Molecular mass characterization of polymers with strongly interacting groups using gel permeation chromatography–light scattering detection

2002 ◽  
Vol 976 (1-2) ◽  
pp. 155-164 ◽  
Author(s):  
I.V Blagodatskikh ◽  
M.V Sutkevich ◽  
N.L Sitnikova ◽  
N.A Churochkina ◽  
T.A Pryakhina ◽  
...  
Keyword(s):  
Light Scattering ◽  
Molecular Mass ◽  
Gel Permeation Chromatography ◽  
Light Scattering Detection ◽  
Strongly Interacting ◽  
Gel Permeation

On the dimensions of intramolecularly crosslinked polymer molecules - III. The measurement of the dimensions of intramolecularly crosslinked polystyrene

1973 ◽  
Vol 334 (1599) ◽  
pp. 477-491 ◽  
Keyword(s):  
Light Scattering ◽  
Molecular Mass ◽  
Mass Distribution ◽  
Molecular Mass Distribution ◽  
Gel Permeation Chromatography ◽  
Polymer Molecules ◽  
Crosslinked Polymer ◽  
Crosslinked Polystyrene ◽  
Gel Permeation ◽  
Molecular Masses

The intramolecularly crosslinked polystyrene molecules, prepared as in part I of this series, were examined by the techniques of light scattering, osmometry, viscometry, and gel permeation chromatography. Their molecular masses and molecular mass distribution remained constant over all the stages of the reaction radii of gyration were determined and the results compared with those predicted in part II of this series.

Purification and Preliminary Characterization of a Thermostable Lactase from Bacillus coagulans T242

2013 ◽  
Vol 690-693 ◽  
pp. 1362-1365
Author(s):  
Shu Juan Jiang ◽  
Guang Qing Mu ◽  
Tao Liu ◽  
Fang Qian
Keyword(s):  
Optimum Condition ◽  
Molecular Mass ◽  
Bacillus Coagulans ◽  
Gel Permeation Chromatography ◽  
Lactase Activity ◽  
High Concentration ◽  
Preliminary Characterization ◽  
Sds Page ◽  
Gel Permeation

A thermostable lactase from Bacillus coagulans T242 was subjected to purification on DEAE chromatography followed by gel permeation chromatography, then the homogenous Bacillus coagulans T242-lactase was obtained, and its molecular mass was 55.0 kDa as shown in SDS-PAGE. Analysis indicated its optimum condition was 60 C and pH 6.8 and it was stable at 40~60 C and pH6.5~7.8; Mn2+, Mg2+ and Na+ at high concentration all had marked activation on lactase activity. Kinetic constants determination revealed Bacillus coagulans T242-lactase had a strong affinity for lactose.

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