High-speed liquid and thin-layer chromatography of polychlorinated biphenyls

1976 ◽  
Vol 128 (1) ◽  
pp. 101-110 ◽  
Author(s):  
U.A.Th. Brinkman ◽  
A. de Kok ◽  
G. de Vries ◽  
H.G.M. Reymer
Keyword(s):  
Polychlorinated Biphenyls ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Speed ◽  
Layer Chromatography

Semiquantitative Determination of Polychlorinated Biphenyls in Tissue Samples by Thin Layer Chromatography

1971 ◽  
Vol 54 (3) ◽  
pp. 548-550
Author(s):  
Bernard M Mulhern ◽  
Eugene Cromartie ◽  
William L Reichel ◽  
Andre A Belisle
Keyword(s):  
Polychlorinated Biphenyls ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Polychlorinated Biphenyl ◽  
Chlorinated Pesticides ◽  
Tissue Samples ◽  
Florisil Column ◽  
Florisil Column Chromatography ◽  
Layer Chromatography

Abstract A method is described for the analysis of polychlorinated biphenyl (PCB) compounds in tissue samples. Cleanup by hexane-acetonitrile partitioning and Florisil column chromatography are performed on samples before oxidative treatment to convert DDE to DCBP. PCB components are then determined semi-quantitatively by TLC. No prior separation of PCB from chlorinated pesticides is required. The lower limit of sensitivity is 0.2 μg.

Thin Layer Chromatographic Separation of Two Chlorodibenzo-p-Dioxins from Some Polychlorinated Biphenyls and Organochlorine Pesticides

1971 ◽  
Vol 54 (6) ◽  
pp. 1429-1431
Author(s):  
D T Williams ◽  
B J Blanchfield
Keyword(s):  
Gas Chromatography ◽  
Polychlorinated Biphenyls ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Electron Capture ◽  
Chromatographic Separation ◽  
Corn Oil ◽  
Chromatographic Procedure ◽  
Layer Chromatography

Abstract A thin layer chromatographic procedure is described for isolating 2,3,7,8-tetrachlorodibenzo-p-dioxin and octachlorodibenzo-p-dioxin. This method separates these compounds from polychlorobiphenyls and organochlorine pesticides. After isolation by thin layer chromatography, the chlorodibenzo-p-dioxins are analyzed by using electron capture gas chromatography. The application of the method to corn oil is discussed.

Application of High-Speed Liquid Chromatography to the Analysis of Aflatoxins

1973 ◽  
Vol 56 (4) ◽  
pp. 827-830 ◽  
Author(s):  
James N Seiber ◽  
Dennis P H Hsieh
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Porous Layer ◽  
High Speed ◽  
Aspergillus Parasiticus ◽  
Coefficients Of Variation ◽  
Silica Adsorbent ◽  
Layer Chromatography

Abstract Partial resolution of aflatoxins B1, B2, G1, G2, and P1 was achieved by high-speed liquid chromatography (HSLC) on a porous layer silica adsorbent, using chloroform-isooctane as the eluting solvent and a 254 nm UV monitor for detection. The resolution was somewhat less than, although comparable with, that obtained by thin layer chromatography, using Adsorbosil-1 adsorbent and fluorodensitometric detection. The HSLC response to B1 and G1 was linear in the 400–3000 ng range, allowing application of the technique to the quantitative analysis of B1 and G1 in crude extracts of Aspergillus parasiticus cultures. The coefficients of variation (precision) were 4.2% for B1 and 23.2% for G1 in a series of 4 replicate injections. The advantages and limitations of the technique for quantitative analysis and isolation are compared with those of more conventional chromatographic methods.

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