scholarly journals Simultaneous determination of steviol glycosides in stevia by high performance liquid chromatography

2019 ◽  
Vol 2 (4) ◽  
pp. 2-8
Author(s):  
Huyen Trang Luu Thi ◽  
Thanh Huong Doan Thi ◽  
Trang Vu Thi ◽  
Son Tran Cao ◽  
Anh Huong Nguyen Thi ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Calibration Curves ◽  
Oasis Hlb

A method for determination of steviol glucosides in stevia using high performance liquid chromatography was developed and validated. The compounds were extracted from the matrices with methanol for 60 minutes, cleaned by Oasis HLB column and then determined by HPLC using C18 column (250 mm x 4.6 mm x 5 µm) and PDA detector. The calibration curves were linear in range of 1 to 100 μg/mL; the RSD was of 1.39 ­ 2.85%; and the recovery was of 93 ­ 105%. The method was applied to analyze 09 stevia samples collected from markets in Hanoi (including dry stevia rebaudiana, stevia powder). The results showed that composition of steviol glycosides differs from sample to sample. Besides, stevioside and rebaudioside A are the most abundant steviol glycosides in the samples.

scholarly journals High-performance liquid chromatography and derivative spectrophotometry for simultaneous determination of pravastatin and fenofibrate in the dosage form

2014 ◽  
Vol 64 (4) ◽  
pp. 433-446 ◽  
Author(s):  
Mohamed M. Hefnawy ◽  
Mostafa S. Mohamed ◽  
Mohammed A. Abounassif ◽  
Amer M. Alanazi ◽  
Gamal A. E. Mostafa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Particle Diameter ◽  
Second Order ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Calibration Curves

Abstract High performance liquid chromatography (HPLC) and second-order derivative spectrophotometry have been used for simultaneous determination of pravastatin (PS) and fenofibrate (FF) in pharmaceutical formulations. HPLC separation was performed on a phenyl HYPERSIL C18 column (125 mm × 4.6 mm i.d., 5 μm particle diameter) in the isocratic mode using a mobile phase acetonitrile/0.1 % diethyl amine (50:50, V/V, pH 4.5) pumped at a flow rate of 1.0 mL min-1. Measurement was made at 240 nm. Both drugs were well resolved on the stationary phase, with retention times of 2.15 and 5.79 min for PS and FF, respectively. Calibration curves were linear (R = 0.999 for PS and 0.996 for FF) in the concentration range of 5-50 and 20-200 µg mL-1 for PS and FF, respectively. Pravastatin and fenofibrate were quantitated in combined preparations also using the second-order derivative response at 237.6 and 295.1 nm for PS and FF, respectively. Calibration curves were linear, with the correlation coefficient R = 0.999 for pravastatin and fenofibrate, in the concentration range of 5-20 and 3-20 µg mL-1 for PS and FF, respectively. Both methods were fully validated and compared, the results confirmed that they were highly suitable for their intended purpose.

Sign in / Sign up

Export Citation Format

Share Document