Quantitative determination of α- and γ-2,4-dinitrophenyl-isomers of α,γ-diaminobutyric acid with an automatic amino acid analyzer as a method of studying Nα ⇄ Nγ migration in peptides of α,γ-diaminobutyric acid and polymyxin M

1966 ◽  
Vol 24 ◽  
pp. 61-67 ◽  
Author(s):  
A.B. Silaev ◽  
L.A. Baratova ◽  
G.S. Katrukha
Keyword(s):  
Amino Acid ◽  
Quantitative Determination ◽  
Automatic Amino Acid Analyzer ◽  
Diaminobutyric Acid ◽  
Amino Acid Analyzer

Automated chromatographic analysis of free monosubstituted guanidines in physiological fluids

1969 ◽  
Vol 47 (6) ◽  
pp. 657-664 ◽  
Author(s):  
D. J. Durzan
Keyword(s):  
Amino Acid ◽  
Quantitative Determination ◽  
Special Reference ◽  
Analytical Method ◽  
Chromatographic Analysis ◽  
Spruce Budworm ◽  
Guanidine Derivative ◽  
Automated Method ◽  
Amino Acid Analyzer

An automated analytical method for the quantitative determination of monosubstituted guanidines in the physiological fluids of plants and insects is described with special reference to conifers and spruce budworm larvae. Over 25 monosubstituted guanidines of known structure can be separated on columns of an amino acid analyzer of the Spackman, Stein, and Moore type, and determined by the Sakaguchi reagent as modified by Satake and Luck. The automated method minimizes the problem of color decay of the reaction product of the guanidine derivative with the Sakaguchi reagent and reveals the occurrence of several yet unidentified guanidines.

DETERMINATION OF ASPARAGINE, GLUTAMINE AND CITRULLINE IN AQUEOUS SOIL EXTRACTS WITH AN AUTOMATIC AMINO ACID ANALYZER

1968 ◽  
Vol 48 (3) ◽  
pp. 349-354 ◽  
Author(s):  
F. J. Sowden ◽  
K. C. Ivarson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
The Other ◽  
Automatic Amino Acid Analyzer ◽  
Soil Extracts ◽  
Amide Hydrolysis ◽  
Amino Acid Analyzer ◽  
Lithium Citrate ◽  
The Common

A system of separating asparagine, glutamine and citrulline from each other and from the other common amino acids, using lithium buffers and the Technicon amino acid analyzer and C2 resin in a 0.63 × 75-cm column, is described. The column was operated with a buffer flow rate of 37 ml/hour at 30 °C for the first 70 min, then at 50 °C. The buffer was 0.067 M in lithium citrate and adjusted to a pH of 2.80; 2% isopropanol was added to improve the separation of threonine and serine. The analysis was complete through citrulline in 4 hours. If a second buffer, pH 4.15, 0.25 N in lithium was added after 3.5 hours, most of the common acidic and neutral amino acids found in soil extracts were separated in 6 hours. Some data on the determination of asparagine and glutamine by amide hydrolysis is included.

scholarly journals Determination of Methylated Basic Amino Acids with the Amino Acid Analyzer

1973 ◽  
Vol 248 (7) ◽  
pp. 2387-2391 ◽  
Author(s):  
Gladys E. Deibler ◽  
Russell E. Martenson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Basic Amino Acids ◽  
Amino Acid Analyzer

scholarly journals Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

2013 ◽  
Vol 25 (6) ◽  
pp. 3525-3526 ◽  
Author(s):  
Pingmei Yan ◽  
Qing Wang ◽  
Xiaoyan Yan ◽  
Xiao-Hui Chang ◽  
Jian-Zhong Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetically Modified ◽  
Vitamin B2 ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

Fluorometric method for phenylalanine microplate assay adapted for phenylketonuria screening.

1989 ◽  
Vol 35 (10) ◽  
pp. 2112-2115 ◽  
Author(s):  
N S Gerasimova ◽  
I V Steklova ◽  
T Tuuminen
Keyword(s):  
Amino Acid ◽  
Human Plasma ◽  
High Throughput ◽  
Microplate Assay ◽  
Fluorometric Method ◽  
Amino Acid Analyzer ◽  
Screening Sensitivity ◽  
Blood Spot ◽  
Plasma Assay

Abstract We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of phenylalanine. When measurements are performed in an automated reader, the microplate assay has considerable advantages over conventional measurements in cuvettes: smaller volumes of reagents and automation enabling high throughput and convenience. Because the described method is a quantitative one, we can postulate that, compared with the semiquantitative Guthrie inhibition bioassay, this microplate assay is more reliable, easier to perform, and about twofold less costly.

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