptides ◽  
2005 ◽  
Vol 26 (7) ◽  
pp. 1133-1143 ◽  
Author(s):  
Javier E. García ◽  
Alvaro Puentes ◽  
Hernando Curtidor ◽  
Ricardo Vera ◽  
Luis Rodriguez ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Putative Protein ◽  
High Affinity ◽  
Human Red Blood Cells ◽  
Normal Human

scholarly journals Synthesis of Riboflavin Nucleotides by Mature Human Erythrocytes

Blood ◽  
1970 ◽  
Vol 36 (4) ◽  
pp. 491-499 ◽  
Author(s):  
BARBARA MANDULA ◽  
ERNEST BEUTLER
Keyword(s):  
Red Blood Cells ◽  
Glutathione Reductase ◽  
Blood Cells ◽  
Reductase Activity ◽  
Red Cell ◽  
Glucose Medium ◽  
Human Red Blood Cells ◽  
Glutathione Reductase Activity ◽  
Normal Human ◽  
Turn Over

Abstract Intact human red blood cells can synthesize FAD and FMN from riboflavin. The rate of synthesis of FAD is linearly proportional to the concentration of riboflavin in the medium at levels below 0.9 µM. With 0.9 µM riboflavin, the rate of synthesis is about 0.1 mµmole FAD/ml. red blood cells/hour. Incubation of red blood cells with riboflavin can result in increased red cell glutathione reductase activity when the enzyme is measured in the absence of added FAD. This indicates that the FAD concentration in the red cells increased during the incubation. The rate of incorporation of radioactive riboflavin into red blood cells is the same whether the cells are suspended in plasma or in a phosphate-saline-glucose medium. The time it takes for half the FAD in normal human red blood cells to turn over is calculated to be about 6 days, assuming a single mixing pool of red cell FAD.

Sign in / Sign up

Export Citation Format

Share Document