Separation of ganglioside molecular species, with homogeneous long-chain base composition, by reversed-phase thin-layer chromatography

1984 ◽  
Vol 315 ◽  
pp. 395-400 ◽  
Author(s):  
Giuliano Gazzotti ◽  
Sandro Sonnino ◽  
Riccardo Ghidoni
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Molecular Species ◽  
Base Composition ◽  
Reversed Phase ◽  
Chain Base ◽  
Long Chain Base ◽  
Layer Chromatography ◽  
Long Chain

Glycosphingolipids in Secondary Lysosomes Prepared from Rat Liver

1974 ◽  
Vol 52 (6) ◽  
pp. 507-513 ◽  
Author(s):  
S. Huterer ◽  
J. R. Wherrett
Keyword(s):  
Gas Chromatography ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Long Chain Base ◽  
Triton Wr 1339 ◽  
Secondary Lysosomes ◽  
Layer Chromatography ◽  
Long Chain

Glycosphingolipid classes were isolated from unfractionated liver and from the buoyant fraction of secondary lysosomes following pretreatment of rats with Triton WR-1339. They were identified by thin-layer chromatography and by sugar analysis using gas chromatography and were quantified by estimating long chain base. Hematoside containing N-acetyl neuraminic acid, glucosyl, lactosyl, trihexosyl, and galactosamine-containing tetrahexosyl ceramides were identified in unfractionated liver in the following concentrations, respectively: 0.89, 0.59, 0.19, 0.06, and 0.1 nmol/mg protein. The same glycosphingolipid classes were identified in buoyant Triton-filled lysosomes where their respective concentrations in terms of protein were 8.6, 21, 28, 34, and 28 times as great as in unfractionated liver. The lysosome fraction was enriched 28-fold in acid phosphatase. These results demonstrate that some glycosphingolipid classes are concentrated in secondary lysosomes to a degree expected of markers for primary lysosomes and plasma membrane. Secondary lysosomes are thought to be derived from these latter two structures.

Reversed-phase thin-layer chromatography of some foodstuff dyes

2003 ◽  
Vol 16 (4) ◽  
pp. 276-279 ◽  
Author(s):  
Dušanka Milojković-Opsenica ◽  
Kristina Lazarević ◽  
Vojkan Ivačković ◽  
Živoslav Tešić
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Layer Chromatography

Chromo-ionophoric Properties of para-(5-phthalhydrazide-azo)-phenylene-N-aza-15-crown-5 in the Presence of Lithium and Sodium Ions

2008 ◽  
Vol 59 (10) ◽  
Author(s):  
Rodica Daniela Baratoiu ◽  
Radu Socoteanu ◽  
Radu Cristian Mutihac ◽  
Anca Elena Barbu ◽  
Adrian Beteringhe ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Complex Stability ◽  
Sodium Ions ◽  
Layer Chromatography

Investigations on the hydrophobic and ionophoric character of para-(5-phthalhydrazide-azo)-phenylene-N-aza-15-crown- (1) were carried out by reversed phase thin layer chromatography (RP-TLC) and spectrophotometric measurements. The study included complexes with Li+ and Na+ cations (as perchlorates in acetonitrile and as dibenzyldithiocarbamate salts in chloroform). In both cases, the stoichiometry of complexes was 1:1:1 (1:M+:anion pair). The complex stability (logKS ) is similar.

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