Simultaneous determination of stevioside, rebaudioside A and C and dulcoside A in foods by high-performance liquid chromatography

1989 ◽  
Vol 474 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Yoshimi Kitada ◽  
Michiko Sasaki ◽  
Yutaka Yamazoe ◽  
Hiroyuki Nakazawa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Rebaudioside A

scholarly journals Simultaneous determination of steviol glycosides in stevia by high performance liquid chromatography

2019 ◽  
Vol 2 (4) ◽  
pp. 2-8
Author(s):  
Huyen Trang Luu Thi ◽  
Thanh Huong Doan Thi ◽  
Trang Vu Thi ◽  
Son Tran Cao ◽  
Anh Huong Nguyen Thi ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Calibration Curves ◽  
Oasis Hlb

A method for determination of steviol glucosides in stevia using high performance liquid chromatography was developed and validated. The compounds were extracted from the matrices with methanol for 60 minutes, cleaned by Oasis HLB column and then determined by HPLC using C18 column (250 mm x 4.6 mm x 5 µm) and PDA detector. The calibration curves were linear in range of 1 to 100 μg/mL; the RSD was of 1.39 ­ 2.85%; and the recovery was of 93 ­ 105%. The method was applied to analyze 09 stevia samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder). The results showed that composition of steviol glycosides differs from sample to sample. Besides, stevioside and rebaudioside A are the most abundant steviol glycosides in the samples.

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