Thin-layer chromatographic separation of free fatty acids

1975 ◽  
Vol 114 (1) ◽  
pp. 274-279 ◽  
Author(s):  
J.F. Rodrigues de Miranda ◽  
T.D. Eikelboom
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Chromatographic Separation

Diacylglycerol Biosynthesis in Everted Sacs of Rat Intestinal Mucosa

1975 ◽  
Vol 53 (11) ◽  
pp. 1170-1183 ◽  
Author(s):  
W. C. Breckenridge ◽  
A. Kuksis
Keyword(s):  
Fatty Acids ◽  
Phosphatidic Acid ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Thin Layer Chromatography ◽  
Intestinal Mucosa ◽  
Gas Liquid Chromatography ◽  
Acid Pathway ◽  
Layer Chromatography ◽  
Everted Sacs

The molecular specificity in the biosynthesis of diacylglycerols by rat intestinal mucosa was examined by means of radioactive markers, thin-layer chromatography with silver nitrate and gas-liquid chromatography with radioactivity monitoring. Bile salt micelles of alternately labeled monoacylglycerols and free fatty acids were incubated with everted sacs of intestinal mucosa for various periods of time and the diacylglycerols were isolated by solvent extraction and thin-layer chromatography. Stereospecific analyses of the X-1,2-diacylglycerols labeled from 2-monoacylgiycerols showed that the sn-1,2-isomers (45–55%) were slightly in excess of the sn-2,3-isomers (34–45%) with the X-1,3-diacylglycerols accounting for the rest of the radioactivity (5–10%). This suggests that racemic diacylglycerols may be intermediates in the resynthesis of dietary fat in rat intestinal mucosa. Detailed analyses of the molecular species of the sn-1,2-diacylglycerols labeled from free fatty acids revealed that 10–45% of the total did not contain the acid present in the 2-monoacylglycerol supplied, and therefore had originated from the phosphatidic acid pathway. These findings are at variance with those obtained in isolated microsomes, which have suggested an inhibition of the phosphatidic acid pathway by monoacylglycerols as well as have given evidence of an exclusive synthesis of sn-1,2-diacylglycerols from 2-monoacylglycerols.

An improved rapid thin-layer chromatographic—gas—liquid chromatographic procedure for the determination of free fatty acids in plasma

1989 ◽  
Vol 183 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Hendrik Y. Tichelaar ◽  
A.J.Spinnler Benadé ◽  
Annette K. Daubitzer ◽  
Theunis J.v.W. Kotze
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

scholarly journals Effect of histamine on lipid composition of rat bile

2014 ◽  
Vol 68 (3) ◽  
pp. 93-95
Author(s):  
S. Athamnah ◽  
V. Baranovsky ◽  
E. Reshetnik ◽  
S. Veselsky ◽  
P. Yanchuk
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Sodium Thiopental ◽  
Physical And Chemical ◽  
Layer Chromatography ◽  
Rat Bile ◽  
And Chemical Properties

To investigate the effect of histamine (8 mkg/kg, i/v) on the concentration of lipids in the rats bile. Using the method of thin layer chromatography concentrations of phospholipids, cholesterol and its esters, free fatty acids, triglycerides were determined in the rats bile collected in acute experiments (anesthesia, sodium thiopental, 50 mg/kg). The results were calculated using Statistica 7.0; p<0,05. Histamine increased concentration of phospholipids, cholesterol, its esters and reduced content of free fatty acids and triglycerides in the rats bile. Probably, histamine, acting on transport lipid components in bile effect on its physical and chemical properties and the maintenance of cholesterol in mixed bile micelles. Histamine affects the metabolic conversion and transport of lipids in the liver cells, causing the increase in the concentration of phospholipids, cholesterol and its esters in bile and decrease it in the content of free fatty acids and triglycerides.

Simplified Measurement of Monoglycerides, Diglycerides, Triglycerides, and Free Fatty Acids in Biological Samples

1971 ◽  
Vol 17 (3) ◽  
pp. 145-147 ◽  
Author(s):  
A Marzo ◽  
P Ghirardi ◽  
D Sardini ◽  
G Meroni
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Biological Samples ◽  
Lipid Classes

Abstract A sensitive (5 to 100 µg) and reproducible (±3.54% SD) method is described for determining monoglycerides, diglycerides, triglycerides, and free fatty acids. After these lipid classes are separated from extracts of plasma or tissues by a single thin-layer chromatographic development, they are eluted into concentrated H2SO4 at 200°C. The resulting color is stable for more than six days.

Improved staining characteristics of serum lipids after halogenation and esterification of thin-layer chromatograms.

1977 ◽  
Vol 23 (5) ◽  
pp. 835-841 ◽  
Author(s):  
W R Nelson ◽  
S Natelson
Keyword(s):  
Fatty Acids ◽  
Thin Layer ◽  
Free Fatty Acids ◽  
Free Cholesterol ◽  
Relative Concentration ◽  
Guanidine Hydrochloride ◽  
Internal Standard ◽  
Double Bonds ◽  
Erythrosine B ◽  
Iodine Monobromide

Abstract We describe a procedure for treatment of thin-layer chromatographic serum lipid patterns so that they may be stained by dyes for evaluation by densitometry. After development of the chromatogram [Clin. Chem. 18, 384 (1972)] the plates are dried and sprayed with butyryl chloride. This esterifies the free cholesterol. After drying, the plate is treated with iodine monobromide, to add iodline to the double bonds. The triacylglycerols (triglycerides), cholesterol esters, free cholesterol, and phospholipids are now all in the form of esters with no unsaturated double bonds. The free fatty acids are also now saturated. The chromatogram is now stained with basic fuchsine in acetate buffer (0.1 mol/liter, pH 5.0). Excess stain is removed with a buffered solution of guanidine hydrochloride. Erythrosine B may also be used. With basic fuchsine the background will be a uniform pink. With erythrosine B the background is white, but the stain tends to be washed out of the free fatty acids. The chromatograms are evaluated by densitometry, with use of a 540-nm filter for basic fuchsine and a 520-nm filter for erythrosine B. The stained chromatograms and densitometric scans accurately represent the relative concentration of the various lipid fractions as compared to that of an internal standard, and correlate with the nature of the disease being explored.

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