Systematic search for suitable two-phase solvent systems for high-speed counter-current chromatography

1991 ◽  
Vol 538 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Fumie Oka ◽  
Hisao Oka ◽  
Yoichiro Ito
Keyword(s):  
High Speed ◽  
Systematic Search ◽  
Two Phase ◽  
Solvent Systems ◽  
Counter Current

High-Purity Isolation of Trans-Resveratrol from Rhizma Polygoni Cuspidati by High-Speed Counter-Current Chromatography

2013 ◽  
Vol 690-693 ◽  
pp. 1335-1339
Author(s):  
Jian Chao Deng ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Su Ping Lu ◽  
Yan Yan Wu ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Separation And Purification ◽  
Two Phase ◽  
Dry Extract ◽  
H Nmr ◽  
Assisted Extraction ◽  
Solvent Systems ◽  
Counter Current ◽  
Chloroform Methanol

In this paper, high-speed counter-current chromatography (HSCCC) was established and successfully applied for the separation and purification of trans-resveratrol from Rhizma Polygoni Cuspidati. After extracted by microwave assisted extraction (MAE), the dry extract was directly dissolved in HSCCC solvent and introduced to HSCCC separation system. Two-phase solvent system of HSCCC was optimized. Trans-resveratrol was separated from extract of Rhizma Polygoni Cuspidati by high-speed counter-current chromatography in two-step using two different solvent systems composed of chloroform-methanol-water (4:3:2, v/v/v) and n-hexane-ethyl acetate-methanol-water (3:5:4:6, v/v/v/v). From 200 mg of crude extract, 2.5 mg of trans-resveratrol was obtained at over 99% purity by HPLC analyses, and its chemical structure was identified by ESI-MS, 1H NMR.

Modelling and optimization of a two phase system for the separation of equol enantiomers by recycling high-speed counter-current chromatography

2015 ◽  
Vol 26 (15-16) ◽  
pp. 821-828 ◽  
Author(s):  
Kewen Tang ◽  
Genlin Sun ◽  
Panliang Zhang ◽  
Weijun Yang ◽  
Congshan Zhou ◽  
...  
Keyword(s):  
High Speed ◽  
Phase System ◽  
Two Phase ◽  
Counter Current ◽  
Modelling And Optimization

Three-phase solvent systems for comprehensive separation of a wide variety of compounds by high-speed counter-current chromatography

2006 ◽  
Vol 1133 (1-2) ◽  
pp. 119-125 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Akio Yanagida ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
High Speed ◽  
Three Phase ◽  
Solvent Systems ◽  
Counter Current

scholarly journals Purification of Flavonoids from Mulberry Leaves via High-Speed Counter-Current Chromatography

Processes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 91 ◽  
Author(s):  
Pian Zhang ◽  
Kang-Ling Zhu ◽  
Jun Zhang ◽  
Yan Li ◽  
Heng Zhang ◽  
...  
Keyword(s):  
Flow Rate ◽  
Mobile Phase ◽  
High Speed ◽  
Separation Efficiency ◽  
Phase Flow ◽  
Mobile Phase Flow Rate ◽  
Mulberry Leaves ◽  
Mulberry Leaf ◽  
Solvent Systems ◽  
Counter Current

In order to obtain high-purity flavonoid products, the extracts from mulberry leaves were separated and purified via high-speed counter-current chromatography (HSCCC). Moreover, the product was detected via high-performance liquid chromatography (HPLC). The characteristic absorption wavelength of the rutin standard for HSCCC detection and HPLC analysis at 257 nm was tested by ultraviolet scanning analysis. The effect of solvent systems and mobile phase flow rate on the separation efficiency were then researched. Finally, the solvent system of V(ethyl acetate):V(n-butanol):V(water) = 4:1:5 was selected as the operating system for HSCCC. This work theoretically analyzed the impact of the molecular structure and polarity of flavonoids on the choice of solvent systems. The results showed that the mobile phase flow rate had a great influence on the separation efficiency. Furthermore, the separation efficiency increased as the mobile phase flow rate decreased. When the mobile phase flow rate was 5 mL/min, the peak time for flavonoids was 140 min, the retention of the stationary phase was 56.4%, and the purity of the product reached 93.8%. The results of this study greatly improved the purity of flavonoids in mulberry leaf and provided a strong support for the separation and purification of mulberry leaf extract.

Isolation and Purification of Five Phloroglucinol Derivatives From Agrimonia Pilosa Ledeb by Counter-Current Chromatography Using Non-Aqueous Solvent Systems

2020 ◽  
Author(s):  
Lulu Fu ◽  
Chenghua Lu ◽  
Shengqiang Tong ◽  
Min Xu ◽  
Lan Tang ◽  
...  
Keyword(s):  
Closed Loop ◽  
Solvent System ◽  
The Other ◽  
Second Step ◽  
Two Phase ◽  
Isolation And Purification ◽  
Aqueous Solvent ◽  
Phloroglucinol Derivatives ◽  
Solvent Systems ◽  
Counter Current

Abstract Five non-polar phloroglucinol derivatives, viz. pseudo-aspidin, α-kosin and agripinol A-C were isolated and purified from Agrimonia pilosa Ledeb by semi-preparative counter-current chromatography. The separation was performed by a two-step elution with non-aqueous solvent systems. In the first step, an elution mode of a two-phase solvent system consisting of n-hexane-acetonitrile-dichloromethane-methanol (6:6:0.5:0.5, v/v/v/v) was used. We obtained sample Ι containing three components (47.0 mg) and sample ΙΙ containing two components (24.8 mg) from crude extract (371.0 mg). In the second step, sample Ι was successfully separated by closed-loop recycling mode with a solvent system consisting of n-hexane-acetonitrile-dichloromethane (10:7:3, v/v/v), yielding 17.8 mg of pseudo-aspidin, 18.5 mg of α-kosin and 6.4 mg of agripinol A. The other two compounds—8.7 mg of agripinol B and 13.6 mg of agripinol C—were obtained from sample ΙΙ in the same manner. All the isolated compounds had a high purity exceeding 95%.

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