Comparison of dynamic fast atom bombardment/liquid secondary ion mass spectrometry and electrospray mass spectrometry coupled to reversed-phase liquid chromatography for the determination of oligosaccharides in seawater

1997 ◽  
Vol 777 (1) ◽  
pp. 141-153 ◽  
Author(s):  
Gustaf Hulthe ◽  
Gunnar Stenhagen ◽  
Elisabet Fogelqvist
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Fast Atom Bombardment ◽  
Secondary Ion Mass Spectrometry ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Secondary Ion

scholarly journals Determination of Alkylphenol Ethoxylates in Textiles by Normal-Phase Liquid Chromatography and Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry

2007 ◽  
Vol 90 (5) ◽  
pp. 1411-1417 ◽  
Author(s):  
Chao Wang ◽  
Qiang Ma ◽  
Xing Wang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Analytical Methods ◽  
Electrospray Mass Spectrometry ◽  
Reversed Phase ◽  
Normal Phase ◽  
Alkylphenol Ethoxylates ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

Abstract Comprehensive analytical methods based on pressurized liquid extraction followed by normal-phase liquid chromatography (NPLC) with ultraviolet detection and reversed-phase liquid chromatography (RPLC)/electrospray mass spectrometry (MS) have been developed for determination of alkylphenol ethoxylates (APEOs) in textile samples. NPLC with an aminosilica column allowed for the chromatographic separation of APEOs according to the increasing number of ethylene units and revealed the exact distribution of individual oligomers. RPLC coupled with electrospray MS was highly sensitive and enabled the complete qualitative and quantitative determination of individual APEOs in textile samples. The 2 analytical methods based on different chromatographic separation mechanisms, i.e., NPLC and RPLC, may provide complementary information of APEOs in textile materials. The 2 detection methods were successfully applied to the investigation of various textile samples, and the data of our research suggested actual pollution in real textile products.

scholarly journals Development and Single-Laboratory Validation of a Reversed-Phase Liquid Chromatography–Electrospray–Tandem Mass Spectrometry Method for Identification and Determination of Acrylamide in Foods

2004 ◽  
Vol 87 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Francesca Calbiani ◽  
Maria Careri ◽  
Lisa Elviri ◽  
Alessandro Mangia ◽  
Ingrid Zagnon
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Food Products ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Cooked Food

Abstract A rapid and accurate method using reversed-phase liquid chromatography–tandem mass spectrometry interfaced with electrospray was developed for determination of acrylamide in cooked food samples. A simplified sample treatment procedure using an extraction step with acidified water without cleanup was developed. A C18 column with an aqueous formic acid–methanol mixture as the mobile phase was used under isocratic conditions. The method was validated in-house for robustness, limits of detection (LOD) and quantitation (LOQ), linearity, recovery, and accuracy both on standard and baked-product and potato flour matrixes. Good results in the low ppb level were obtained for LOD (<15 μg/kg) and LOQ (<25 μg/kg) of acrylamide in samples. Excellent linearity (r 2 = 0.999–1.000) was established over 2 orders of magnitude by performing statistical tests. The absence of both constant and proportional systematic errors demonstrated good method accuracy. Excellent results were obtained for intraday repeatability (RSD < 1.5%) and between-day precision (RSD < 5%). Extraction recoveries from food products were calculated in the 97 ± 3–99 ± 2% (n = 6) range with a labeled internal standard (13C3-acrylamide). The applicability of the method to determination of acrylamide in cooked food products was demonstrated.

scholarly journals Determination of Histamine in Tomatoes by Liquid Chromatography/Mass Spectrometry

2000 ◽  
Vol 83 (3) ◽  
pp. 543-548 ◽  
Author(s):  
Elek Bolygo ◽  
Paul A Cooper ◽  
K Michael Jessop ◽  
Frank Moffatt
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Reversed Phase ◽  
Liquid Chromatography Mass Spectrometry ◽  
Reversed Phase Liquid Chromatography ◽  
Absorbance Detection ◽  
Endogenous Histamine ◽  
Order Of Magnitude ◽  
Phase Liquid

Abstract The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.

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