Thin-layer chromatographic detection of glycolaldehyde using a fluorescence reaction with σ-aminodiphenyl

1974 ◽  
Vol 88 (2) ◽  
pp. 356-360 ◽  
Author(s):  
Tadashi Nakai ◽  
Takafumi Ohta ◽  
Noriko Wanaka ◽  
Daiji Beppu
Keyword(s):  
Thin Layer ◽  
Fluorescence Reaction ◽  
Chromatographic Detection

Use of diphenylamine reagent for high-performance thin-layer chromatographic detection of organochloro insecticide endosulfan in biological samples

2019 ◽  
Vol 32 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Umakant D. Pawar ◽  
Chandrakant D. Pawar ◽  
Ulka K. Kulkarni ◽  
Rajendra K. Pardeshi ◽  
Mazahar Farooqui ◽  
...  
Keyword(s):  
Thin Layer ◽  
Biological Samples ◽  
High Performance ◽  
Chromatographic Detection

Improved Ammonium Molybdate Method for Thin Layer Chromatographic Detection of Organophosphate Residues

1980 ◽  
Vol 63 (4) ◽  
pp. 756-757
Author(s):  
A Satyanarayana Murty ◽  
B Roosevelt Rajabhushanam ◽  
K Christopher ◽  
A Venkata Ramani
Keyword(s):  
Ascorbic Acid ◽  
Thin Layer ◽  
Inorganic Phosphate ◽  
Ammonium Molybdate ◽  
Tlc Plates ◽  
Chromatographic Detection ◽  
Molybdate Method

Abstract An improved ammonium molybdate method for detecting organophosphate residues on thin layer chromatographic (TLC) plates is described. After development, the plates are heated at 110°C for 2 h and the resultant inorganic phosphate is reacted with ammonium molybdate to form phosphomolybdate which is then reduced to a blue complex by ascorbic acid. Quantities as low as 0.1–0.2 μg residues could be detected on TLC plates by this method.

Sensitive Thin Layer Chromatographic Detection of High Fructose Corn Sirup and Other Adulterants in Honey

1979 ◽  
Vol 62 (4) ◽  
pp. 917-920 ◽  
Author(s):  
Irene Kushnir
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Plant Source ◽  
High Fructose ◽  
Highly Sensitive ◽  
Rf Values ◽  
Chromatographic Detection ◽  
Total Mixture ◽  
Layer Chromatography

Abstract A highly sensitive procedure has been developed to detect the undeclared addition of high fructose corn sirup (HFCS) to honey. Carbohydrates must be separated first to achieve the requisite degree of sensitivity: charcoal-Celite chromatography was used to isolate a fraction containing oligo- and polysaccharides. The fraction was then concentrated and examined by thin layer chromatography on silica gel. Pure honeys yielded only 1 or 2 blue-grey or bluebrown spots at Rf values >0.35; a series of spots or blue streaks extending from the origin characterized adulterated samples. The method detects HFCS and conventional honey adulterants at levels as low as 10% or less of the total mixture. In addition, the procedure detects the presence in honey of all starch-derived sugar sirups tested thus far, regardless of the plant source.

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