High-throughput screening under demanding conditions: Cu/ZnO catalysts in high pressure methanol synthesis as an example

ABSTRACT We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using UDP-MurNAc-Nε -dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate, and a partially purified preparation of MraY solubilized from membranes of an Escherichia coli overproducing strain. Two versions of the assay were developed, one consisting of the high-pressure liquid chromatography separation of the substrate and product (dansylated lipid I) and the other, without separation and adapted to the high-throughput format, taking advantage of the different fluorescence properties of the nucleotide and lipid I in the reaction medium. The latter assay was validated with a set of natural and synthetic MraY inhibitors.

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Design of Low Cost Pipe Fitting Device for High-throughput Screening Reactor for Screening of Methanol Synthesis Catalyst

Journal of the Japan Petroleum Institute ◽

10.1627/jpi.47.218 ◽

2004 ◽

Vol 47 (3) ◽

pp. 218-221 ◽

Cited By ~ 1

Author(s):

Kohji Omata ◽

Masahiko Hashimoto ◽

Yuhsuke Watanabe ◽

Sutarto ◽

Tetsuo Umegaki ◽

...

Keyword(s):

High Throughput ◽

Methanol Synthesis ◽

High Throughput Screening ◽

Low Cost ◽

Pipe Fitting ◽

Methanol Synthesis Catalyst

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High-throughput Screening of Heterogeneous Catalyst under Pressurized Conditions. Optimization of Cu-Mn Oxide Catalyst for Methanol Synthesis.

Journal of The Japan Petroleum Institute ◽

10.1627/jpi1958.43.317 ◽

2000 ◽

Vol 43 (4) ◽

pp. 317-319 ◽

Cited By ~ 13

Author(s):

Kohji OMATA ◽

Gunji ISHIGURO ◽

Muneyoshi YAMADA

Keyword(s):

Heterogeneous Catalyst ◽

High Throughput ◽

Methanol Synthesis ◽

High Throughput Screening ◽

Oxide Catalyst ◽

Mn Oxide

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Catalyst Development for Methanol Synthesis Using Parallel Reactors for High-throughput Screening Based on a 96 Well Microplate System

Journal of the Japan Petroleum Institute ◽

10.1627/jpi.46.328 ◽

2003 ◽

Vol 46 (5) ◽

pp. 328-334 ◽

Cited By ~ 11

Author(s):

Kohji OMATA ◽

Yuhsuke WATANABE ◽

Tetsuo UMEGAKI ◽

Masahiko HASHIMOTO ◽

Muneyoshi YAMADA

Keyword(s):

High Throughput ◽

Methanol Synthesis ◽

High Throughput Screening ◽

Catalyst Development

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High-throughput counter-diffusion capillary crystallization andin situdiffraction using high-pressure freezing in protein crystallography

Journal of Applied Crystallography ◽

10.1107/s0021889812034061 ◽

2012 ◽

Vol 45 (5) ◽

pp. 999-1008 ◽

Cited By ~ 7

Author(s):

Mareike Kurz ◽

Beat Blattmann ◽

Andres Kaech ◽

Christophe Briand ◽

Paul Reardon ◽

...

Keyword(s):

High Pressure ◽

Low Temperature ◽

Data Collection ◽

High Throughput ◽

Diffraction Data ◽

High Throughput Screening ◽

Structural Information ◽

Major Step ◽

Counter Diffusion

Post-crystallization treatments such as manual fishing of crystals and soaking in cryoprotectant solutions, especially of large macromolecular complexes and membrane proteins, are cumbersome and often lead to crystal damage and reduced diffraction data quality. Here, a capillary crystallization plate is presented that simultaneously allows counter-diffusion crystallization at the nanolitre scale in a high-throughput screening mode, low-temperaturein situdiffraction data collection from crystals after cryoprotection and low-temperaturein situdata collection of crystals without the addition of any cryoprotectant after high-pressure (HP) freezing. The development of this plate and plunge cooling of crystals in the capillaries is a major step towards implementing automatedin situhigh-throughput crystal diffraction data collection at a synchrotron beamline. In combination with HP freezing this offers a new opportunity to obtain structural information from fragile crystals of supramolecular complexes that might otherwise not be feasible.

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