Purification of membrane protein complexes isolated from a cyanobacterial thylakoid membrane by high-performance liquid chromatography

1990 ◽  
Vol 512 ◽  
pp. 219-226 ◽  
Author(s):  
Matthias Rögner
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Membrane Protein ◽  
Thylakoid Membrane ◽  
High Performance ◽  
Protein Complexes ◽  
Membrane Protein Complexes

scholarly journals Regulatory factors for the assembly of thylakoid membrane protein complexes

2012 ◽  
Vol 367 (1608) ◽  
pp. 3420-3429 ◽  
Author(s):  
Wei Chi ◽  
Jinfang Ma ◽  
Lixin Zhang
Keyword(s):  
Membrane Protein ◽  
Thylakoid Membrane ◽  
Molecular Mechanisms ◽  
Current Knowledge ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Regulatory Factors ◽  
Photosynthetic Organisms ◽  
Photosynthetic Complexes ◽  
Membrane Protein Complexes

Major multi-protein photosynthetic complexes, located in thylakoid membranes, are responsible for the capture of light and its conversion into chemical energy in oxygenic photosynthetic organisms. Although the structures and functions of these photosynthetic complexes have been explored, the molecular mechanisms underlying their assembly remain elusive. In this review, we summarize current knowledge of the regulatory components involved in the assembly of thylakoid membrane protein complexes in photosynthetic organisms. Many of the known regulatory factors are conserved between prokaryotes and eukaryotes, whereas others appear to be newly evolved or to have expanded predominantly in eukaryotes. Their specific features and fundamental differences in cyanobacteria, green algae and land plants are discussed.

Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes

2011 ◽  
Vol 439 (2) ◽  
pp. 207-214 ◽  
Author(s):  
Sari Järvi ◽  
Marjaana Suorsa ◽  
Virpi Paakkarinen ◽  
Eva-Mari Aro
Keyword(s):  
Membrane Protein ◽  
Thylakoid Membrane ◽  
Protein Complexes ◽  
Higher Plant ◽  
Native Page ◽  
Light Harvesting Complexes ◽  
Thylakoid Protein ◽  
Electrophoresis Method ◽  
Membrane Protein Complexes ◽  
Photosynthetic Machinery

Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owing to a great variety of native-PAGE and thylakoid-solubilization systems. In the present paper, we describe optimized methods for separation of higher plant thylakoid membrane protein complexes by native-PAGE addressing particularly: (i) the use of detergent; (ii) the use of solubilization buffer; and (iii) the gel electrophoresis method. Special attention is paid to separation of high-molecular-mass thylakoid membrane super- and mega-complexes from Arabidopsis thaliana leaves. Several novel super- and mega-complexes including PS (photosystem) I, PSII and LHCs (light-harvesting complexes) in various combinations are reported.

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