SCREENING OF EXTRACTION METHOD FOR ALKALOID ENRICHMENT OF PEPEROMIA PELLUCIDA (L.) KUNTH

Objective: The aims of this study were to investigate the effect of extraction method modification of Peperomia pellucida (L.) Kunth herbs for alkaloid enrichment and thin layer chromatography (TLC) profile.Methods: The dried herbs were extracted with six different methods (including; method A, B, C, D, and E). The obtained extract solution was evaporated and was analyzed. Alkaloid content identified using Dragendorff spray reagent. The gravimetric method was used to determine of total alkaloid, and TLC profile was analyzed using a densitometer at a wavelength of 254 nm and 366 nm.Results: The results showed a difference from all modification of extraction method for alkaloid enrichment and their TLC profile. The use of eluent dichloromethane (DCM):methanol (95:5) was potential for isolation of the compound. From whole extraction methods, only one that tested positive for alkaloids was method A. Total alkaloids in DCM fraction amounted to 29.59 mg/g piperine.Conclusion: Based on the above results, method A can be applied for isolation of alkaloid compound from this herb.

Phytochemical Screening and TLC Profile of Fruits and Flowers of Alstonia venenata R. Br.

Alstonia venenata R. Br. belonging to the family Apocynaceae is a tall evergreen shrub distributed throughout Peninsular India. Stem-bark, root-bark, fruits and leaves are used by many tribal communities and also in Ayurveda. The study investigates the phytochemical composition of hexane, butanol, methanol and water extracts of Alstonia venenata fruits and flowers as well as the TLC profile of hexane extracts of fruits and flowers. Quantitative data of the wet and dry weight, yields from different solvent fractions and percentage yields were noted. The phytochemical analysis revealed the presence of secondary metabolites such as alkaloids, steroids, terpenoids, saponins, flavonoids, tannins and phenolic compounds from the various extracts. Alkaloids were present in all the fractions tested. Methanol extracts of fruits and flowers showed the presence of major phytoconstituents. TLC profile of hexane extracts of fruits and flowers were developed using anisaldehyde sulphuric acid/ceric sulphate (steroids/terpenoids) and Dragendorff’s spray reagents (alkaloids). Petroleum ether: Chloroform: Methanol (5: 4.5: 0.5) showed good resolution for the hexane extracts of fruit and flower when treated with Dragendorff’s spray reagent. Petroleum ether: Chloroform (1:1) was best for the hexane exacts of flowers and fruits when sprayed with ceric sulphate spray reagent