On-line microbore high-performance liquid chromatography—capillary gas chromatography—mass spectrometry

1987 ◽  
Vol 409 ◽  
pp. 205-212 ◽  
Author(s):  
Thomas V. Raglione ◽  
Jo Anna Troskosky ◽  
Richard A. Hartwick
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Capillary Gas Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
On Line

Identification and measurement of 4-oestren-3,17-dione (19-norandrostenedione) in porcine ovarian follicular fluid using high performance liquid chromatography and capillary gas chromatography–mass spectrometry

1985 ◽  
Vol 107 (3) ◽  
pp. 375-381 ◽  
Author(s):  
M. W. Khalil ◽  
J. S. Walton
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Follicular Fluid ◽  
Capillary Gas Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ovarian Follicular Fluid

ABSTRACT 4-Oestren-3,17-dione (19-norandrostenedione) has been identified as a major steroid in porcine ovarian follicular fluid for the first time. This steroid was isolated by C18 reverse-phase high performance liquid chromatography (HPLC) and its structure was established unambiguously by capillary gas chromatography–mass spectrometry. The mean concentration in pooled fluid obtained from prepubertal gilts determined by HPLC and capillary gas chromatography was 1·71 μmol/l. The level of this steroid in fluid obtained from large preovulatory follicles of sows exceeded 21·0 μmol/l on days 19 and 20 of the oestrous cycle. The high levels of this steroid within the follicle during the follicular phase might imply a possible biological role in follicular development and oocyte maturation. J. Endocr. (1985) 107, 375–381

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